Mouse pre-microRNA Expression Construct mir-20b
- Known as:
- Mouse pre-microRNA Expression Construct mir-20b
- Catalog number:
- mmir-20b-pa-1
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Sbi systeme bioscience
- Gene target:
- Mouse pre-microRNA Expression Construct mir-20b
Related genes to: Mouse pre-microRNA Expression Construct mir-20b
- Gene:
- MIR20B NIH gene
- Name:
- microRNA 20b
- Previous symbol:
- MIRN20B
- Synonyms:
- hsa-mir-20b
- Chromosome:
- Xq26.2
- Locus Type:
- RNA, micro
- Date approved:
- 2005-06-30
- Date modifiied:
- 2019-01-24
Related products to: Mouse pre-microRNA Expression Construct mir-20b
Related articles to: Mouse pre-microRNA Expression Construct mir-20b
- Polyendocrine metabolic ovarian syndrome (PMOS), an endocrine disorder with unknown aetiology is the leading cause of anovulatory infertility. N6-methyladenosine (mA), the most abundant internal modification in eukaryotic mRNA, regulates multiple aspects of RNA metabolism and reproductive function. However, the contributions of mA modification and its regulatory factors including microRNAs to the pathophysiology of PMOS remain poorly understood. We therefore investigated global mA abundance, expression of key mA regulatory genes, and selected experimentally validated mA-associated microRNAs in granulosa cells (GCs) from women with PMOS. - Source: PubMed
Publication date: 2026/09/03
Bhingardeve SnehalSagvekar PoojaDesai SadhanaMangoli VijayJagtap RichaMukherjee Srabani - Selenium nanoparticles (SeNPs) have emerged as physicochemically tunable nanoplatforms at the intersection of redox biology and post-transcriptional gene regulation. This review provides a systems-level synthesis of current evidence linking SeNPs to microRNA (miRNA) modulation across cancer, inflammatory, metabolic, and degenerative disorders. A hybrid bibliometric-narrative strategy integrating Scopus, Web of Science, and PubMed initially identified 53 records, of which five met all inclusion criteria and were retained for mechanistic analysis of SeNP-mediated miRNA modulation. Recent studies predominantly employ green and biologically mediated synthesis routes, yielding biocompatible SeNPs with tunable physicochemical properties that determine cellular uptake, redox activity, and gene-regulatory engagement. Mechanistically, current evidence supports the working hypothesis that SeNPs influence redox-sensitive pathways-including Keap1-Nrf2, NF-κB, and p53/SIRT1-to potentially reshape miRNA networks central to oxidative stress, apoptosis, inflammation, and metabolic homeostasis. Key miRNA nodes modulated by SeNPs include miR-16, miR-20b, miR-21, miR-155, and members of the miR-200 family. Functionally, SeNP platforms upregulate tumor-suppressive miRNAs while attenuating oncogenic or pro-inflammatory counterparts, resulting in apoptosis induction, anti-metastatic signaling, mitochondrial restoration, immune modulation, and tissue-regenerative effects in preclinical models. Surface functionalization with polymers, peptides, or aptamers enhances targeting precision and supports integration with biosensing technologies such as surface-enhanced Raman spectroscopy and electrochemical platforms for miRNA detection. Despite promising preclinical evidence, translation remains constrained by synthesis variability, selenium's narrow therapeutic index, incomplete pharmacokinetic-pharmacodynamic mapping, and regulatory ambiguity. Advancing SeNP-miRNA therapeutics will require standardized green manufacturing, quality-by-design frameworks, and multi-omics-guided biomarker integration. Collectively, SeNP-mediated miRNA modulation represents a mechanistically grounded and adaptable platform for precision nanomedicine with emerging theranostic potential. - Source: PubMed
Publication date: 2026/07/24
Moldovan Vlad AndreiCavalu Simona DanielaBaia MonicaLeopold Loredana FlorinaLaslo VasileŚmieszek AgnieszkaVicas Simona Ioana - Oral squamous cell carcinoma (OSCC) is characterized by consistently high mortality rates (≤60%) despite therapeutic advances. This is attributable to diagnostic delays arising from the asymptomatic early stages and time-consuming protocols. Hence, the establishment of reliable biomarkers for the routine assessment of the oral mucosa is imperative. MicroRNAs (miRNAs), key epigenetic regulators of gene expression, represent ideal candidates given their characteristic dysregulation across different pathologies. Here, we aimed to identify novel OSCC-specific miRNAs for the saliva-based detection of OSCC from the presymptomatic stage of early invasion. Through a multistep bioinformatic workflow, four miRNAs (miR-20b-5p, miR-484, miR-185-5p and miR-181d-5p) were identified as disease-specific since they simultaneously regulated >65% of a panel encompassing the 15 primarily overexpressed oncogenes in OSCC and a stage-specific panel including the six upregulated genes that genetically define the malignant stages of sequential oral carcinogenesis. The salivary expression of the identified miRNAs was studied in 31 OSCC patients and 31 healthy controls, using quantitative real-time PCR, followed by statistical analysis and an evaluation of the diagnostic accuracy. All studied miRNAs were significantly downregulated in the saliva of OSCC patients compared to controls (miR-484, < 0.001; miR-181d-5p, < 0.001; miR-185, = 0.008; miR-20b, = 0.026) and exhibited combinatory diagnostic performance of 95.4% ( < 0.001) for OSCC detection. Their expression remained uninfluenced by lifestyle and clinicopathological parameters, including smoking/alcohol, tumor site, grade and disease stage. The proposed 4-miRNA panel exhibits high diagnostic performance for the early, saliva-based detection of OSCC, irrespective of histopathological and lifestyle confounders, highlighting its potential as a robust non-invasive screening tool. - Source: PubMed
Publication date: 2026/06/05
Gintoni IphigeniaVassiliou StavrosKardara Bellou MyrtoBalakas AthanasiosLefantzis NikolaosPapakosta VeronicaVlachakis DimitriosChrousos George PYapijakis Christos - Pneumonia is an acute lung tissue infection that may be induced by various agents, like SARS-CoV-2 virus. The ongoing research focused on the molecular interplay between the lncRNA CASC2 and the miR-200c and miR-20b in tuning SIRT1 and ACE2 levels in Egyptian patients with COVID-19 pneumonia and to examine their correlation with clinical outcomes, inflammatory markers, and prospective role as diagnostic or prognostic biomarkers. One-hundred COVID-19 pneumonia patients, together with thirty healthy controls, were recruited, and blood samples were drawn. The gene expression of CASC2, miR-200c and miR-20b were measured using qRT-PCR, whereas the serum concentrations of SIRT1, ACE-2, and IL-6 were measured using ELISA. The gene expression of CASC2 and miR-20b were downregulated, and miR-200c was upregulated among pneumonic patients relative to healthy control subjects. Moreover, the concentration of SIRT1 and ACE-2 were markedly suppressed, whereas IL-6 was increased among pneumonic patients. CASC2, miR-200c, miR-20b, SIRT1, ACE-2 and IL-6 showed ideal performance as a diagnostic biomarker to discriminate between pneumonia patients and healthy controls. Logistic regression analysis revealed that CASC2, miR-20b, SIRT1, and ACE2 could decrease the odds of pneumonia, while miR-200c and IL-6 could increase the risk. Collectively, the present study explored the potential regulatory role of lncRNA CASC2, miR-200c, and miR-20b in modulating key pneumonia-associated targets, including SIRT1, ACE2, and IL-6. These non-coding RNAs may participate in the inflammatory and immune responses underlying pneumonia pathogenesis. Their particularised expression supports their potential utility as diagnostic or prognostic biomarkers and may introduce new targets for therapeutic modulation. - Source: PubMed
Publication date: 2026/05/24
Ayeldeen GhadaShaker Olfat GAhmed Mona IbrahimAbd-Elmawla Mai A - Human papillomavirus (HPV) is a significant risk factor for head and neck squamous cell carcinoma (HNSCC). HPV positive (HPV) HNSCC is more sensitive to radiotherapy and has a better prognosis than HPV negative (HPV) HNSCC. M1 macrophages not only enhance the radiosensitivity of HPV+ HNSCC, but the M1 macrophages derived exosmoes (M1 exos) also possess anti-tumor activity. However, the role of M1 exos in the radiosensitivity of HNSCC remains unclear. - Source: PubMed
Publication date: 2025/11/27
Liu HuanZhang SiweiLiu ZengchenGong TingdanDuan SiyuLiu TianyangTong FangjiaLi WanlinPan ShuangWei Lanlan