IRF1 293T Cell Transient Overexpression Lysate(Denatured)
- Known as:
- IRF1 293T Cell Transient Overexpression Lysate(Denatured)
- Catalog number:
- H00003659-T01
- Product Quantity:
- 100 uL
- Category:
- -
- Supplier:
- Abno
- Gene target:
- IRF1 293T Cell Transient Overexpression Lysate(Denatured)
Related genes to: IRF1 293T Cell Transient Overexpression Lysate(Denatured)
- Gene:
- IRF1 NIH gene
- Name:
- interferon regulatory factor 1
- Previous symbol:
- -
- Synonyms:
- MAR
- Chromosome:
- 5q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 1991-05-09
- Date modifiied:
- 2016-10-05
Related products to: IRF1 293T Cell Transient Overexpression Lysate(Denatured)
Related articles to: IRF1 293T Cell Transient Overexpression Lysate(Denatured)
- Interferon regulatory factors (IRFs) are DNA-binding transcription factors involved in immune regulation, yet the composition and physiological relevance of IRF repertoires in bivalve molluscs remain incompletely understood. In this study, comparative genomic, phylogenetic, structural and transcriptomic analyses were integrated to examine IRF evolution and immune-associated expression in bivalves. IRF genes were identified from representative bivalve proteomes and analyzed together with IRFs from model animals and previously characterized molluscan sequences. The dataset comprised 29 bivalve species from 12 families. Within this sampled dataset, IRF copy number ranged from two to four per species, with most species retaining three members, indicating a restricted IRF repertoire. Phylogenetic analysis of 113 IRF proteins from 34 species resolved four major subfamilies. Bivalve IRFs were mainly assigned to IRF1/2-like and IRF4/8/9-related lineages, whereas in the present dataset, clear bivalve representatives of vertebrate IRF3/7 and IRF5/6 clades were not detected. Motif and gene-structure analyses indicated conservation of the N-terminal IRF DNA-binding region, while the middle and C-terminal regions were more variable, particularly in IRF4/8-like members. In the noble scallop Mimachlamys nobilis, IRF genes and selected IRF-related genes, including MyD88, TRAF6-like, TBK1/IKKε-like and IKKα/β-like candidates, showed gene-specific expression patterns across tissues, with several genes reaching their highest mean expression in the gill, and showed distinct temporal expression profiles over 72 h of Vibrio parahaemolyticus exposure. Re-analysis of oyster and mussel transcriptomes further showed that IRF genes were expressed together with adaptor and kinase genes under bacterial exposure or in gill-associated immune contexts. These findings suggest that bivalves maintain a restricted but immune-relevant IRF repertoire, and that the potential functional diversification of molluscan IRFs is likely shaped by structural divergence, tissue-biased deployment and differential association with upstream innate immune signaling components rather than by extensive gene-family expansion. - Source: PubMed
Publication date: 2026/08/25
Liao WeiminXie ZekunLiu ShitongSu HailongHu HaixinHe QiqiXu TianyiZhang HongkuanZheng Huaiping - The STING1-IRF1 axis plays a crucial role in sensing innate immunity within the tumor microenvironment. However, the genomic landscape of this axis and its clinical implications in breast cancer still require a deeper understanding, more comprehensive explanation, and greater detail. - Source: PubMed
Publication date: 2026/08/01
Alhamadani IsraAlzeyadi Mohammad - Pancreatic ductal adenocarcinoma (PDAC) exhibits extensive epithelial plasticity, yet epithelial states associated with interferon signaling remain incompletely defined. We analyzed cleaned epithelial cells classified as singlets in two independent PDAC single-cell RNA-sequencing cohorts, GSE155698 and GSE212966, and constructed a composite score combining an interferon-associated module (IFNcore) with an epithelial-secretory module (Secretorycore). VM-high cells consistently co-expressed representative genes from both programs. Within individual PDAC tumors, IFNcore and Secretorycore were positively associated in 14 of 17 GSE155698 samples and 5 of 6 GSE212966 samples. Sample-level effect sizes and leave-one-gene-out analyses further identified IRF1, STAT1, and ELF3 as candidate factors associated with distinct components of the state. The principal findings were preserved after doublet filtering and remained detectable in Harmony- and CopyKAT-based robustness analyses. In the independent GSE62452 cohort, the composite score correlated with a non-overlapping interferon signature among 69 PDAC tumors (Spearman rho = 0.584, P = 1.42 × 10^-7). In TCGA-PAAD, a higher continuous score was associated with poorer overall survival in univariate Cox analysis, although the association was attenuated after adjustment for age, sex, stage, and grade. These findings define a reproducible PDAC epithelial transcriptional state in which interferon-associated activity coexists with secretory epithelial identity and nominate IRF1, STAT1, and ELF3 for subsequent functional investigation. - Source: PubMed
Publication date: 2026/08/12
Mi XingyuWu ShifanXiao Liang - Obesity-associated metabolic disorders are characterized by impaired glucose and lipid metabolism, insulin resistance, and adipose tissue dysfunction. Interferon regulatory factor 1 (IRF1) is a transcription factor primarily involved in immune regulation; however, its role in adipocyte metabolic regulation remains incompletely understood. In this study, adipocyte-specific knockout ( AKO) mice were generated using the Cre/loxP system and subjected to either a regular chow diet or a high-fat diet (HFD). Metabolic phenotyping, insulin signaling analysis, mitochondrial homeostasis-related assessment, and in vitro adipocyte experiments were performed. Adipocyte-specific IRF1 deficiency improved insulin-stimulated AKT phosphorylation in white adipose tissues and enhanced glucose tolerance and insulin sensitivity under HFD conditions. These metabolic improvements were accompanied by reduced oxygen consumption, energy expenditure, heat production, β3-adrenergic-induced lipolytic response, and cold tolerance. At the molecular level, IRF1 deficiency was associated with reduced TOMM20 expression, decreased mtDNA content, downregulation of oxidative phosphorylation-related genes, and reduced ATP levels in adipose tissues, suggesting altered mitochondrial homeostasis. In 3T3-L1 adipocytes, IRF1 knockdown increased insulin-stimulated AKT activation, glucose uptake, and lipid accumulation, whereas IRF1 overexpression showed opposite trends. Collectively, these findings suggest that adipocyte IRF1 is associated with insulin signaling, lipid metabolic remodeling, and mitochondrial homeostasis, and highlight a potential dissociation between improved insulin responsiveness and reduced energy expenditure in diet-induced obesity. - Source: PubMed
Publication date: 2026/08/17
Zhu AiranZhou YingRen KailiSun ChongXiu - Heme oxygenase-1 (HO-1), encoded by , catalyzes the rate-limiting step of heme degradation and generates biliverdin, carbon monoxide, and ferrous iron, thereby linking heme turnover with redox regulation and stress-associated signaling. In birds, biliverdin is retained as a major heme-derived product, but the cellular consequences of perturbation remain insufficiently defined. Here, CRISPR/Cas9-mediated editing was used to generate a heterogeneous -edited population in Chicken hepatocellular carcinoma-derived cells. The selected sgRNA reduced HO-1 protein abundance by approximately 47%, and no detectable cleavage was observed at the seven predicted high-risk off-target loci examined. Compared with vector-control cells, -edited cells exhibited intracellular heme accumulation, reduced biliverdin levels, increased oxidation-sensitive fluorescence, and reduced CCK-8 absorbance values, indicating disruption of heme-biliverdin metabolic and redox homeostasis. RNA sequencing identified 2650 differentially expressed genes, including 951 upregulated and 1699 downregulated genes. Downregulated genes were mainly enriched in immune, cytokine, MAPK/stress, and extracellular signaling-associated pathways, whereas DNA replication and cell-cycle-related genes were increased. Enrichment-term association and STRING functional-association analyses further identified a coordinated module involving , , , , , , , and . Independent RT-qPCR analysis confirmed selected expression trends. These findings show that heterogeneous editing and reduced HO-1 abundance are associated with disruption of the avian heme-biliverdin metabolic axis and coordinated remodeling of basal immune, stress, extracellular signaling, and cell-cycle-associated transcriptional programs in Chicken hepatocellular carcinoma-derived cells. - Source: PubMed
Publication date: 2026/08/08
Tang HaonanLi HuaiyuTai YurongYang XueZhang LetianMa YuhaoCai GanxianZhao HongyangZeng TongAi XiaohuaHe ShuangWang JiankuiGu ZhiliangDeng Xuemei