NTF3 Antibody
- Known as:
- NTF3 Antibody
- Catalog number:
- abx000711
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Abbexa
- Gene target:
- NTF3 Antibody
Related genes to: NTF3 Antibody
- Gene:
- NTF3 NIH gene
- Name:
- neurotrophin 3
- Previous symbol:
- -
- Synonyms:
- NGF2
- Chromosome:
- 12p13.31
- Locus Type:
- gene with protein product
- Date approved:
- 1991-01-15
- Date modifiied:
- 2016-10-05
Related products to: NTF3 Antibody
Related articles to: NTF3 Antibody
- This study reports, for the first time, the establishment of endometrial organoids (EOs) from the Arabian camel (Camelus dromedarius) and evaluates their suitability as an in vitro model for embryo-maternal interactions during implantation. Endometrial tissues were collected from non-pregnant she-camels and cultured in Matrigel with a defined growth medium. By Day 7, organoids displayed a spherical morphology (200-250 µm), remained viable for up to 20 days, and expanded to approximately 1 mm. They exhibited epithelial characteristics and high proliferative activity, confirmed by expression of mucin-1, pan-cytokeratin, vimentin, and Ki67. Day 7 in vitro-produced embryos co-cultured with EOs showed significant improvements in development and trophoblast outgrowth. This was accompanied by upregulation of key developmental genes (OCT4, c-MYC, KLF4, CDX2). Cytokine profiling revealed enhanced bidirectional signaling: embryos increased secretion of CCL2, CCL4, IGF-1, IFNG, IL1α, IL12b, IL-8, LIF, IL-10, and NTF3, while EOs upregulated VEGFA, IL-8, CCL2, and TIMP1. Co-culture uniquely induced additional cytokines and amplified signaling intensity. Metabolomic analysis of embryo-conditioned medium identified 108 metabolites, including steroids associated with immunomodulation. Notably, embryos cultured in EO-conditioned medium developed up to Day 21 post-cleavage, reaching a mean diameter of 2.4 mm. Overall, camel EOs provide a physiologically relevant platform that supports embryo development and enables detailed investigation of cytokine-mediated embryo-maternal communication and implantation processes in the dromedary camel. - Source: PubMed
Publication date: 2026/09/02
Saadeldin Islam MAlqassem AlanoudAlomzyan SheemaAlmalki Reem HShinwari ZakiaHama AdelInal SahikaAbdelazim Aaser MAlaiya AyodeleRahman Anas M AbdelAwartani Khalid - Major depressive disorder (MDD) is associated with multiple pathophysiological mechanisms, including dysregulation of monoaminergic systems, excessive glutamatergic activity, reduced neurotrophic support, inflammation, and oxidative stress. The combination of dextromethorphan, an N-methyl-D-aspartate (NMDA) receptor antagonist and sigma-1 receptor agonist, with bupropion, a dopamine/norepinephrine reuptake inhibitor, has been reported to exert rapid antidepressant effects. In the present study, we evaluated the therapeutic effects of the dextromethorphan-bupropion combination on behavioral outcomes and neurotransmitter, inflammatory, and apoptotic pathways in a mouse model of MDD. - Source: PubMed
Publication date: 2026/08/19
Buran Kavuran İlayÖnalan EbruTürk Zeliha İremEröksüz Hatice - is a renowned flowering shrub, yet the functional studies on the regulatory factors governing its flower color formation remain limited. In this study, a new MYB transcription factor, named , was isolated from the petals of by the homologous cloning technique. The open reading frames of was 705 bp in length. Amino acid sequence analysis showed that contained the highly conserved R2 and R3 MYB domains. A quantitative real-time PCR analysis revealed that was expressed tissue specifically in flowers. Ectopic expression of induced anthocyanins accumulation in both vegetative and reproductive tissues of transgenic tobacco lines. Overexpression of in tobacco enhanced the expression of (MYB) and (bHLH), and the expression of structural genes , , , , , , , and increased remarkably. The UPLC-MS/MS analysis of transgenic tobacco leaves showed that the heterologous expression of significantly promoted the accumulation of anthocyanin metabolites, especially cyanidin, which accounted for more than 50% of the total anthocyanins in the transgenic tobacco lines. This study elucidates the molecular mechanism by which positively regulates anthocyanin biosynthesis in , providing theoretical and technical support for developing plant resources rich in cyanidin. - Source: PubMed
Publication date: 2026/08/17
Yang YinglongDu XinyiYang PanWang BinYe GuangjiLi HuijunZheng Zhenzhen - This study aimed to identify and validate a robust, generalizable panel of plasma protein biomarkers to improve diagnostic precision in Parkinson's disease. We analysed plasma samples from 12 patients with [F]-FP-CIT PET-confirmed Parkinson's disease and 15 healthy controls using the Olink Target 96 Inflammation Panel to identify differentially expressed proteins. Candidate biomarkers were subsequently validated through absolute quantification using Luminex and Olink Flex platforms in an independent cohort of 46 patients with Parkinson's disease and 33 amyloid-negative and cognitively normal control participants. To assess generalizability, the findings were replicated across multiple heterogeneous populations using large-scale datasets from the UK Biobank and Global Neurodegeneration Proteomics Consortium (GNPC) cohorts. Markers of neurodegeneration [neurofilament light chain (NfL)] and Alzheimer's disease [phosphorylated tau (pTau181), amyloid β [Aβ]42, Aβ40] co-pathology were measured using the single molecule array platform. Our analyses revealed elevated levels of interleukin (IL)-10 and IL-17C and reduced levels of urokinase plasminogen activator (uPA) and neurotrophin-3 (NTF3) in patients with Parkinson's disease. These findings were confirmed in the validation cohort. Multitarget models demonstrated superior diagnostic performance over individual markers, with the combination of IL-17C and uPA achieving the highest discrimination (area under the curve = 0.780). External validation in the UK Biobank and GNPC datasets confirmed consistent directional changes of three candidates (IL-17C, NTF3 and uPA), reinforcing the biological relevance of these markers. Notably, while NfL levels were significantly elevated in Parkinson's disease, no significant differences were observed for pTau181 levels or Aβ42/Aβ40 ratios. These findings identify a specific plasma protein panel, particularly the combination of IL-17C and uPA, as a robust and generalizable diagnostic signature that captures fundamental pathophysiological aspects of Parkinson's disease and enhances diagnostic precision alongside established biomarkers. - Source: PubMed
Publication date: 2026/07/16
Lee Ho-WonChoi YoungtaeLee ShinryeKim Jung-EunJeon Min-TaeJo MyungjinPark GyuriPark Jin-SungKim Do-GeunCheon MookyungKim Hyung-Jun - Flavonoid 3',5'-hydroxylase (F3'5'H) is the key enzyme directing metabolic flux toward delphinidin biosynthesis, which is essential for blue flower coloration. However, functional validation of F3'5'H in Clematis-a genus renowned as the "Queen of Climbers"-has not been reported. Here, an interspecific cross between white-flowered Clematis tiantaiensis and blue-purple-flowered C. lanuginosa produced F1 progeny exhibiting color segregation. Two representative offspring-blue-flowered F1-B and blue-purple-flowered F1-PB-were selected for integrated metabolomic and transcriptomic analyses. UPLC-QTOF-MS identified 13 anthocyanins comprising eight delphinidin, four cyanidin, and one petunidin derivatives. At the initial flowering stage, delphinidin derivatives accounted for 71.33% and 54.06% of total anthocyanins as relative percentages of peak area in F1-B and F1-PB, respectively (P < 0.05), whereas cyanidin derivatives showed the inverse pattern. qRT-PCR analysis revealed that ClF3'5'H expression in F1-B was significantly higher than in F1-PB during the critical pigmentation stages (P < 0.01), while ClF3'H showed the opposite trend. Spearman correlation analysis indicated that ClF3'5'H showed the strongest positive association with total anthocyanin content and all delphinidin derivatives (P < 0.0001), consistent with a key role in directing metabolic flux toward delphinidin biosynthesis; however, correlation alone does not imply causation, and functional evidence is provided by the transgenic experiments below. The full-length ClF3'5'H open reading frame (1533 bp, encoding 510 amino acids, CYP75A subfamily) was cloned. Heterologous overexpression in tobacco (Nicotiana tabacum 'NC89') enabled de novo accumulation of delphinidin-3-O-rutinoside, with delphinidin derivatives reaching 17.29% of total anthocyanins. Transgenic tobacco also showed significant upregulation of NtDFR and NtF3'H and downregulation of NtCHS, NtCHI, NtF3H, and NtANS, indicating systemic remodeling of the anthocyanin pathway. These results establish the ClF3'5'H/ClF3'H expression ratio as a molecular determinant of blue-versus-purple divergence in Clematis hybrids and provide key genetic resources for blue flower breeding. - Source: PubMed
Publication date: 2026/07/03
Liu ZhigaoZeng XinliYang HanxiWei DuojieShao Weili