Polyclonal Rabbit DGCR8 Antibody
- Known as:
- Polyclonal Rabbit DGCR8 Antibody
- Catalog number:
- abx034165
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Abbexa
- Gene target:
- Polyclonal Rabbit DGCR8 Antibody
Related genes to: Polyclonal Rabbit DGCR8 Antibody
- Gene:
- DGCR8 NIH gene
- Name:
- DGCR8 microprocessor complex subunit
- Previous symbol:
- C22orf12
- Synonyms:
- DGCRK6, Gy1, pasha
- Chromosome:
- 22q11.21
- Locus Type:
- gene with protein product
- Date approved:
- 2000-06-29
- Date modifiied:
- 2019-01-25
Related products to: Polyclonal Rabbit DGCR8 Antibody
Related articles to: Polyclonal Rabbit DGCR8 Antibody
- MicroRNAs are small non-coding RNAs that mediate post-transcriptional silencing of most mammalian genes. They are generated in a multi-step process initiated by the Microprocessor, a protein complex composed of DROSHA and DGCR8. Recent studies have described the phenomenon of "cluster assistance," in which a prototypic primary miRNA hairpin can license the Microprocessor-mediated processing of a clustered suboptimal hairpin in cis. Genetic screening and mechanistic analyses led to the identification of two critical factors for this process, SAFB2 (scaffold attachment factor B2) and ERH (enhancer of rudimentary homolog), which have been shown to associate with the N-termini of DROSHA and DGCR8, respectively, but also form a complex with each other. However, it remains unclear how SAFB2 and ERH can alter the Microprocessor substrate specificity and whether the described protein-protein interactions are required for cluster assistance. In this study, we focused on the role of ERH and showed that its loss largely phenocopies the effect of SAFB1/2 deletion on the miRNA transcriptome, suggesting that both factors are involved in the same processes of primary miRNA biogenesis. In this context, our data demonstrate that both SAFB1/2 and ERH are required for efficient Microprocessor feedback regulation via processing of pri-miR-1306, uncovering a clear physiological function of cluster assistance. Mechanistically, our data show that ERH-mediated cluster assistance depends neither on its direct association with SAFB2 nor on its described interaction with DGCR8. In contrast, disrupting the ERH binding site within DGCR8 drives the processing of a subset of cluster assistance-unrelated pri-miRNAs. Thus, this study reveals dual roles of ERH in primary miRNA biogenesis, a largely suppressive one driven by its direct binding to DGCR8, and the other in cluster assistance that does not require DGCR8 binding. - Source: PubMed
Aschenwald SimonPanda Aswini KWurzer TheresaBaumgärtner SonjaErtl Valerie SHatzer JakobVillunger AndreasFalk SebastianHerzog Sebastian - ADAR1 is overexpressed in hepatocellular carcinoma (HCC) and has been linked to poor prognosis, metastasis, and recurrence; however, its precise functions and underlying mechanisms, particularly in the context of metastasis, remain inadequately elucidated. This study seeks to elucidate the functions and underlying mechanisms of the most abundantly expressed isoform of ADAR1, ADAR1p110, in the setting of HCC metastasis. In this study, hepatocyte-specific ADAR1p110 knock-in mice and engineered HCC cell lines were utilized to investigate the function of ADAR1p110 and . Genome sequencing, transcriptome sequencing, microRNA sequencing, RNA immunoprecipitation qPCR (RIP-qPCR), and RNA pull-down assays were performed to elucidate the mechanism of ADAR1p110 in HCC. We demonstrated that ADAR1p110 overexpression promotes HCC metastasis by improving the motility of HCC cells. Mechanistically, ADAR1p110 overexpression increases TUBA1A expression, which plays a crucial role in regulating HCC cell motility. At the molecular level, ADAR1p110 suppresses miR-451a biogenesis by competitively binding to pri-miR-451a, thereby preventing its cleavage by the Drosha/DGCR8 complex. Furthermore, we confirmed that TUBA1A is a direct downstream target of miR-451a. - Source: PubMed
Publication date: 2025/07/12
Sun LiangzhanYang HuiHu PengchaoZheng JingyiDu YuyangWu ShashaGao HanLuo HaoWang YanchenWang FenfenYan JingsongGuan Xin-YuanLi Yan - Silicosis, one of the most common and severe forms of pneumoconiosis, remains a major occupational health concern worldwide. Given the lack of effective therapies, understanding the underlying molecular mechanisms is urgently needed. Here, we report that ALKB homolog 1 (ALKBH1), an N6-methyladenosine (mA) demethylase, is upregulated in silica-induced pulmonary fibrosis and plays a pro-fibrotic role. The antifibrotic peptide Ac-SDKP inhibited Alkbh1 expression and alleviated pulmonary fibrosis. Mechanistically, ALKBH1 suppressed the biosynthesis of miR-129-5p by removing mA modification from pri-miR-129-5p, thereby reducing DGCR8-mediated processing and leading to decreased mature miR-129-5p levels. Ac-SDKP reversed this process, restoring miR-129-5p expression. Functionally, overexpression of miR-129-5p attenuated silica-induced pulmonary fibrosis by suppressing macrophage activation. Collectively, these findings identify the Ac-SDKP-ALKBH1-miR-129-5p axis as a critical regulatory mechanism, with ALKBH1-mediated mA demethylation of pri-miR-129-5p representing a key node and a promising therapeutic target for silicosis. - Source: PubMed
Li QianDu JiakunYi XueLi ShifengYang YiWang XinyuXu ZelinJin FuyuLi TianLi YaqianXu DingjieWei ZhongqiuCai WenchenMao NaZhang LijuanYu XiaoShi YiweiYang FangXu HongGao Xuemin - Overcoming chemoresistance is a major therapeutic challenge to improve patient's outcome, and lncRNAs are involved in the carcinogenesis and progression of NSCLC. Accordingly, this study was aimed to explore the roles and functions of lncRNA FENDRR in the progression and drug resistance of NSCLC. - Source: PubMed
Publication date: 2026/04/15
Wang Yuan-JinLiu Xiang-MingLi Shu-YuanLv Meng-WeiWang QiaoZhang Hao - Molecular biomarkers are increasingly used for risk stratification, particularly in up-front surgery settings (Children's Oncology Group trials), whereas in preoperative chemotherapy setting, the ongoing International Society of Pediatric Oncology (SIOP)-Renal Tumor Study Group-2016 UMBRELLA study aims to validate selected biomarkers for future risk-adapted treatment strategies. This systematic review summarizes all literature on the prognostic value of these biomarkers. - Source: PubMed
Publication date: 2026/05/18
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