Human VDBP ELISA kit
- Known as:
- Human VDBP Enzyme-linked immunosorbent assay test reagent
- Catalog number:
- lf-ek0141
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Abfrontier
- Gene target:
- Human VDBP ELISA kit
Related genes to: Human VDBP ELISA kit
- Gene:
- GC NIH gene
- Name:
- GC vitamin D binding protein
- Previous symbol:
- -
- Synonyms:
- DBP, VDBP, hDBP
- Chromosome:
- 4q13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-01-25
Related products to: Human VDBP ELISA kit
Related articles to: Human VDBP ELISA kit
- Piper mollicomum Kunth., a plant traditionally used in rural Brazilian communities since the colonial period, shows promising potential for cancer treatment. This study investigated the phytochemical composition and cytotoxic activity of ethanolic, dichloromethane extracts, and essential oil from the plant's leaves. Chemical analyses, including phytochemical screening, UPLC, and GC-MS, were performed. Cytotoxicity was then assessed using the Sulforhodamine B (SRB) assay on MDA-MB-231 (breast) and A549 (lung) cancer cell lines. The dichloromethane extract showed the strongest cytotoxicity, with IC values of 24.95 and 28.57 µg/mL, respectively. The essential oil exhibited even greater activity (IC = 3.57 µg/mL), with (E)-nerolidol identified as its major component (up to 78.04% in dried leaves). (E)-nerolidol also showed cytotoxicity (IC = 9.04 µM). These findings highlight the potential of P. mollicomum, particularly its essential oil, as a natural source of cytotoxic compounds for breast and lung cancer treatment, reinforcing its ethnopharmacological relevance. - Source: PubMed
Santos Letícia BarbosaMarques Dos Santos Raquel Speziali AroeiraMoreira Nayara CoutoCosenza Gustavo PereiraLeite Paula Mendonçade Souza-Fagundes Elaine Mariade Oliveira Mônica Cristinada Silva Izabella ThaísCastilho Rachel Oliveira - The present study evaluated the anticonvulsant property of hydro-alcoholic leaf extract of Pimenta dioica (L.) Merr. (Allspice), a plant known for its diverse biological properties. Phytochemical screening and ATR-IR spectral analysis confirmed the presence of various metabolites, including flavonoids, tannins, phenolic compounds, and carbohydrates. The acute toxicity study revealed no toxic effects and/or mortality at 2000 mg/kg, suggesting a favorable safety profile of the extract. The extract was administered orally, followed by evaluation in maximal electroshock (MES) and Pentylenetetrazole (PTZ)-induced seizure models. A significant anticonvulsant effect was observed, with 83.3% protection in MES model and notably increasing latency to seizure onset in the PTZ-induced convulsions. GC-MS profiling identified 275 phytocompounds, of which 66 were selected for network pharmacological analysis. This revealed 717 overlapping targets between the selected compounds and epilepsy-related targets. Network pharmacological study suggested AKT1 as a hub gene, and the anticonvulsant effects may be mediated through multiple molecular interactions. Molecular interaction study further supported that the constituents, particularly furaneol, may modulate AKT1 activity through key residue interactions, as observed with the co-crystallized ligand. Overall, the findings support the potential of P. dioica leaf in convulsion, and further work is warranted to explore the active chemicals responsible for the observed activity. - Source: PubMed
Manal DelviSahana Saidarshan R PharnakarSandhya MahadevaswamySoham BhattacharyyaSuman ChakolakusubaMohamed Shabi MNair GouriKarthik Kumar BSundara Saravanan Kamatchi - In this study, lyophilized leaves from olive varieties grown in Turkey were extracted using three different solventswith ultrasonication. Antioxidant capacity, total phenol, phenolic compounds, and enzyme inhibition of the extracts were determined. By LC-HRMS, 27 compounds among 84 different phenolic standards were determined in olive leaves, and approximately 35% of them were oleuropein. Total phenol content varied depending on solvents and variety, and antioxidant capacity varied accordingly. Kilis Yağlık ethanol and Gemlik methanol extracts showed the highest inhibition of α-glucosidase and α-amylase, respectively. The aroma compounds of the leaves were extracted by solid-phase microextraction (SPME) and identified by GC-MS. A total of 52 volatile compounds belonging to seven different classes were identified in olive leaves. Considering the obtained bioactive components and properties, the antidiabetic effect of olive leaves and the volatile compounds in their composition are noteworthy. Overall, this study highlights the valorization of olive leaves as an agro-industrial bio-waste within a circular economy framework, demonstrating how BioE3-driven processing strategies (bioeconomy, bioenergy, and bio-based products) can enable sustainable value addition and resource efficiency. - Source: PubMed
Publication date: 2026/04/15
Koyuncu GulcanKilic Tugba - This study identifies metabolite profiles associated with aroma variations among three Guizhou green teas (Meitan Cuiya, Meitan Maofeng, and Duyun Maojian) using integrated HS-GC-IMS and UPLC-Q-TOF-MSMS analysis. We identified 57 volatile organic compounds and 159 phenolic compounds, with 22 compounds exhibiting relative odor activity values (rOAVs) ≥ 1. A partial least squares regression (PLSR) model was developed to establish quantitative relationships between metabolite concentrations and electronic tongue sensory parameters, demonstrating predictive capability ( = 0.89, = 0.82). Variable importance in projection (VIP) analysis identified heptanal, butanol, and pentanol as major contributors to sensory variance. Significant correlations were observed between specific metabolites and sensory attributes (e.g., heptanal-green perception: = 0.82, = 0.003). Unique metabolite combinations were identified for each tea variety: 2-furanmethanol acetate for Meitan Cuiya, propanol for Meitan Maofeng, and myrcene-D/M for Duyun Maojian. The OPLS-DA model ( = 99.3, = 98.2) provides a classification tool for tea differentiation. Absolute quantification was achieved for 22 key aroma compounds and 15 major phenolics with complete method validation. These findings identify metabolite profiles associated with aroma variations among Guizhou green teas and provide candidate markers for quality assessment. - Source: PubMed
Publication date: 2026/05/18
Zhong Hui-XiongWu Meng-YingLi Fu-XiangChen Xiang-YuJiang Meng-YuanZhang Mei-TingLi An-QiYu Zi-TengLiu WeiCheng Ke-Ke - Early-life vaccinations often yield short-lived antibody (Ab) responses due to limited germinal center (GC) reaction and plasma cell (PC) survival. The aim of the study was to assess how vaccine delivery routes (homologous versus heterologous) in mice shape early-life GC dynamics and outputs. 14 days post-booster immunization, spleen, cervical (CLN), and inguinal (ILN) lymph nodes were analyzed for GC B cells, T follicular helper (T) and regulatory (T) cells, and GC-derived memory B cells and expression of B cell activating factor receptor (BAFF-R)/transmembrane activator and cyclophilin ligand interactor (TACI) on GC and memory B cells. Plasmablast/PC and their B cell maturation antigen (BCMA) expression, vaccine-specific Ab-secreting cells (ASCs), and serum and salivary Abs were also assessed. We observed that the booster route determined the anatomical site of the GC responses. Homologous subcutaneous (s.c.)/s.c. immunization increased GC induction in spleen and s.c. draining LNs, the ILNs, whereas s.c./intranasal (i.n.) immunization primarily induced GCs in mucosal draining LNs, the CLNs. The memory B cell composition was also route-dependent, with s.c./i.n. immunization preferentially generating GC-derived IgM memory B cells across lymphoid tissues. In contrast, homologous s.c./s.c. immunization promoted PC differentiation in spleen, yielding more BCMA cells especially in ILNs and BM, associated with elevated vaccine-specific serum IgG (days 7-14) and increased IgG ASCs in spleen and bone marrow. Heterologous s.c./i.n. immunization instead favored PC differentiation only in the CLNs and elevated serum and salivary IgA Abs. Correspondingly, BAFF-R and TACI expression was elevated on splenic GC B cells following s.c./s.c. immunization, whereas a higher expression was observed on CLNs GC B cells and IgM GC-derived memory cells after s.c./i.n. immunization. Together, these findings demonstrate that the booster immunization route directs the anatomical site of GC activity and determines the dominant GC-derived memory B cell subset. Homologous s.c./s.c. immunization maximizes systemic IgG responses, through enhanced BCMA-associated PC survival, whereas s.c./i.n. immunization promotes IgM GC-derived memory B cell induction while confining PC differentiation and BCMA expression to CLNs, resulting in combined systemic and mucosal Ab responses. These results support rational, route-informed early-life vaccine design to selectively enhance systemic IgG or combined systemic-mucosal antibody responses. - Source: PubMed
Publication date: 2026/05/05
Foroutan Pajoohian PooryaAradottir Pind Audur AnnaMolina Estupiñan Jenny LorenaChristensen DennisPedersen Gabriel KristianOlafsdottir Thorunn AJonsdottir IngileifBjarnarson Stefania P