Mouse pre-microRNA Expression Construct mir-216b
- Known as:
- Mouse pre-microRNA Expression Construct mir-216b
- Catalog number:
- mmir-216b-pa-1
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Sbi systeme bioscience
- Gene target:
- Mouse pre-microRNA Expression Construct mir-216b
Related genes to: Mouse pre-microRNA Expression Construct mir-216b
- Gene:
- MIR216B NIH gene
- Name:
- microRNA 216b
- Previous symbol:
- MIRN216B
- Synonyms:
- hsa-mir-216b
- Chromosome:
- 2p16.1
- Locus Type:
- RNA, micro
- Date approved:
- 2007-10-24
- Date modifiied:
- 2019-01-30
Related products to: Mouse pre-microRNA Expression Construct mir-216b
Related articles to: Mouse pre-microRNA Expression Construct mir-216b
- High-grade gliomas (HGGs) are the most aggressive adult brain tumors, with a dismal median survival of approximately 15 months, highlighting the need for novel therapeutic strategies. In a prior immunotherapy trial using dendritic cells against glioblastoma, miR-216b emerged as a potential predictive biomarker. Thus, we hypothesize that miR-216b impacts glioma aggressiveness and thereby therapeutic success. Here, we demonstrate that miR-216b is significantly downregulated in the majority of Isocitrate dehydrogenase 1/2 (IDH) wild-type HGG tissue samples (n = 42) and cell models (n = 18). Functional assays revealed that miR-216b overexpression impairs glioma cell proliferation, migration, and stemness characteristics. Transcriptomic and target prediction analyses identified CDK4, a key cell cycle regulator, as a direct target of miR-216b, confirmed via luciferase reporter assays. Correspondingly, upregulating miR-216b (mimic) via transfection decreased CDK4 mRNA and protein levels accompanied by a p21-dependent increase of cells in G0/G1 phase. In addition, miR-216b expression correlated with increased sensitivity to the CDK4/6 inhibitor Abemaciclib. Notably, miR-216b levels were significantly higher in less aggressive IDH-mutant gliomas (n = 21), linking its downregulation to malignancy grade. Collectively, our findings discovered miR-216b as a tumor suppressor in HGGs, modulating CDK4 expression and affecting the responsiveness to CDK4/6 inhibitors. The observed results support the potential of miR-216b as both a prognostic and predictive indicator in HGGs. - Source: PubMed
Publication date: 2026/04/06
Lang AlexandraBuerger SabineMager LeahErjavc LeaKiesel BarbaraMadlener SibylleJaunecker Carola NadinePacher LindaRobert Finn ChristopherKrausgruber LeonieNascimento AndrezzaMachreich SarahRicken GerdaGabler-Pamer LisaLaemmerer AnnaSpiegl-Kreinecker SabineHackl MatthiasPultar MarianneReisecker JohannesBruckner KatharinaNeudert BarbaraMuellauer LeonhardKirchhofer DominikMischkulnig MarioBerghoff Anna SophieWoehrer AdelheidSenfter DanielRoessler KarlRoetzer-Pejrimovsky ThomasBerger WalterHainfellner Johannes AndreasHoeftberger RomanaWidhalm GeorgErhart FriedrichLötsch-Gojo Daniela - Severe trauma disrupts bone marrow function, triggering physiological changes that impair hematopoiesis, immune cell production, and the bone marrow microenvironment. Bone marrow-derived exosomes play a critical role in intercellular communication, but their contribution to the cellular response to injury remains poorly understood. This study investigates bone marrow exosome microRNA (miRNA) expression in rodents subjected to polytrauma (PT) with and without chronic stress exposure, a model simulating chronic critical illness following injury. - Source: PubMed
Publication date: 2025/11/12
Yoham Athina LPeethala AgnetaBible Letitia EKannan Kolenkode BWheeler CaseyHernández-Ríos MiguelRodhouse Christine EMaile RobertEfron Philip AMohr Alicia M - microRNAs (miRNAs) are known to play critical roles in the regulation of gene expression during neurodegenerative diseases and neurotropic viral infections. However, their specific contribution to the pathogenesis of Powassan virus (POWV) infection in the brain remains poorly understood. Understanding miRNA dynamics in the brain during POWV infection may reveal novel insights into viral neuropathogenesis and host antiviral responses. Therefore, in the present study, we analyzed miRNA expression profiles in the mouse brain at different time points following a peripheral POWV infection. A total of 599 miRNAs were examined at day 3, 6, and 9 post-infection. Infection with POWV resulted in the modulation of several miRNAs in the brain at all time points. There was a progressive increase in the number of dysregulated miRNAs over the course of infection. This correlated with POWV dissemination into the brain with a progressive increase in viral RNA levels that peaked at day 9 post-infection. There was an early upregulation of miR-1983, miR-19a, and miR-216b that persisted until day 9 post-infection. POWV infection also resulted in the downregulation of miR-500 at all examined time points. Using IPA, we determined the significant canonical pathways affected by miRNA dysregulation. POWV infection modulated the activation of the thyroid hormone receptor and retinoid X receptor (TR/RXR) and the regulation of the phosphatase and tensin homolog (PTEN). Additionally, macrophage classical activation and growth arrest and DNA damage-inducible 45 (GADD45) signaling were activated as early as day 3 post-infection and persisted until day 9 post-infection. Furthermore, our analysis revealed the activation of cell death pathways such as necrosis and apoptosis and the inhibition of cell cycle progression, as well as leukopoiesis. To our knowledge, this is the first study to evaluate the modulation of miRNAs in the brain following POWV infection. - Source: PubMed
Publication date: 2025/09/23
Elsharkawy AmanyArora KomalJahantigh Hamid RezaKumar Mukesh - Ovarian cancer (OvCa) is one of the most life-threatening female malignancies that affects 300,000 women annually worldwide. Impaired mechanisms of DNA repair are the leading cause of mutations underlying the OvCa development. microRNAs are short non-coding RNAs that regulate the expression of genes by binding to their transcripts and inducing mRNA degradation or inhibition of translation. Here, we review the miRNA-mediated dysregulation of genes involved in DNA damage response (DDR) and DNA repair pathways in OvCa. Apparently, miRNAs are capable of targeting the crucial mediators of DDR (e.g., miR-203a-3p targeting ATM (Ataxia Telangiectasia Mutated)), homologous repair (such as BRCA1 targeted by miR-9, miR-1255b, miR-193b, and miR-148b), non-homologous end joining (with RNF8 being regulated by miR-214), nucleotide excision repair (involving DDB2 targeted by miR-328-3p), or translesion DNA synthesis (involving RAD18, participating also in homologous repair and targeted by miR-379-5p). We also discuss miRNAs (such as miR-519a-3p, let-7e, miR-216b), which affect responses to OvCa therapy by targeting PARP1 (Poly(ADP-Ribose) Polymerase-1). Finally, we also discuss why, despite the identification of multiple miRNAs capable of regulating DNA repair genes, as well as those involved in the response to therapy, no miRNA-based drugs have been approved for OvCa treatment in clinics. - Source: PubMed
Publication date: 2025/09/15
Arczewska Katarzyna DPiekiełko-Witkowska Agnieszka - Understanding the immune response in fish through transcriptomic and microRNA (miRNA) profiling may unlock critical insights into disease resistance mechanisms. The objective of the present study was to examine the immune modulation of the European seabass (Dicentrarchus labrax) following bacterial infection and vaccination. Therefore, sequencing of circulating miRNA isolated from blood serum and 3'UTR transcriptome sequencing of head kidney was conducted. In the infected fish 19 miRNAs were found to be differentially expressed. This included two novel miRNAs exhibiting high levels in the infected fish. Regarding circulating miRNAs following vaccination, three specific miRNAs have been identified that demonstrated a substantial increase in expression. Two of them, miR-216b and miR-30a-5p, have been documented to possess the capacity to delay the progression of viral infections. 3'UTR sequencing analysis of the infected fish revealed no significant enrichment of down-regulated transcripts. However, there was a significant enrichment of up-regulated transcripts related to ribosome biogenesis and protein processing. In vaccinated fish up-regulated transcripts did not demonstrate substantial enrichment. Down-regulated genes on the other hand were involved in cytoskeleton organization and apoptosis, indicating that cellular disruption might be a potential hindrance to effective immunity. Overall, these results provide first insights into the progression and regulation of host immune responses to pathogen infection and vaccination. Moreover, the detection of in total 13 differential expressed circulating miRNAs, including regulators of critical innate immunity-related genes such as Toll-like receptor 18, suggests a potential for circulating miRNAs to play a significant role in the post-transcriptional control of fish immune defenses. - Source: PubMed
Publication date: 2025/02/24
Sarropoulou ElenaKatharios PantelisKaitetzidou ElisavetScapigliati GiuseppeMiccoli Andrea