DOK4 Antibody
- Known as:
- DOK4 Antibody
- Catalog number:
- abx000611
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Abbexa
- Gene target:
- DOK4 Antibody
Related genes to: DOK4 Antibody
- Gene:
- DOK4 NIH gene
- Name:
- docking protein 4
- Previous symbol:
- -
- Synonyms:
- FLJ10488
- Chromosome:
- 16q21
- Locus Type:
- gene with protein product
- Date approved:
- 2002-11-28
- Date modifiied:
- 2016-10-05
Related products to: DOK4 Antibody
Related articles to: DOK4 Antibody
- Largemouth bass (Micropterus salmoides), an important species in both aquatic ecosystems and aquaculture, is increasingly affected by heat extremes. Heat stress poses significant challenges to fish health, particularly affecting key organs like the gills. This study investigated the effects of heat stress (37 °C) on the gills of juvenile largemouth bass, focusing on histopathology and molecular adaptations, including genome-wide gene expression and DNA methylation. After the heat stress experiment, histological analysis of hematoxylin and eosin-stained gill tissues revealed significant alterations, including increased blood cell density, epithelial cell proliferation, and curling of gill lamellae. Ultrastructural analysis via transmission electron microscopy showed irregular cell shapes of the secondary lamellae. TUNEL staining demonstrated a significant increase in apoptotic cells, from 14.53 % in control fish versus 69.58 % in heat-stressed fish, which correlated with upregulation of apoptosis-related genes, such as caspase3 and p53. Transcriptomic analysis using RNA sequencing identified 608 differentially expressed genes. KEGG enrichment analysis revealed significant enrichment of the "protein processing in endoplasmic reticulum" pathway. Additionally, whole-genome bisulfite sequencing and methylomic analysis identified 12,505 differentially methylated regions. GO enrichment analysis of genes containing promoter DMRs identified 44 significantly enriched terms, including "metabolic process," "organic substance metabolic process," "binding" and "protein interaction." Notably, genes associated with apoptosis, such as capn3a, dok4, rassf7a, and tusc2a, exhibited significant changes in both gene expression and DNA methylation in their promoters. These findings provide insights into the molecular responses of fish gills to heat stress and mechanisms of resilience in cultured fish species under changing environmental conditions. - Source: PubMed
Publication date: 2025/03/17
Liang SichengZhang GenrongZhu HaoWei YekaiSun YiLin ZijieLing Qufei - Tuberculosis (TB) remains a major global health concern, with the transition from latent to active TB still poorly understood. Therefore, enhancing clinical management and prevention strategies for TB is essential. High-throughput sequencing data of genes and miRNAs from individuals at different TB stages were obtained from NCBI. Differential expression analysis was performed using the R package limma, alongside GO and KEGG analyses. The central regulatory network of miRNAs was visualized with Cytoscape, and relevant genes were validated using ROC analysis. The predicted key genes involved in the transition from latent to active TB, including PLEKHG1, CLPB, DOK4, IL1β, and TLR3, are primarily associated with multicellular organism processes, stimulus-response, GPCR ligand binding, and immune functions. Finally, we screened Celastrol and Cefaclor Anhydrous targeting IL1β as potent anti-inflammatory drug to reduce the inflammation due to TB. These findings were further validated with Molecular dynamic simulation MM-GBSA and PCA analysis. Our study advances the understanding of latent tuberculosis and identifies genes and microRNAs as potential biomarkers for diagnosis, monitoring, and treatment, with broader implications for complex disease research. - Source: PubMed
Publication date: 2025/02/16
Dinh PhongSonTran ChauMyThanhDinh ThiPhuongHoaiHa Hai-AnhUtegenova AigulAli AwaisAlamri Abdulaziz - Stroke remains a leading cause of mortality and long-term disability worldwide, underscoring the urgent need to identify novel therapeutic targets to enhance brain circuitry repair and functional recovery. This study explores the concept of longevity assurance genes, which primarily function within genetic pathways responsible for repair and maintenance. These pathways encompass molecular and metabolic processes as well as organ- and system-level functions. To investigate this, we employed comparative transcriptomics to analyze gene expression patterns across three age groups with progressively decreasing brain plasticity: native postnatal day seven brains, and young and old naïve and lesioned rat male brains. Analysis revealed a highly symmetrical distribution of upregulated and downregulated genes in postnatal day 7 brains. In contrast, the gene expression profiles of post-stroke brains exhibited significant asymmetry, with a disproportionate increase in upregulated genes compared to downregulated ones in both young and old post-ischemic brains. Gene variance in juvenile brains predominantly reflected processes associated with brain plasticity (e.g., Dcx, Tubb2b, Dok4, Dpysl5) and cell proliferation (e.g., Bex4). Conversely, gene expression variance in young and aged post-stroke brains was largely linked to inflammatory pathways, driven by cytokine and chemokine signaling. Notably, several genes specifically upregulated in aged brains were identified, including Ehd4, Fut7, Lilrb4, Plek, Slfn13, Slc14a1, and Smpdl3a. Immune genes that facilitate synaptic plasticity during early postnatal brain development-through processes such as pruning and sprouting to establish new connections in response to external stimuli-also contribute to post-stroke damage, confirming the concept of antagonistic pleiotropy. Our results suggest that targeting age-related immune responses could be an effective therapeutic strategy for stroke recovery. - Source: PubMed
Publication date: 2025/02/18
Pirscoveanu Denisa F VOlaru Denissa GretaHermann Dirk MDoeppner Thorsten RGhinea Flavia SemidaPopa-Wagner Aurel - Prevention of human hypertension is an important challenge and has been achieved in experimental models. Brief treatment with renin-angiotensin system (RAS) inhibitors permanently reduces the genetic hypertension of the spontaneously hypertensive rat (SHR). The kidney is involved in this fascinating phenomenon, but relevant changes in gene expression are unknown. - Source: PubMed
Byars Sean GPrestes Priscilla RSuphapimol VarapornTakeuchi FumihikoDe Vries NathanMaier Michelle CMelo MarianaBalding DavidSamani NileshAllen Andrew MKato NorihiroWilkinson-Berka Jennifer LCharchar FadiHarrap Stephen B - Goats contribute significantly to the global food security and industry. They constitute a main supplier of meat and milk for large proportions of people in Egypt and worldwide. Brucellosis is a zoonotic infectious disease that causes a significant economic loss in animal production. A case-control genome-wide association analysis (GWAS) was conducted using the infectious status of the animal as a phenotype. The does that showed abortion during the last third period of pregnancy and which were positive to both rose bengal plate and serum tube agglutination tests, were considered as cases. Otherwise, they were considered as controls. All animals were genotyped using the Illumina 65KSNP BeadChip. Additionally, the diversity and composition of vaginal and fecal microbiota in cases and controls were investigated using PCR-amplicone sequencing of the V4 region of 16S rDNA. After applying quality control criteria, 35,818 markers and 66 does were available for the GWAS test. The GWAS revealed a significantly associated SNP (P = 5.01 × 10) located on Caprine chromosome 15 at 29 megabases. Four other markers surpassed the proposed threshold (P = 2.5 × 10). Additionally, fourteen genomic regions accounted for more than 0.1% of the variance explained by all genome windows. Corresponding markers were located within or in close vicinity to several candidate genes, such as ARRB1, RELT, ATG16L2, IGSF21, UBR4, ULK1, DCN, MAPB1, NAIP, CD26, IFIH1, NDFIP2, DOK4, MAF, IL2RB, USP18, ARID5A, ZAP70, CNTN5, PIK3AP1, DNTT, BLNK, and NHLRC3. These genes play important roles in the regulation of immune responses to the infections through several biological pathways. Similar vaginal bacterial community was observed in both cases and controls while the fecal bacterial composition and diversity differed between the groups (P < 0.05). Faeces from the control does showed a higher relative abundance of the phylum Bacteroidota compared to cases (P < 0.05), while the latter showed more Firmicutes, Spirochaetota, Planctomycetota, and Proteobacteria. On the genus level, the control does exhibited higher abundances of Rikenellaceae RC9 gut group and Christensenellaceae R-7 group (P < 0.05), while the infected does revealed higher Bacteroides, Alistipes, and Prevotellaceae UCG-003 (P < 0.05). This information increases our understanding of the genetics of the susceptibility to Brucella in goats and may be useful in breeding programs and selection schemes that aim at controlling the disease in livestock. - Source: PubMed
Publication date: 2023/11/16
Sallam Ahmed MAbou-Souliman IbrahimReyer HenryWimmers KlausRabee Alaa Emara