IL-17A, His, Human Protein
- Known as:
- Interleukin-17A, histidine, Human Protein
- Catalog number:
- z03228-1
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Genscript
- Gene target:
- IL-17A His Human Protein
Related genes to: IL-17A, His, Human Protein
- Gene:
- IL17A NIH gene
- Name:
- interleukin 17A
- Previous symbol:
- CTLA8, IL17
- Synonyms:
- IL-17A, IL-17
- Chromosome:
- 6p12.2
- Locus Type:
- gene with protein product
- Date approved:
- 1993-10-25
- Date modifiied:
- 2019-04-23
Related products to: IL-17A, His, Human Protein
Related articles to: IL-17A, His, Human Protein
- The clinical translation of alloantigen-specific regulatory T cells (AS-Tregs) is constrained by their low frequency in peripheral blood, limited purity, and functional instability following prolonged expansion. To address these hurdles, we developed a strategy to isolate and expand a functional CD25CD27CD70 AS-Treg population. Initially, Tregs were co-cultured with allogeneic dendritic cells in the presence of IL-15, IL-2, and retinoic acid. Proliferating CD25CD27CD70 AS-Tregs were subsequently FACS-sorted and expanded via polyclonal anti-CD3/CD28 stimulation with IL-15, IL-2, rapamycin, and TGF-β. Over three weeks, this protocol yielded a 434-fold expansion, achieving >95% purity (CD25FOXP3) while maintaining a substantial degree (>60%) of -TSDR demethylation, a hallmark of stable Treg lineage commitment. The expanded CD27 AS-Tregs exhibited a robust immunoregulatory phenotype, characterized by high expression of Helios, CTLA-4, and CD39, as well as chemokine receptors associated with allograft and lymphoid tissue homing (CXCR3, CCR4, and CCR7). Functionally, CD27 AS-Tregs suppressed T cell proliferation in an antigen-specific manner, even after exposure to inflammatory cytokines, and showed a concentration-dependent chemotactic response to CXCL10 . In addition, these cells maintained lineage fidelity by lacking the production of inflammatory cytokines such as IFN-γ and IL-17A. Accordingly, transcriptional profiling by RNA sequencing confirmed the enrichment of immunoregulatory signatures and revealed minimal changes in gene expression when expanded Tregs were exposed to inflammatory conditions. Overall, our findings suggest that CD27 AS-Tregs represent promising candidates for more stable, long-term Treg therapy to support transplant tolerance. - Source: PubMed
Publication date: 2026/06/10
Cortés-Hernández ArimelekArteaga-Cruz SaúlMartínez Iturbe Iyari IRosas-Cortina KatyaVigil Mora Marco ALegorreta-Anguiano ErickReyes Barrientos Judith EÁlvarez-Salazar Evelyn KCervera AlejandraSánchez-Hernández Beatriz EDomínguez Armando GamboaSoldevila Gloria - : Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease with systemic features, characterized by painful nodules, abscesses, and sinus tracts. Pro-inflammatory cytokines may serve as biomarkers of disease severity, inflammatory burden, and therapeutic response. The aim of this article is to systematically review the current evidence on serum concentrations of pro-inflammatory cytokines in patients with HS and to evaluate their potential utility as biomarkers of disease activity and personalized treatment guidance. : This systematic review was conducted in accordance with the PRISMA 2020 guidelines. A literature search was performed in PubMed, Embase, and Scopus, with Google Scholar used as a supplementary source. The search included English-language publications from 2015 to 2026. The following keywords were used in combination: "cytokines", "serum", "hidradenitis suppurativa", and "markers". In addition, reference lists and citations of eligible full-text articles were manually screened. Eligible studies for inclusion were peer-reviewed, original human studies reporting serum cytokine levels in patients with HS. Reviews, meta-analyses, case reports, editorials, letters, in vitro and animal studies, conference abstracts without the full text available, non-serum studies, and thematically unrelated publications were excluded. : Database searches identified records in PubMed, Embase, Scopus, and Google Scholar, of which three PubMed studies met the inclusion criteria. Manual screening of reference lists and citations identified three additional eligible publications. Overall, six studies were included in the final qualitative synthesis. The included studies reported elevated serum levels of key inflammatory mediators, including IL-17A, IL-6, IL-1β, IL-23, IL-18, and soluble TNF receptors, in patients with HS compared to healthy controls. Several cytokines were associated with disease severity, and selected markers showed changes during biologic treatment. Stratification into immunological endotypes based on cytokine profiles and clinical features was also proposed. : Pro-inflammatory cytokines, especially those involved in the IL-1β-IL-17 axis, show potential as biomarkers of disease severity and treatment response in HS. Their assessment may support future personalized therapeutic strategies. However, current evidence remains limited by small study numbers and methodological heterogeneity. Further large-scale prospective and longitudinal studies are required before serum cytokine profiling can be implemented in routine clinical practice. - Source: PubMed
Publication date: 2026/06/17
Tekielak AnnaPolak KarinaFrątczak AleksandraBergler-Czop Beata - The glyoxalase pathway detoxifies reactive dicarbonyls generated during hyperglycemia, but the role of its epigenetic regulation in renal dysfunction and inflammatory dysregulation in older adults remains unclear. We investigated CpG-specific DNA methylation within the glyoxal detoxification pathway, focusing on the GLO1 gene, and examined associations with glycemic status, renal function, and systemic inflammation in hospitalized older adults. We identified a single CpG site within the GLO1 gene (cg26053840) significantly associated with fasting glycemia, suggesting that methylation levels at this locus reflects metabolic stress. Higher methylation at cg26053840 was also associated with impaired renal function, including increased serum creatinine and reduced estimated glomerular filtration rate. Additionally, GLO1 methylation correlated with multiple inflammatory indices, including C-reactive protein, erythrocyte sedimentation rate, neutrophil-to-lymphocyte ratio, and the CRP-to-albumin ratio. Associations with circulating cytokines and immune activation markers such as IL-6, IL-17A, GDF-15, CXCL9, CD163, and soluble RAGE further indicated broader immune-metabolic dysregulation. In silico analyses revealed a significant inverse correlation between cg26053840 methylation and GLO1 mRNA expression in the Broad Institute GDAC Firehose dataset. Genomic annotation further identified putative CEBPD and MYF6 transcription factor binding sites in proximity to the CpG site, suggesting a potential regulatory context. These findings support a model in which glycemic dysregulation increases methylglyoxal production, while reduced renal clearance enhances dicarbonyl stress, potentially driving epigenetic modulation of GLO1. These findings suggest the presence of a metabolic-epigenetic-inflammatory axis, although longitudinal and mechanistic studies are required to determine whether it contributes to organ dysfunction and vulnerability in hospitalized older adults. - Source: PubMed
Publication date: 2026/05/29
Fortunato CarloPiacenza FrancescoBadillo Pazmay Gretta VeronicaMalavolta MarcoCardelli MaurizioCherubini AntonioBiscetti LeonardoPelliccioni GiuseppeSoraci LucaGentilini DavideCalzari LucianoMarchegiani FrancescaRecchioni RinaGiordani ChiaraMatacchione GiuliaSbriscia MatildeFantone SoniaGaleazzi RobertaLattanzio FabriziaBonfigli Anna RitaDi Rosa MirkoOlivieri FabiolaGiacconi Robertina - Differentiating inflammatory bowel disease (IBD) from diarrhea-predominant irritable bowel syndrome (IBS-D) remains a major clinical challenge due to overlapping symptoms and the limited specificity of single biomarkers. A reliable, non-invasive multimarker approach is needed to improve diagnostic accuracy and reduce unnecessary endoscopic procedures. To evaluate the diagnostic performance of serum interleukin-17A (IL-17A), neutrophil-to-albumin ratio (NAR), and fecal calprotectin (FCP), individually and in combination, for discriminating IBD from IBS-D and healthy controls in Egyptian patients. In this case-control study, 300 participants (100 with IBD, 100 with IBS-D, and 100 healthy controls) were enrolled. Serum IL-17A, NAR, and FCP were measured, and subgroup analysis was performed for infected and non-infected IBS-D patients. Diagnostic performance was assessed using receiver operating characteristic (ROC) curve analysis, with optimal cutoffs determined by the Youden index. A combined biomarker model was constructed using logistic regression. All biomarkers demonstrated a significant stepwise increase from healthy controls to IBS-D and IBD ( < 0.001). IL-17A, NAR, and FCP were elevated in IBS-D compared with controls, indicating low-grade inflammation, but were highest in IBD. No significant differences were observed between infected and non-infected IBS-D patients. Among individual markers, NAR showed the highest diagnostic accuracy (AUC = 0.923), followed by FCP (AUC = 0.884) and IL-17A (AUC = 0.859). The combined model significantly improved performance (AUC = 0.973), achieving 89% sensitivity and 96% specificity. IBS-D is associated with measurable systemic and intestinal inflammation independent of infection status. The combined biomarker model integrating IL-17A, neutrophil-albumin ratio, and fecal calprotectin demonstrated promising discriminatory performance for differentiating IBD from IBS-D. These findings suggest the potential applicability of combined non-invasive biomarkers in future diagnostic stratification approaches. However, the model was developed and evaluated within a single cohort, and external validation in independent populations is required before future potential clinical application. A multimarker diagnostic panel integrating IL-17A, neutrophil-albumin ratio, and fecal calprotectin demonstrated promising diagnostic performance for differentiating inflammatory bowel disease from IBS-D. The combined model may contribute to future diagnostic stratification strategies in patients with chronic diarrhea. However, these findings were derived from a single cohort and require validation in independent populations before broader clinical application. - Source: PubMed
Publication date: 2026/06/16
Othman GamalEl Sayed Zaki MaysaaElmalki Elmalki NaderBadawy Abdelnaser AAfifi Samir A - Psoriasis is a chronic, immune-mediated inflammatory skin disease for which the development of structurally novel and accessible small-molecule candidates remains of considerable interest. In this study, a series of juglone-derived naphthoquinone analogs was synthesized to explore the influence of substitution pattern on anti-inflammatory activity and cytotoxicity. Their biological profiles were first evaluated in LPS-stimulated HaCaT cells by combining cytotoxicity assessment with nitric oxide (NO) screening. Most derivatives showed reduced cytotoxicity compared with juglone, and preliminary structure-activity relationship analysis indicated that retention of a free hydroxyl group at the C-2 position was generally favorable for both reduction in NO release and cellular safety, whereas C-3 alkyl substitution tended to weaken activity and increase cytotoxicity. Among the tested compounds, compound showed the most favorable balance between reduction in NO release and low cytotoxicity. Further evaluation showed that compound reduced the protein levels of several inflammatory mediators in the culture supernatants of LPS-stimulated HaCaT cells, including TNF-α, IL-6, IL-1β, IL-17A, and IL-23, under the tested conditions. In an imiquimod-induced psoriasis-like mouse model, topical administration of compound partially alleviated IMQ-induced psoriasis-like skin lesions, improved histopathological changes to some extent, and reduced selected inflammatory cytokine levels in serum and skin tissues under the tested conditions. Exploratory target prediction, molecular docking, and in silico ADMET analyses provided supportive computational insight into the biological profile of compound . Overall, these findings suggest that juglone-derived naphthoquinones may serve as useful natural-product-inspired scaffolds for further anti-inflammatory optimization, and compound warrants further investigation in psoriasis-related experimental models. - Source: PubMed
Publication date: 2026/05/29
Bu TongGong ZileMa YudongDai LixiaMa YuchaoYu XiaoyanYang XiaorongMiao XiaolouShang Xiaofei