AKT1 (phospho-Thr450) Antibody
- Known as:
- AKT1 (phosphorilated-Thr450) Antibody
- Catalog number:
- abx000094
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Abbexa
- Gene target:
- AKT1 (phospho-Thr450) Antibody
Related genes to: AKT1 (phospho-Thr450) Antibody
- Gene:
- AKT1 NIH gene
- Name:
- AKT serine/threonine kinase 1
- Previous symbol:
- -
- Synonyms:
- RAC, PKB, PRKBA, AKT
- Chromosome:
- 14q32.33
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: AKT1 (phospho-Thr450) Antibody
Related articles to: AKT1 (phospho-Thr450) Antibody
- Guava ( L.) leaves are valuable functional ingredients; however, evidence linking environmental origin to metabolite-resolved bioactivity remains limited. This study investigated leaves from five Bolivian sites across two ecological regions. While the total phenolic content remained stable, the total flavonoid content and -glucosidase inhibition varied significantly across origins. Widely targeted LC-MS/MS annotated 1230 phenolic features and 753 differential metabolites, revealing an environmental-driven metabolic trade-off: high-irradiance lowlands accumulate lipophilic aglycones, whereas rainforests sequester hydrophilic flavonoid glycosides. Chemometrics revealed origin-dependent chemotypes: Amazonian extracts are glycoside-rich with strong enzymatic inhibition, whereas Lowland extracts are aglycone-enriched for radical scavenging. Network pharmacology and molecular docking prioritized norwogonin, predicting high TNF affinity (-10.1 kcal/mol) and AGE-RAGE axis targeting (e.g., AGER, AKT1, MAPK14). These predictions require experimental validation. Resolving the chemical heterogeneity of Bolivian guava, this work proposes a dual-chemotype classification, providing a robust framework for the strategic sourcing and standardization of functional food resources. - Source: PubMed
Publication date: 2026/08/03
Tao Xin-YuWu Zhou-WeiLiu Xiao-CuiQuan Chen-XiCabero Marco AntonioQiu Ming-Hua - Triple-negative breast cancer (TNBC) is an aggressive subtype that lacks ER, PR, and HER2, which results in limited treatment options and poor outcomes. Lichens are symbiotic organisms known for producing unique secondary metabolites and have a history of use in folk medicine. This study investigated the effects of lichen-derived compounds on MDA-MB-231 cells using integrated in vitro and in silico approaches to explore their anticancer potential and potential molecular targets. Six lichen compounds were isolated, characterized, and tested for antioxidant properties using DPPH and ABTS assays. In vitro cytotoxicity was evaluated on MDA-MB-231 using the MTT and FACS assays. ADMET was evaluated using SwissADME and ADMETLab3; GeneCards was used to retrieve the TNBC-associated genes. The STRING database is used to construct the protein-protein interaction network. Molecular docking analysis was done using CB Dock 2. Structural fluctuation was evaluated in CABS-flex 3.0 web server, and an open-source web-based platform, ShinyGO, was used for functional enrichment analysis. Out of six studied lichens, the extract of Everniastrum cirrhatum showed comparatively stronger antioxidant activity in terms of DPPH and ABTS radical scavenging potential. A decrease of up to 80% in cell viability at 320 µg/mL was observed when treated with Everniastrum cirrhatum, Heterodermia boryi, and Usnea longissima extracts. Six lichen compounds were isolated and characterized. ADMET analysis of isolated compounds suggests that they have acceptable drug-like characteristics with minimal major organ toxicity. The analysis of cytotoxicity using MTT and flow cytometry demonstrated a clear dose-dependent reduction in cell viability by atranorin (ATR), barbatic acid (BAR), and usnic acid (USN). Top 14 TNBC-associated hub genes were identified, and molecular docking analysis suggested that ATR and BAR have favorable binding affinities toward AKT1 and mTOR. RMSF analysis predicted reduced structural fluctuations in selected regions of AKT1 and mTOR following ligand binding, suggesting potential stabilization while preserving overall protein dynamics. KEGG pathway enrichment indicated that these hub genes are enriched in cancer-related pathways, including endometrial cancer and central carbon metabolism in cancer, and are associated with the PI3K/AKT/mTOR and RAS/MAPK signaling pathways. Lichen-derived compounds atranorin, barbatic acid, and usnic acid demonstrated anticancer activity in vitro, while integrated computational analyses suggested potential multitarget interactions that warrant further experimental validation. - Source: PubMed
Publication date: 2026/08/15
Mapari Sachin VGaikwad Subhash BSutar Ruchira RKhare RoshniPatil Ravindra MBehera Bhaskar C - Jianzhong Peiyuan Decoction, based on Shenling Baizhu Powder, is commonly used for stable COPD in Guizhou. However, its chemical composition and pharmacodynamics are not well understood. This study aims to explore its pharmacodynamic basis and multi-target pathways in COPD treatment and to confirm the intervention effect of its key active ingredient, luteolin, through in vitro cell experiments. - Source: PubMed
Publication date: 2026/08/10
Tang AmeiLu KeLiu YangTang GuoxiangCao FengZhou Sufang - Chronic Kidney Disease (CKD) lacks effective disease-modifying therapies. Retinoic acid (RA) exhibits immunomodulatory and antifibrotic properties; however, its molecular targets and structural mechanisms in CKD remain incompletely understood. We applied an integrative strategy combining network pharmacology, structure-based modeling, and in vivo validation. CKD-associated genes were collected from CTD, GeneCards, MalaCards, and TTD, while RA targets were predicted or curated from PharmMapper, SEA, CTD, and STITCH, followed by UniProt harmonization. Kidney-specific expression was ensured using Human Protein Atlas data (nTPM > 1), and disease-relevant targets were prioritized based on upregulation in GSE142025. Functional enrichment analyses were conducted using GO, KEGG, Reactome, and MSigDB Hallmark datasets (FDR q < 0.05). Protein-protein interaction networks were constructed via STRING and analyzed with CytoHubba to identify hub genes. Molecular docking, 100-ns GROMACS molecular dynamics simulations, and MM-GBSA binding free-energy analysis assessed the predicted stability of the RA-target complexes. Experimental validation was performed in a doxorubicin-induced CKD rat model using protein-level analysis. A total of 2,005 CKD genes and 507 RA targets were identified, with 252 overlapping genes, including 216 kidney-expressed targets. Transcriptomic prioritization yielded 86 upregulated targets enriched in immune-inflammatory signaling, apoptosis, and extracellular matrix remodeling. Network analysis identified five hub genes (AKT1, TP53, TNF, FN1, MMP9). Docking predicted moderate-to-strong binding affinities, particularly for MMP9 (- 9.8 kcal/mol). Molecular dynamics simulations indicated stable complexes, and MM-GBSA binding free energies ranked the hubs MMP9 > TNF > p53 > FN1 > AKT1 (- 26.4 to - 12.3 kcal/mol). In vivo, RA significantly modulated renal expression of all hub proteins. These findings identify RA as a context-dependent regulator of immune-fibrotic networks in CKD, with AKT1, TP53, TNF, FN1, and MMP9 as promising therapeutic targets. - Source: PubMed
Publication date: 2026/08/15
Abdelaziz Ayman EShokeir Ahmed AAwad Basem IEl-Khawaga Omali Y - L., known in Arab countries as molokhia, is a leafy plant rich in many carotenoids, minerals, and vitamins; its seeds are also reported to offer cardioprotective effects. Although the cardioprotective effects of molokhia are ascribed to the presence of cardiac glycoside compounds, the exact action mechanisms remain uninvestigated. - Source: PubMed
Publication date: 2026/07/30
Abd El-Mordy Fatma MohamedMaher Sherif ATammam Omar YElrehany Mahmoud AHisham MohamedRofaeil Remon RRateb Mostafa EOraby Hesham FIbrahim Mahmoud A AShawky Ahmed MBringmann GerhardAbdelmohsen Usama RamadanShady Nourhan Hisham