KRT20 Antibody
- Known as:
- KRT20 Antibody
- Catalog number:
- abx000633
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Abbexa
- Gene target:
- KRT20 Antibody
Related genes to: KRT20 Antibody
- Gene:
- KRT20 NIH gene
- Name:
- keratin 20
- Previous symbol:
- -
- Synonyms:
- CK20, K20, MGC35423
- Chromosome:
- 17q21.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-02-04
- Date modifiied:
- 2016-03-09
Related products to: KRT20 Antibody
Related articles to: KRT20 Antibody
- Colorectal cancer (CRC) with peritoneal dissemination remains a major therapeutic challenge because of poor prognosis and limited treatment options. Experimental models that accurately recapitulate tumor-mesothelial interactions are scarce. Here, we report the establishment of a novel autologous paired model comprising a CRC cell line (OMUCR-1) and matched cancer-associated mesothelial cells (CAmeso), both simultaneously derived from the malignant ascites of the same patient. Lineage marker analysis using qPCR demonstrated that OMUCR-1 selectively expressed epithelial markers (EPCAM, KRT20), whereas CAmeso strongly expressed mesothelial-mesenchymal markers (ACTA2, MSLN) and lacked epithelial marker expression. These mutually exclusive expression patterns confirm that the two cell populations are phenotypically distinct and rule out cross-contamination. OMUCR-1 displayed strong tumorigenic capacity across multiple transplantation models. CAmeso enhanced CRC cell migration and invasion in vitro, and co-transplantation with OMUCR-1 resulted in larger tumors enriched with αSMA-positive stromal components. RNA sequencing of co-injected xenografts revealed increased expression of murine stromal Fgfr3. Treatment with the FGFR inhibitor BGJ398 reduced tumor growth and decreased stromal FGFR3-positive components, suggesting that stromal FGFR3 may represent a potential microenvironmental vulnerability in CRC with peritoneal dissemination. This autologous CRC-mesothelial system provides a physiologically relevant platform for dissecting tumor-stroma interactions in peritoneal metastasis and may advance stromal-targeted therapeutic strategies. - Source: PubMed
Fukui YasuhiroKasashima HiroakiWang ZizhouOmori IguruKusunoki YukinaEchizen KanaeNonaka YoshikiSeki YukiKuroda KenjiMiki YuichiroYoshii MamiFukuoka TatsunariTamura TatsuroShibutani MasatsuneToyokawa TakahiroMuta YuNakanishi YukiYashiro MasakazuOhtani NaokoMaeda Kiyoshi - BackgroundSignet-ring cell adenocarcinoma of the urinary bladder is a rare and aggressive malignancy. Complete colonic metaplasia of urinary bladder is exceedingly rare, with only 11 patients reported in English literature.PresentationA 56-year-old woman presented with a 6-month history of voiding difficulty and hematuria. She underwent radical cystectomy with hysterectomy, bilateral salpingo-oophorectomy, and pelvic lymph node dissection, and was ultimately diagnosed as primary signet-ring cell adenocarcinoma of the urinary bladder. The entire bladder mucosa, including the ureteric mucosa, showed complete colonic metaplasia. Immunohistochemistry demonstrated a lower gastrointestinal-type immunophenotype, with positivity for KRT20 and CDX2.DiscussionThis report highlights an extremely rare instance of complete colonic metaplasia of the urothelium with subsequent development of signet-ring cell adenocarcinoma. - Source: PubMed
Publication date: 2026/04/10
Mitra SaikatShangloo AksharaBhatkule Milind AAkif Shiraz - To study the clinicopathological features and key differential diagnosis of clear cell adenocarcinoma (CCA) of the urinary tract. A retrospective analysis was performed on the clinicopathological data, immunophenotypes, and molecular test results of patients with pathologically confirmed primary CCA of the urinary tract at Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China from December 2015 to September 2024. This study was supplemented by a review of the relevant literature. Nine patients were included (1 male and 8 females), age 57.0 (44.5, 61.0) years old. The main symptoms were urinary tract irritation and hematuria. Th lesions were in the urethra of 5 patients and in the bladder in 4 patients. Macroscopically, the tumors were cauliflower-like, with edema and firm consistency. Microscopically, they showed mostly papillary/tubular-cystic structures, with solid sheet-like arrangements. The tumor cells showed clear or eosinophilic cytoplasm, moderate to high nuclear grade, prominent nucleoli, and frequent mitotic figures. The tumor cells also exhibited strong expression of PAX8, CK7, and HNF1-β, various positivity of Napsin A and P504s, and partial expression of SOX17, CK20, and GATA3. The Ki-67 index ranged from 30% to 70%, and p53 showed a heterogeneous expression pattern. The cells were negative for p63 and the renal cell carcinoma marker (RCC). Among the 7 cases subject to urine fluorescence in situ hybridization (FISH) testing, 5 cases showed abnormal centromeric signals. One case underwent next-generation sequencing (DNA-seq) and harbored a nonsense ARID1A mutation. Two patients underwent radical surgery, 3 total urethrectomy, and 4 palliative surgery. Four patients received postoperative chemotherapy. The follow-up ranged from 9 to 58 months: 3 patients had tumor recurrence while 2 died. CCA of the urinary tract is a rare and aggressive malignancy. Its histological morphology resembles that of ovarian CCA. The diagnosis should be based on a combination of morphological features and immunophenotype. It is recommended to use PAX8, CK7, HNF1-β, and Napsin A as a core immunohistochemical panel, while testing SOX17 can also help. - Source: PubMed
Zeng W RXu A WCheng Z LYong J JLi Z - Blueberry anthocyanins, a class of flavonoids with antioxidant and anti-inflammatory properties, were investigated for their role in enhancing intestinal barrier, comparing differential effects between immature and mature enterocytes. Using both wild-type (WT) and Toll-like receptor 4 gene knockout (TLR4) mice at pup and adult stages, blueberry anthocyanin extract (BAE) was administered via oral gavage, and an ex vivo inflammatory model was established with interleukin-1beta (IL-1β) stimulation. Analysis of 16S rDNA sequencing revealed that BAE modulated gut microbiota in an age- and TLR4-dependent manner, increasing alpha diversity in WT pups but reducing in adults, enriching beneficial Akkermansia in adults. Additionally, BAE elevated total short-chain fatty acid (SCFA) levels, with higher concentrations in WT mice than in TLR4 mice. The SCFA profile was developmentally regulated, marked by the absence of acetate in pups and a BAE-specific induction of valerate in adults. The improvement of the intestinal barrier by BAE was also evidenced by the upregulation of tight junction (TJ) proteins, as well as the suppression of IL-1β-induced pro-inflammatory cytokines and inflammatory mediators. These effects were abolished in TLR4 mice, and pups showed more pronounced inflammatory sensitivity. Furthermore, comparisons between distinct developmental stages revealed that BAE specifically supports the maturation and homeostasis of the intestinal epithelium. Especially, BAE exhibited a distinct pattern of regulation between embryonic-type (e.g., Krt18 and Hbb-γ) and adult-type genes (e.g., Krt20 and Hbb-b1). These findings indicate TLR4 as a critical target, proposing the application of blueberry anthocyanins as a functional ingredient to prevent intestinal barrier dysfunction across life stages. - Source: PubMed
Publication date: 2026/03/14
Xing YakunZhao XingyuZheng JiaweiLi XinyuHuang Wuyang - Villous adenoma (VA) of the renal pelvis is a rare intestinal epithelial neoplasm. It generally arises in elderly men and is frequently associated with chronic irritation, infection, or long-standing obstruction, most often due to nephrolithiasis or hydronephrosis. We report four previously unpublished patients diagnosed with VA of the renal pelvis. Each patient was evaluated clinicopathologically, radiologically, immunohistochemically to clarify morphologic diversity and biological behavior. We also review previously reported patients in the literature. All four patients were men, aged 45-70 years, each with common history of chronic infection or calculi. Radiological findings revealed advanced hydronephrosis and mucin-filled collecting systems (muconephrosis). Grossly, the lesions were mucinous and cystically dilated. Microscopically, the lesions from Patient 1 and 2 showed classic villiform mucinous epithelium without invasion; the lesion from Patient 3 exhibited serrated adenoma-like architecture with dysplastic nuclei; the lesion from Patient 4 demonstrated low-grade dysplasia with preserved crypt morphology. Immunohistochemically, all lesions expressed intestinal markers including KRT20, CDX2, SATB2, with variable KRT7 expression, wild-type p53 (TP53) expression. None of the lesions showed invasive growth or recurrence during follow-up. VA of the renal pelvis represents an intestinal-type lesion of upper urinary tract that develops in setting of chronic irritation and mucus accumulation and may be associated with intestinal metaplasia. Because its clinical and radiologic features often mimic inflammatory or cystic disease, histopathologic evaluation is essential for accurate diagnosis. Recognition of this rare entity is crucial to differentiate it from mucinous adenocarcinoma, as complete surgical excision is curative and long-term prognosis is excellent. - Source: PubMed
Publication date: 2026/03/23
Yaprak Bayrak BusraOznur MeltemSeday Suheda ZeynepAydın MeryemKiran Merve MeryemAltıntas SuleymanAkcalı NilAkgul Mahmut