AKT1 (phospho-Thr450) Antibody
- Known as:
- AKT1 (phosphorilated-Thr450) Antibody
- Catalog number:
- abx000094
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Abbexa
- Gene target:
- AKT1 (phospho-Thr450) Antibody
Related genes to: AKT1 (phospho-Thr450) Antibody
- Gene:
- AKT1 NIH gene
- Name:
- AKT serine/threonine kinase 1
- Previous symbol:
- -
- Synonyms:
- RAC, PKB, PRKBA, AKT
- Chromosome:
- 14q32.33
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: AKT1 (phospho-Thr450) Antibody
Related articles to: AKT1 (phospho-Thr450) Antibody
- Keloids are fibroproliferative disorders with excessive extracellular matrix (ECM) deposition, fibroblast hyperproliferation, oxidative stress, and a lack of effective therapies. Shikonin, a natural naphthoquinone, exhibits anti-tumor and antioxidant activities, yet its role in keloid fibroblasts (KFs) remains unclear. In this study, putative Shikonin targets were predicted from public databases and intersected with keloid-associated genes, followed by PPI network construction and GO enrichment analysis. Primary KFs and normal skin fibroblasts (NFs) were treated with Shikonin and assessed for proliferation by CCK-8 assay, apoptosis by flow cytometry, ECM-related protein and HIF-1α expression by Western blotting/immunofluorescence, and intracellular ROS by DCFH-DA staining. Target engagement and functional dependence were validated through molecular docking, molecular dynamics simulations, MM-PBSA analysis, biotin pull-down, CETSA, proteasome inhibition, and EGFR overexpression rescue experiments. Network analysis identified AKT1, EGFR, CASP3, STAT3, BCL2, PTGS2, and HIF-1α as core targets, with enrichment in oxidative stress and PI3K-centered pathways. Shikonin inhibited KF proliferation, induced apoptosis, downregulated Bcl-2 while upregulating Cleaved caspase-3, and suppressed ECM proteins (α-SMA, Fibronectin, Collagen I) and HIF-1α expression. Mechanistically, Shikonin directly bound to EGFR and promoted its proteasomal degradation, consequently suppressing p-AKT, p-STAT3, and PTGS2 expression. EGFR overexpression significantly reversed Shikonin's inhibitory effects on proliferation, ECM production, and oxidative stress, as well as its pro-apoptotic activity. To conclude, Shikonin exerts anti-KF effects by directly targeting EGFR, inducing its proteasomal degradation, and thereby inhibiting downstream AKT/STAT3 signaling and PTGS2 expression. - Source: PubMed
Publication date: 2026/09/28
Yang ZeleiWang MingxiangLi ChengshengChen Huaxia - Xiaoyaosan (XYS) has significant anti-inflammatory effects and is widely used for treating depression. However, the multitarget mechanism of XYS in the treatment of bipolar disorder (BD) remains unexplored. In this study, network pharmacology, molecular docking, and experimental validation were integrated to elucidate the mechanisms of XYS and its therapeutic efficacy in BD. - Source: PubMed
Chen ManDing NanaQiu XingminLi LanGuo YaliWang RongyanqiMo XiaoweiZou TanYu ShangzhenLi XiaojuanChen Jiaxu - Ferroptosis is essential for the occurrence and development of myocardial infarction. The aim of the study is to elucidate the molecular mechanism of Ginkgolide Injection against ischemic cerebral infarction (ICI), focusing on its multi-target regulation of ferroptosis via the AKT1 and NFE2L2. - Source: PubMed
Publication date: 2026/06/11
Lv LuWang JunWang HaigeLin JianruiRuan JiaqiChen Qianchuan - Traditional Chinese medicines (TCMs) are rich sources of structurally diverse bioactive compounds, yet rapid identification of protease inhibitors from complex botanical matrices remains challenging. Here, a magnetic bead-based affinity-fishing platform coupled with UPLC-Q-Exactive-Orbitrap-MS/MS was applied to identify SARS-CoV-2 main protease (M) inhibitors from Reynoutria japonica. The crude ethanolic extract inhibited M with an IC of 44.92 ± 2.31 µg/mL. M-functionalized magnetic beads enriched eight constituents, which were identified by high-resolution mass spectrometry and evaluated using a FRET-based inhibition assay. Six compounds showed concentration-dependent activity, with resveratrol being the most potent (IC = 34.01 ± 1.67 µmol/L), followed by glycitein, genistein, emodin, apigenin-7-O-glucoside, and biochanin A. HPLC-DAD further quantified the principal active constituents. Molecular docking suggested interactions with residues in the Mpro substrate-binding pocket, while network pharmacology predicted associations with host-related targets involving IL6, TNF, and AKT1. Overall, this workflow enables efficient enrichment, structural identification, and functional prioritization of M-inhibitory constituents from complex botanical extracts, providing a practical strategy for target-directed natural product discovery. - Source: PubMed
Huang YujingTian ChenjingGu ZhipengLiang JinlianZu DuntaoYang JianniZhang WanrongRen NinaZheng Junxia - Crinum asiaticum L. has been traditionally used for a long time to treat pain, inflammation, rheumatoid arthritis and other inflammatory disorders. However, the anti-arthritic activity and molecular mechanisms of these effects are not well characterised despite its extensive ethnomedicinal use. The present study investigated the anti-arthritic potential of the methanol extract of C. asiaticum (MECA) using an integrated in-silico, in-vitro and in-vivo approach. The phytochemical profile of MECA was characterized by HRLC-QToF-MS analysis, which tentatively identified 23 phytoconstituents. Network pharmacology identified 69 overlapping targets between MECA associated proteins and rheumatoid arthritis-related genes, while GO and KEGG enrichment analyses highlighted several inflammation related pathways among which the TNF signalling pathway emerged as a prominent enriched pathway associated with the anti-arthritic effects of MECA. Molecular docking supported interactions between selected phytoconstituents and inflammatory targets, while molecular dynamics simulation further supported the stability of the Narciclasine-COX-2 complex. In IL-1β-stimulated SW982 human synovial cells, MECA significantly reduced the production of TNF-α, IL-1β, IL-6, PGE₂, nitric oxide and downregulated the expression of AKT1, COX-2 and IL-6. In the Complete Freund's Adjuvant induced arthritis rat model MECA attenuated paw inflammation and arthritic severity, improved biochemical and inflammatory parameters and protected joint architecture, as confirmed by radiographic and histopathological analyses. Collectively, these findings support the anti-arthritic activity of MECA and indicate that TNF-associated signalling may contribute to its observed anti-inflammatory effects. This study provides scientific evidence supporting the traditional use of C. asiaticum and highlights its potential as a complementary therapeutic candidate for the management of rheumatoid arthritis. - Source: PubMed
Publication date: 2026/09/27
Rath DiptiraniSahoo SubhasishPanda Om ArchitaPattanaik Sandesh KumarLenka Swarna Prava