PEDF, Human Protein
- Known as:
- PEDF, Human Protein
- Catalog number:
- z02722-1
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Genscript
- Gene target:
- PEDF Human Protein
Related genes to: PEDF, Human Protein
- Gene:
- SERPINF1 NIH gene
- Name:
- serpin family F member 1
- Previous symbol:
- PEDF
- Synonyms:
- EPC-1, PIG35
- Chromosome:
- 17p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1993-05-18
- Date modifiied:
- 2018-03-02
Related products to: PEDF, Human Protein
Related articles to: PEDF, Human Protein
- To describe the clinical symptoms, obesity status and body composition distribution in children with osteogenesis imperfecta (OI). This retrospective cohort study included 45 children aged 0-18 years with OI, diagnosed by clinical and genetic testing at Children's Hospital, Zhejiang University School of Medicine, from October 2020 to February 2025. Clinical data were collected on age, sex, symptoms, imaging examinations and genetic testing results. According to body mass index (BMI) criteria or weight-for-length Z-score, the OI patients were divided into non-overweight or obese group and overweight or obese group. Concurrently, 60 children with simple overweight or obesity, matched for sex, age and BMI, were enrolled as a control group. The -test and Mann-Whitney test were used to compare the clinical characteristics between the 2 OI groups, and body composition (lean mass index (LMI)and fat mass index (FMI)) between the overweight or obese OI group and the control group. Fracture frequency distribution between the two OI groups were compared using the ² test. Of the 45 OI children, 26 were male and 19 female, with an average age of (7.2±3.9) years. Genetic variants involved COL1A1 or COL1A2 in 40 cases, IFITM5 in 3, SERPINF1 in 1, and BMP1 in 1. Of the OI patients, 18 (40%) were in the overweight or obesity group and 27 (60%) in the non-overweight or obesity group; 19 (42%) had short stature or growth retardation. Between the 2 OI groups, none of sex, osteocalcin, bone mineral density Z-score, or vitamin D level differed significantly (all >0.05), whereas fracture frequency did differ significantly (<0.05). Twenty seven OI children (13 in the overweight or obesity group and 14 in the non-overweight or obesity group) completed the dual-energy X-ray absorptiometry, and all of them had COL1A1 or COL1A2 genotypes. The FMI level was higher in the overweight or obese group than in the non-overweight or obese group (9.0 (6.1, 9.6) 4.4 (3.7, 5.6) kg/m², =3.21, =0.005), whereas LMI and abdominal-to-gluteal fat percentage ratio did not differ significantly (both >0.05). In the OI overweight or obesity group, both FMI and LMI were lower than in the control group (9.0 (6.1, 9.6) 10.4 (9.2, 11.7) kg/m², 12.1 (11.7, 12.9) 13.9 (13.0, 15.1) kg/m², =3.45,3.72, both <0.05). Overweight or obesity was prevalent among children with OI and was closely associated with fracture frequency. Among those with COL1A1 or COL1A2 variants, the overweight or obese OI children was characterized by higher FMI and lower LMI. - Source: PubMed
Xu X QZheng J PZhou X LLin HChen X FYuan THuang KDong G PChen J SFu J FWu W - Adipose-derived stem cells (ASCs) possess substantial regenerative potential, with lineage fate tightly coupled to metabolic dynamics. In prior work, we found that expression of the tardigrade-derived protein Mitochondrial Abundant Heat Soluble (MAHS) increased stress tolerance and promoted ASC osteogenic differentiation over adipogenic differentiation, although the mechanism remained unclear. Here, we leverage transcriptomic and functional assays to investigate how MAHS expression influences ASC metabolism. Transcriptomic profiling revealed incomplete adipogenic activation alongside upregulation of ossification-associated genes (e.g., ), as well as broader changes in metabolic, Wnt, and YAP signaling pathways consistent with a shift toward osteogenic programming. Functionally, MAHS-expressing ASCs exhibited increased matrix calcium deposition in osteogenic culture, as well as decreased ATP and cAMP levels during basal culture. Further, treatment with forskolin increased cAMP levels, while forskolin or Compound C increased lipid accumulation in MAHS-expressing ASCs. Together, these findings suggest that MAHS expression perturbs metabolic dynamics and signaling networks in ASCs and may contribute to impaired adipogenesis and reinforcement of osteogenic bias. More broadly, this work highlights potential regulatory points that could be targeted to restore lineage balance in metabolically disrupted ASCs. - Source: PubMed
Publication date: 2026/07/15
Rolsma Jordan LLi JulieannaSoh RuthiaNieh ConnorYeh BryceDarch WilliamNieden Nicole ZurMorgan Joshua T - Neonatal calf health critically depends on successful passive transfer of immunity (PTI) via colostrum. Although PTI is routinely assessed by serum IgG concentration, associated systemic proteomic responses remain poorly defined. This study aimed to relate distinct PTI categories to neonatal calf serum proteomes and to evaluate colostrum proteomes to identify proteins influencing passive transfer success. Forty Holstein cow-calf pairs were enrolled. Calf serum was collected before colostrum feeding (0 h) and at 48 h. Serum IgG was quantified by ELISA, and calves were classified at 48 h as failed (FPTI, <1000 mg/dL, n = 11), adequate (APTI, 1000-2000 mg/dL, n = 11), or excellent (EPTI, >2000 mg/dL, n = 18). For proteomic analyses, five calves were purposefully selected in each group; serum samples were pooled by PTI group and time point, and colostrum samples by PTI group, then analyzed by LC-MS/MS in DIA mode. Proteomics identified 54 proteins in colostrum and 80 in serum, 27 of which were shared between matrices. Post-colostrum intake, 21 (FPTI), 5 (APTI), and 30 (EPTI) serum proteins changed significantly from 0 to 48 h. In EPTI calves, haptoglobin (Hp) (51.10-fold) and pigment epithelium-derived factor (PEDF) (27.24-fold) showed the strongest upregulation. At 48 h, lipopolysaccharide-binding protein (LBP) and haemoglobin subunit epsilon (Hb-ε) were lower in EPTI, whereas PEDF, polymeric immunoglobulin receptor (PIGR), and SERPINA3-6 were higher, consistent with coordinated activation of acute-phase, mucosal/transport, and protease-antiprotease pathways. These findings indicate that PTI involves coordinated proteomic changes beyond IgG alone and highlight candidate biomarkers of successful passive immunity in neonatal calves. - Source: PubMed
Publication date: 2026/07/17
Kaçar YiğitTopal OnurMecitoğlu ZaferSağlam NezaketFthenakis George CBatmaz Hasan - At present, colorectal cancer (CRC) ranks as the third most prevalent cancer globally and is the second most common cause of mortality associated with cancer. The expression of A Disintegrin and Metalloproteinase with Thrombospondin Motifs 5 (ADAMTS5) is upregulated in CRC, and high ADAMTS5 expression strongly correlates with an unfavorable prognosis. However, the function of ADAMTS5 in CRC remains unknown. This study aimed to investigate the role of ADAMTS5 in CRC and its potential mechanisms of action. The results showed that ADAMTS5 expression was higher in CRC tissues than in paracancerous tissues. Bioinformatics analysis revealed that its expression gradually increased with tumor progression and that high expression was correlated with poor prognosis. ADAMTS5 knockdown suppressed the proliferation and migration of HCT116 and HT29 cells, and ADAMTS5 inhibition suppressed epithelial-mesenchymal transition (EMT) in HCT116, HT29, and patient-derived organoids. Mechanistic analysis using the STRING database revealed that ADAMTS5 expression was strongly correlated with the Wnt signaling pathway. Western blotting analysis confirmed that ADAMTS5 inhibition suppressed tumor cell invasion and migration and blocked EMT in vitro through the PEDF/Wnt/β-catenin pathway. Furthermore, ADAMTS5 inhibition significantly inhibited tumor proliferation and metastasis within a nude mouse CRC liver metastasis model. These findings indicated that inhibition of ADAMTS5 could suppress CRC progression and metastasis via the PEDF/Wnt/β-catenin pathway. - Source: PubMed
Sun XuanZhang HanqingHu YanJiao ShengyuYao ZeshengYi KeGu GuojianXu Xiaohui - Dry eye disease (DED) is a multifactorial disorder characterized by tear film instability, ocular surface damage, and inflammation. This study investigates the molecular landscape and the therapeutic potential of umbilical cord mesenchymal stem cell-derived extracellular vesicles (UCMSC-EVs) as a standardized, cell-free treatment for DED. UCMSC-EVs were produced from UCMSCs in a scalable 3D bioreactor system and enriched via tangential flow filtration (TFF). The physicochemical properties of UCMSC-EVs were characterized. The molecular cargo of UCMSC-EVs was analyzed via proteomics analysis and microRNA (miRNA) microarray profiling across multiple production lots. Functional potency of UCMSC-EVs was assessed using a human corneal epithelial cell line (HCE-S) wound healing assay and two human inflammation models. UCMSC-EVs exhibited a consistent cargo profile enriched with proteins and miRNAs targeting DED-related pathways, with high proteomic correlation (Pearson's > 0.8) across independent lots. Functional assays demonstrated that UCMSC-EVs promoted HCE-S wound healing and suppressed pro-inflammatory cytokines in a dose-dependent manner. Furthermore, a significant negative correlation was identified between PEDF (Pigment Epithelium-Derived Factor) cargo concentration and interferon gamma (IFN-γ) secretion (Pearson's = -0.724, = 0.005), providing a quantitative link between molecular identity and anti-inflammatory potency. UCMSC-EVs possess favorable physicochemical and molecular characteristics, and demonstrate robust regenerative and anti-inflammatory properties . The high manufacturing consistency and the identification of key molecular drivers, such as PEDF, support the potential of UCMSC-EV as a well-characterized and viable cell-free therapy for mitigating DED progression. - Source: PubMed
Publication date: 2026/06/16
Hsu Qiao-YuHsiung Chia-NiCheng Hsin-HungLien Wen-YuLin Wen-HsienSieber MartinKang Chi-Chih