Mouse pre-microRNA Expression Construct mir-200b
- Known as:
- Mouse pre-microRNA Expression Construct mir-200b
- Catalog number:
- mmir-200b-pa-1
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Sbi systeme bioscience
- Gene target:
- Mouse pre-microRNA Expression Construct mir-200b
Related genes to: Mouse pre-microRNA Expression Construct mir-200b
- Gene:
- MIR200B NIH gene
- Name:
- microRNA 200b
- Previous symbol:
- MIRN200B
- Synonyms:
- hsa-mir-200b
- Chromosome:
- 1p36.33
- Locus Type:
- RNA, micro
- Date approved:
- 2004-04-23
- Date modifiied:
- 2019-01-24
Related products to: Mouse pre-microRNA Expression Construct mir-200b
Related articles to: Mouse pre-microRNA Expression Construct mir-200b
- Repeated blood donations deplete 200-250 mg of iron per donation. While biochemical consequences on iron stores are well documented, the role of circulating microRNAs (miRNAs) in maintaining iron homeostasis remains poorly characterized. This study investigated whether repeated blood donation alters iron metabolism-related miRNA expression. - Source: PubMed
Publication date: 2026/08/10
Karaca AzizÇitli ŞenolArpa MedeniSönmez Kübra - Introduction, a key pluripotency gene, plays a central role in breast cancer stem cell (BCSC) biology, driving tumor aggressiveness. Although dysregulation has been widely studied across various cancers, its specific regulatory mechanisms in breast cancer (BC) remain incompletely elucidated. This study examined the regulatory axis of the gene and associated molecular mechanisms underlying BCSC maintenance and prognosis.MethodsThis retrospective cross-sectional study analyzed breast tumor tissues and adjacent normal controls collected consecutively from two tertiary hospitals. promoter methylation was assessed using methylation-Specific PCR, and expression of , , miR-200b-3p, and was quantified by qPCR.ResultsAberrant methylation of the promoter, linked to increased expression, was observed in the BC cohort. Notably, several samples exhibited a poised promoter state, reflecting an epigenetic configuration associated with stemness. Upregulation of in miR-200b-reduced cells was significantly associated with promoter methylation, suggesting a feedback loop modulating methylation dynamics. Additionally, a positive association between deregulated and highlights a positive feedback loop in the dynamic tumor microenvironment. methylation emerged as a strong prognostic marker, characterized by a low hazard ratio and high diagnostic sensitivity. Its combination with improved specificity (SE = 90%, SP = 79%). Reduced and miR200b expression correlated with poor outcomes, and their integration with SOX2 formed a tri-gene signature that enhanced patient stratification. Correlation analyses revealed coordinated expression among , and , supporting their role in methylome regulation.ConclusionThe findings underscore the clinical significance of this axis and its embedded feedback circuits in shaping BC progression and therapeutic response. - Source: PubMed
Publication date: 2026/08/05
Maqbool FarhanAhamed AlfarAnwar SummayaFatima MuqadasZahra ShaneNajeeb ErumSaeed Muhammad - Type 2 diabetes is increasingly recognized as a systemic disorder driven not only by chronic hyperglycemia and insulin resistance, but also by dysregulated interorgan communication. Extracellular vesicles (EVs), including exosomes and microvesicles, have emerged as biologically active carriers of proteins, lipids, and microRNAs capable of modulating gene expression in recipient cells. This narrative review integrates clinical, experimental, and translational evidence on EV-associated microRNAs as candidate biomarkers and potential mediators of diabetic complications, with emphasis on diabetic neuropathy, diabetic kidney disease, diabetic retinopathy, and metabolic dysfunction-associated steatotic liver disease (MASLD). - Source: PubMed
Publication date: 2026/07/16
Ibarra-Salce RaúlDoval-Caballero José Luis EduardoUribe-Cortés DanielEugenio-Ponce Genesis DinoraIbarra-Salce MarielaJaime-Leal OmarGarcía-Sáenz Manuel Ramón - The clinical application of miRNA therapeutics in diabetic foot ulcers (DFUs) is limited by the rapid degradation of miRNAs. We previously reported that topical lipofectamine (Lipo)-based miR-200b-3p delivery by Pluronic F127 (PF127) hydrogel has pro-healing properties in diabetic wounds. However, Lipo's high cost and cytotoxicity concerns impede its clinical use. Chitosan (CS) is an inexpensive biopolymer. We investigated whether the topical delivery of PF127+CS+miR-200b-3p can accelerate wound healing in diabetic mice. - Source: PubMed
Publication date: 2026/06/18
Lo Wan-YuChen Chung-JuSin Cian-HueiHsu Hsun-ChinLi Zhi-XuanWang Huang-Joe - Prostate cancer (PCa) is a common health concern among men, and its early and non-invasive diagnosis is of critical importance. In this study, functionalized polydopamine/poly-L-lysine@urease (PDA/PLL@UR) nanomotors were chemically synthesized and employed for the detection of the prostate cancer-specific urinary biomarker miR-141. The catalytic decomposition of urea by urease enables autonomous propulsion of the nanomotors, enhancing the target recognition efficiency. FAM-labeled ssDNA probes were immobilized on the nanomotor surface specifically hybridized with miR-141, resulting in a significant decrease in fluorescence intensity. To further evaluate the diagnostic performance of the new mobile biosensing system, changes in the speed of nanomotors were monitored under an optical microscope. At the same time, the velocity measurements of the nanomotors were performed in PBS (pH 8) under 808 nm NIR irradiation, and the obtained results were compared with the self-propulsion velocities observed in the urea-containing medium. The nanomotors displayed self-propelled motion in urea solution, reaching an average velocity of 3.665 µm s at 150 nM urea concentration. Calibration curves constructed from fluorescence intensity and speed data exhibited strong linear correlations with high coefficients ( = 0.98 and = 0.99), and the corresponding limits of detection (LoD) were calculated as 7.5 pM and 170 pM, respectively. The specificity of the system was assessed using miR-200b and miR-21 as controls, confirming that the nanomotors selectively responded only to miR-141. Overall, the developed PDA/PLL@UR nanomotors offer a novel nanomotor-based biosensing platform for the rapid, sensitive, and non-invasive detection of PCa biomarkers in urine samples. This approach holds considerable promise for future clinical diagnostic applications. - Source: PubMed
Publication date: 2026/07/09
Özcan Özseven SemanurYurdabak Karaca GözdeÖksüz LütfiWang JosephUygun Öksüz Ayşegül