Mouse pre-microRNA Expression Construct mir-222
- Known as:
- Mouse pre-microRNA Expression Construct mir-222
- Catalog number:
- mmir-222-pa-1
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Sbi systeme bioscience
- Gene target:
- Mouse pre-microRNA Expression Construct mir-222
Related genes to: Mouse pre-microRNA Expression Construct mir-222
- Gene:
- MIR222 NIH gene
- Name:
- microRNA 222
- Previous symbol:
- MIRN222
- Synonyms:
- hsa-mir-222
- Chromosome:
- Xp11.3
- Locus Type:
- RNA, micro
- Date approved:
- 2004-04-23
- Date modifiied:
- 2019-01-30
Related products to: Mouse pre-microRNA Expression Construct mir-222
Related articles to: Mouse pre-microRNA Expression Construct mir-222
- - Source: PubMed
Publication date: 2026/09/26
Zhao XiheWang PingLiu JingZheng JianLiu YunhuiChen JiajiaXue Yixue - Cadmium is a persistent toxic metal that promotes oxidative stress, inflammation, and epigenetic dysregulation; however, the research landscape and recurrent microRNA (miRNA)-mediated responses associated with cadmium exposure remain incompletely characterized. This study integrated bibliometric, structured content, and bioinformatics analyses to identify major research trends and recurrent miRNA signals. PubMed/MEDLINE, Scopus, and Web of Science Core Collection were searched using predefined cadmium- and miRNA-related search terms, yielding 1511 source records. After language/document-type filtering and cross-database deduplication, 817 unique records remained for screening, of which 286 publications formed the final bibliometric corpus. Author-keyword co-occurrence analysis was performed using a minimum occurrence threshold of ≥3, with ≥5 used as a sensitivity analysis. Cadmium and microRNA remained the dominant thematic core, while heavy-metal toxicity, apoptosis, and cadmium-stress responses represented the most recurrent secondary themes. In the final human-relevant biological evidence corpus ( = 32 studies), recurrent miRNAs showed substantial directional heterogeneity rather than a uniformly replicated response: miR-155 was reported in four studies with an equal number of increases and decreases, whereas the let-7 and miR-30 families showed modest downward tendencies, and miR-146b and miR-222 showed modest upward tendencies. These small study counts preclude strong claims of directional reproducibility. The miRNA-target network component should therefore be interpreted as exploratory and hypothesis-generating rather than as evidence of cadmium-specific pathway convergence. Overall, this synthesis supports a broader and more reproducible literature map while emphasizing the need for standardized exposure assessment, miRNA measurement, and independent validation of candidate regulatory signals. - Source: PubMed
Publication date: 2026/09/10
Kesin MuhammetOztan GözdeIşsever Halim - Extracellular vesicles (EVs) are membrane-bound vesicles that regulate intercellular signaling by transporting cellular cargo including RNAs, proteins, and lipids. In recent years, EVs have emerged as promising biologic therapeutics for musculoskeletal repair, recapitulating many of the benefits of mesenchymal stromal cells. However, strategies to enhance the therapeutic potential of EVs remains limited. Here, we investigated how cyclic mechanical strain influences the microRNA (miRNA) cargo and function of EVs produced by primary C57BL/6 murine myogenic cells. Specifically, we aimed to determine how biomechanical strain regimens alter myogenic EV cargo and regulate the transcriptome of recipient myoblasts. We identified miR-222 as a miRNA that was significantly and selectively enriched in low-strain long-duration (LSLD) mechanically strained EVs compared to Static EVs and to high-strain short-duration (HSSD) EVs. Recipient primary myoblasts treated with LSLD EVs displayed distinct transcriptomic changes, characterized by a statistically significant overrepresentation of downregulated predicted target genes associated with miR-222-5p and miR-222-3p at 24 h and 72 h after LSLD EV treatment, respectively. This transcriptomic shift correlated with an increase in Myosin Heavy Chain (MyHC) expression in recipient myoblasts. Together, these findings demonstrate that biomechanical strain regulates the packaging of miRNAs within myogenic EVs, and that LSLD EV delivery is associated with a differentiation-related phenotype in recipient myoblasts. This work provides a foundation for future studies utilizing biomechanical cues to tune EV cargo for potential therapeutic applications in muscle repair and regeneration. - Source: PubMed
Publication date: 2026/09/10
Williams Katherine BChubb LauraOsburn ShelbyZhang QianGuilliams BradleyMeyers MelindaLaRocca Thomas JHamilton Karyn LEhrhart Nicole - Following the publication of the above paper, and an Expression of concern statement (doi: 10.3892/ijo.2025.5808) that was published to draw the readers' attention to the fact that, for the immunohistochemistry images shown in Fig. 6, the 'Control/PCNA' and 'Control/p27kip1' panels appeared to be duplicates of each other, the authors have now responded concerning this issue. After having examined their original data, the authors have realized that this figure was inadvertently assembled incorrectly; specifically, the Control/p27kip1 panel, as shown correctly, was duplicated in the Figure as the Control/PCNA panel. The revised version of Fig. 6, now showing the correct data for the Control/PCNA panel, is shown on the next page. Note that this error did not affect the overall conclusions reported in the study. The authors are grateful to the Editor of for allowing them this opportunity to publish a Corrigendum, and all the authors agree with its publication; furthermore, the authors apologize to the readership for any inconvenience caused. [International Journal of Oncology 36: 913‑920, 2010; DOI: 10.3892/ijo_00000570]. - Source: PubMed
Publication date: 2026/09/04
Zhang JunxiaHan LeiGe YoulinZhou XuanZhang AnlingZhang ChunzhiZhong YueYou YongpingPu PeiyuKang Chunsheng - : Circulating microRNAs (miRNAs) are emerging as accessible biomarkers for multiple sclerosis (MS). In experimental models, miR-221 and miR-222 have been linked to immune and Th17-related pathways, while interleukin-23 (IL-23) is a cytokine driving autoimmune inflammation. This observational study evaluated the expression of circulating miR-221/222 and serum IL-23 concentrations in treatment-naive adult patients with MS. : This prospective, cross-sectional case-control study included 43 untreated adult patients with MS (36 with relapsing-remitting MS and 7 with primary progressive MS) and 37 healthy controls. Demographic features, Expanded Disability Status Scale (EDSS) scores, magnetic resonance imaging involvement, initial symptoms, serum IL-23 concentration, and miR-221/222 expression were recorded. Total RNA, including small RNAs, was isolated; complementary DNA was synthesized by reverse transcription from RNA templates; and reverse transcription-quantitative real-time PCR (RT-qPCR) was performed using RNU6-2 as the endogenous small RNA reference. Individual ΔΔCt values were specified as the primary inferential scale, while 2 fold-change was retained only for descriptive reporting. : The relative expression (2) of miR-221 and miR-222 was higher in patients than in controls. Within the patient group, miR-221 and miR-222 relative expression did not differ by age, sex, EDSS, time since MS diagnosis, MRI involvement area, or initial symptoms. No statistically significant difference in IL-23 levels was observed between the patient and control groups. : The presence of unaltered IL-23 levels alongside significantly elevated miR-221 and miR-222 expressions in treatment-naive MS patients during the remission phase suggests their potential utility as biomarkers for immune regulation. Given the cross-sectional design of this study, no causal or regulatory relationship between miR-221/222 and IL-23 can be inferred. - Source: PubMed
Publication date: 2026/07/29
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