NTF3 Antibody
- Known as:
- NTF3 Antibody
- Catalog number:
- abx000711
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Abbexa
- Gene target:
- NTF3 Antibody
Related genes to: NTF3 Antibody
- Gene:
- NTF3 NIH gene
- Name:
- neurotrophin 3
- Previous symbol:
- -
- Synonyms:
- NGF2
- Chromosome:
- 12p13.31
- Locus Type:
- gene with protein product
- Date approved:
- 1991-01-15
- Date modifiied:
- 2016-10-05
Related products to: NTF3 Antibody
Related articles to: NTF3 Antibody
- is a renowned flowering shrub, yet the functional studies on the regulatory factors governing its flower color formation remain limited. In this study, a new MYB transcription factor, named , was isolated from the petals of by the homologous cloning technique. The open reading frames of was 705 bp in length. Amino acid sequence analysis showed that contained the highly conserved R2 and R3 MYB domains. A quantitative real-time PCR analysis revealed that was expressed tissue specifically in flowers. Ectopic expression of induced anthocyanins accumulation in both vegetative and reproductive tissues of transgenic tobacco lines. Overexpression of in tobacco enhanced the expression of (MYB) and (bHLH), and the expression of structural genes , , , , , , , and increased remarkably. The UPLC-MS/MS analysis of transgenic tobacco leaves showed that the heterologous expression of significantly promoted the accumulation of anthocyanin metabolites, especially cyanidin, which accounted for more than 50% of the total anthocyanins in the transgenic tobacco lines. This study elucidates the molecular mechanism by which positively regulates anthocyanin biosynthesis in , providing theoretical and technical support for developing plant resources rich in cyanidin. - Source: PubMed
Publication date: 2026/08/17
Yang YinglongDu XinyiYang PanWang BinYe GuangjiLi HuijunZheng Zhenzhen - This study aimed to identify and validate a robust, generalizable panel of plasma protein biomarkers to improve diagnostic precision in Parkinson's disease. We analysed plasma samples from 12 patients with [F]-FP-CIT PET-confirmed Parkinson's disease and 15 healthy controls using the Olink Target 96 Inflammation Panel to identify differentially expressed proteins. Candidate biomarkers were subsequently validated through absolute quantification using Luminex and Olink Flex platforms in an independent cohort of 46 patients with Parkinson's disease and 33 amyloid-negative and cognitively normal control participants. To assess generalizability, the findings were replicated across multiple heterogeneous populations using large-scale datasets from the UK Biobank and Global Neurodegeneration Proteomics Consortium (GNPC) cohorts. Markers of neurodegeneration [neurofilament light chain (NfL)] and Alzheimer's disease [phosphorylated tau (pTau181), amyloid β [Aβ]42, Aβ40] co-pathology were measured using the single molecule array platform. Our analyses revealed elevated levels of interleukin (IL)-10 and IL-17C and reduced levels of urokinase plasminogen activator (uPA) and neurotrophin-3 (NTF3) in patients with Parkinson's disease. These findings were confirmed in the validation cohort. Multitarget models demonstrated superior diagnostic performance over individual markers, with the combination of IL-17C and uPA achieving the highest discrimination (area under the curve = 0.780). External validation in the UK Biobank and GNPC datasets confirmed consistent directional changes of three candidates (IL-17C, NTF3 and uPA), reinforcing the biological relevance of these markers. Notably, while NfL levels were significantly elevated in Parkinson's disease, no significant differences were observed for pTau181 levels or Aβ42/Aβ40 ratios. These findings identify a specific plasma protein panel, particularly the combination of IL-17C and uPA, as a robust and generalizable diagnostic signature that captures fundamental pathophysiological aspects of Parkinson's disease and enhances diagnostic precision alongside established biomarkers. - Source: PubMed
Publication date: 2026/07/16
Lee Ho-WonChoi YoungtaeLee ShinryeKim Jung-EunJeon Min-TaeJo MyungjinPark GyuriPark Jin-SungKim Do-GeunCheon MookyungKim Hyung-Jun - Flavonoid 3',5'-hydroxylase (F3'5'H) is the key enzyme directing metabolic flux toward delphinidin biosynthesis, which is essential for blue flower coloration. However, functional validation of F3'5'H in Clematis-a genus renowned as the "Queen of Climbers"-has not been reported. Here, an interspecific cross between white-flowered Clematis tiantaiensis and blue-purple-flowered C. lanuginosa produced F1 progeny exhibiting color segregation. Two representative offspring-blue-flowered F1-B and blue-purple-flowered F1-PB-were selected for integrated metabolomic and transcriptomic analyses. UPLC-QTOF-MS identified 13 anthocyanins comprising eight delphinidin, four cyanidin, and one petunidin derivatives. At the initial flowering stage, delphinidin derivatives accounted for 71.33% and 54.06% of total anthocyanins as relative percentages of peak area in F1-B and F1-PB, respectively (P < 0.05), whereas cyanidin derivatives showed the inverse pattern. qRT-PCR analysis revealed that ClF3'5'H expression in F1-B was significantly higher than in F1-PB during the critical pigmentation stages (P < 0.01), while ClF3'H showed the opposite trend. Spearman correlation analysis indicated that ClF3'5'H showed the strongest positive association with total anthocyanin content and all delphinidin derivatives (P < 0.0001), consistent with a key role in directing metabolic flux toward delphinidin biosynthesis; however, correlation alone does not imply causation, and functional evidence is provided by the transgenic experiments below. The full-length ClF3'5'H open reading frame (1533 bp, encoding 510 amino acids, CYP75A subfamily) was cloned. Heterologous overexpression in tobacco (Nicotiana tabacum 'NC89') enabled de novo accumulation of delphinidin-3-O-rutinoside, with delphinidin derivatives reaching 17.29% of total anthocyanins. Transgenic tobacco also showed significant upregulation of NtDFR and NtF3'H and downregulation of NtCHS, NtCHI, NtF3H, and NtANS, indicating systemic remodeling of the anthocyanin pathway. These results establish the ClF3'5'H/ClF3'H expression ratio as a molecular determinant of blue-versus-purple divergence in Clematis hybrids and provide key genetic resources for blue flower breeding. - Source: PubMed
Publication date: 2026/07/03
Liu ZhigaoZeng XinliYang HanxiWei DuojieShao Weili - Interstitial cystitis/bladder pain syndrome (IC/BPS) is characterized by complex cellular heterogeneity and inflammatory dysfunction in the bladder. However, the molecular pathogenesis of IC/BPS remains poorly understood, and few studies have provided comprehensive analyses. To elucidate the mechanisms underlying disease pathology, we performed integrated single-cell and spatial transcriptomic analyses of human bladder tissue. Comprehensive single-cell multiomics analyses have identified disease-specific alterations across multiple cell types. T helper 17 cells were significantly expanded in Hunner lesions and activated autoimmune and pro-inflammatory signaling pathways, suggesting a critical role in disease progression. Endothelial cells (ECs) shift from homeostatic (EC2) to pro-angiogenic inflammatory phenotypes (EC3), contributing to vascular remodeling. In the monocyte-macrophage lineage, we identified macrophage 2 cells with pro-angiogenic features that engaged in vascular endothelial growth factor-mediated and tumor necrosis factor-mediated interactions that promoted pathological angiogenesis. Smooth muscle cells (SMCs) display notable phenotypic plasticity, transitioning from a contractile to a synthetic state in IC/BPS tissues. Additionally, spatial transcriptomic analysis revealed upregulation of the EDN1-EDNRA/EDNRB signaling axis, suggesting a role for SMCs in bladder hypercontractility. Fibroblasts have emerged as key mediators of nociceptive signaling, with specific subpopulations enriched in neurotrophin-related pathways (for example, NTF3-NTRK2/NTRK3 and NGF-SORT1) and showing active crosstalk with SMCs, potentially contributing to the development of chronic pain. Collectively, these findings delineate a complex stromal-immune-vascular network underlying the pathological remodeling and pain characteristics of IC/BPS. Our study identified novel cellular players and intercellular signaling pathways that may be promising targets for therapeutic interventions. - Source: PubMed
Publication date: 2026/07/03
Jee ByulAKim IJunLee CheolKim Yeon JeongPark KyungheeSung Ji-HeeHwang InwooKim Jung-SunLee Kyu-SungKo Kwang JinKang Minyong - Since the discovery of nestin-expressing hair follicle-derived cells (NHFCs), they have attracted significant attention for regenerative medicine including neural-tissue engineering. The present study examines the gene expression profiles of NHFCs, focusing on neurotrophic factors and neural differentiation markers, and compares them to those of other well-characterized adult composite stem/progenitor cell systems, including bone marrow-derived mesenchymal cells, dental pulp-derived cells, as well as cortical astrocytes and hypothalamic neurons. The expression of key neurotrophic factors such as Bdnf, Ngf, Vegf, Ntf3, and Gdnf as well as neural-differentiation markers such as Nes, Sox10, Dcx, Tubb3, and Gfap are compared. The present study shows that NHFCs express significantly higher levels of Bdnf, Ngf, and Vegf compared to most of other evaluated cell types, indicating their potential neuroprotective properties. Additionally, NHFCs at passage 4 exhibit lower expression of Nes than the other cell types, suggesting a predisposition to differentiate into mature neuronal lineages. Elevated expression of Tubb3 in NHFCs further indicates the potential for neuronal maturation. The simultaneous expression of both neuronal and glial markers in NHFCs indicates their unique differentiation potential. The present study highlights the distinct molecular signature of NHFCs, suggesting their potential as a valuable resource for neuroregenerative therapies and other regenerative medicine applications. - Source: PubMed
Publication date: 2026/06/04
Salehi Mohammad SaiedAshjazadeh NahidMohammadi YasamanGhorbani NasrinAshayerizadeh AminJafarzadeh Shirazi Mohammad RezaHoffman Robert MPandamooz Sareh