Mouse pre-microRNA Expression Construct mir-148a
- Known as:
- Mouse pre-microRNA Expression Construct mir-148a
- Catalog number:
- mmir-148a-pa-1
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Sbi systeme bioscience
- Gene target:
- Mouse pre-microRNA Expression Construct mir-148a
Related genes to: Mouse pre-microRNA Expression Construct mir-148a
- Gene:
- MIR148A NIH gene
- Name:
- microRNA 148a
- Previous symbol:
- MIRN148, MIRN148A
- Synonyms:
- hsa-mir-148, hsa-mir-148a
- Chromosome:
- 7p15.2
- Locus Type:
- RNA, micro
- Date approved:
- 2004-04-23
- Date modifiied:
- 2019-01-30
Related products to: Mouse pre-microRNA Expression Construct mir-148a
Related articles to: Mouse pre-microRNA Expression Construct mir-148a
- Gestational diabetes mellitus (GDM) is linked to poor infant metabolic outcomes, potentially via altered human milk (HM) hormones and microRNAs. Since their specific roles remain unclear, this study synthesizes current evidence on HM adipose tissue-derived hormones (particularly adiponectin, leptin, and resistin) and microRNA modifications during gestational diabetes. Firstly, a systematic review following PRISMA 2020 guidelines was conducted (PROSPERO: CRD42024612813). Searches in major databases identified studies comparing HM adiponectin, leptin, or resistin concentrations and/or miRNA profiles between GDM and normoglycemic mothers. Secondly, bioinformatics analysis using miRWalk 3.0, functional enrichment, and network topology mapping examined miRNA interactions with , and genes. Twelve studies were included. Adiponectin showed the most consistent GDM-associated reductions, though findings were context-dependent. Leptin was primarily associated with maternal adiposity rather than GDM status. Resistin evidence was insufficient. Three miRNA studies revealed stage-dependent dysregulation in GDM, with , and linked to infant growth outcomes during the first 6 months. Bioinformatics identified and as targeting all three adipokine genes, with enrichment in glucose homeostasis and insulin resistance pathways. Network analysis highlighted , and as central nodes. GDM is associated with selective alterations in HM adipokines and miRNAs, with adiponectin and specific miRNAs showing the strongest signals. These findings support a conceptual model where GDM shapes HM's molecular composition through interacting endocrine and posttranscriptional mechanisms, potentially influencing infant metabolic programming. Larger longitudinal studies are needed to validate these observations and determine clinical relevance. - Source: PubMed
Publication date: 2026/08/31
Zhang ZhijunDavoudi MaryamGhafourian AmirrezaDehghan ParmidaAhmadi MojdeAyyoubzadeh Seyed MohammadMiao XiaoleiChoobineh HamidAfrisham Reza - Milk-derived extracellular vesicles (EVs) transport microRNAs (miRNAs) that are unusually stable and have been proposed to survive digestion and modulate gene expression in the consumer, although their dietary bioavailability and physiological relevance remain debated. How the predicted regulatory potential of these miRNAs differs among the milks of different animals most relevant to human nutrition has not been systematically compared. Here, we performed an integrative in silico analysis of publicly available small-RNA sequencing data from 29 milk and milk-cell samples of human, cow, goat, and donkey origin. miRNAs were quantified against human (hsa) miRBase references-thereby restricting the analysis to evolutionarily conserved miRNAs with human orthologs-and their predicted effect on the human transcriptome was modeled by integrating predicted (mirDIP database) and experimentally supported (TarBase v9 database) miRNA-target interactions into a per-gene, per-species weighted targeting score. Because miRNAs act predominantly as repressors, this score is read as a prediction of which genes would be post-transcriptionally down-regulated in a recipient. miR-148a-3p dominated the exosomal spectrum of all four species (human, cow, goat, and donkey; ≈21.5% of pooled abundance), and the twenty most abundant miRNAs accounted for roughly three quarters of the signal. Of 4577 robustly targeted genes, a 1809-gene conserved "pan-milk" core showed the highest cross-species targeting and was enriched for transcriptional regulation, PI3K-Akt, MAPK, and TGF-β/SMAD signaling, autophagy and-strikingly-the components of the RNA-interference machinery itself. Species-restricted gene sets recapitulated biologically plausible programs, including a human-biased neuronal/axon-guidance and chromatin module, a donkey-biased transcriptional, epithelial, and immune () module, and a ruminant lipid/cholesterol and insulin-mTOR module. Across categories, we observed a reproducible confidence-exclusivity trade-off. We emphasize that these results are computational predictions that assume dietary miRNA uptake and do not constitute experimental validation. We provide the complete targetome as a hypothesis-generating resource to prioritize candidate genes, pathways, and milk types for future functional, nutritional, and epigenetic investigation. Across the 29 samples from the four species, miRNA composition segregated by species (silhouette width = 0.82, a cluster-separation measure ranging from -1 to 1, with values near 1 indicating well-separated groups) and the category structure exceeded a permutation null, indicating that the between-species signal is robust to differences in dataset origin and milk state. - Source: PubMed
Publication date: 2026/08/02
Zoziuk MaksymDjibagaou Abel DafogoTerrinoni AlessandroKoroliouk DimitriColizzi Vittorio - MicroRNA-148a (miR-148a) has been implicated in various neurological disorders, yet its specific function within the central nervous system remains elusive. In this study, conditional astrocyte-specific miR-148a knockout (cKO) and overexpression (KI) mouse models were established using CRISPR-Cas9 and Cre-loxP technology to elucidate its role in emotional and cognitive regulation. Behavioral analyses demonstrated that miR-148a overexpression induced marked anxiety- and depression-like behaviors, accompanied by astrocyte activation, neuronal ultrastructural damage, and disrupted synaptic morphology. Conversely, miR-148a deletion preserved neuronal integrity, improved synaptic architecture, and produced anxiolytic and antidepressant phenotypes with enhanced cognitive performance. Transcriptomic profiling identified the transcription factor Otx2 as a direct target of miR-148a, mediating downstream modulation of dopaminergic signaling and neurodevelopmental genes. Integrative metabolomic analysis further revealed coordinated alterations in neuroendocrine and neurotransmitter-associated metabolic pathways. Collectively, these findings demonstrate that miR-148a critically modulates astrocyte-neuron interactions and dopaminergic homeostasis through the OTX2 axis, contributing to mood and cognitive disturbances. This work provides mechanistic insight into miR-148a-mediated neuroregulation and highlights its potential as a therapeutic target for affective and cognitive disorders. - Source: PubMed
Publication date: 2026/07/31
Tang YifanFeng HuicongLi YueZhang LupengQu ChiwenZhu TianaiZhang DoudouWen YiZeng XiaominZhang YongPeng Xiaoning - MicroRNAs (miRNAs) are small, non-coding RNA molecules approximately 18-25 nucleotides in length that function as essential regulators in numerous biological processes, including immune system function and inflammatory conditions such as bovine mastitis. They hold considerable potential as biomarkers for this disease. This study aimed to investigate the expression levels of inflammation-associated miRNAs in the milk of cows with clinical and subclinical mastitis. In this study, 15 whole milk samples were collected from dairy cows, comprising three groups: clinical mastitis (N = 5), subclinical mastitis (N = 5), and healthy (N = 5). The expression levels of nine inflammation-related miRNAs (miR-146a, miR-92a, miR-155, miR-383, miR-29B-2, miR-223, miR-148a, miR-200a, and miR-205) were measured and analyzed using real-time quantitative PCR (qPCR). The results revealed a significant upregulation in the expression levels of miR-92a, miR-155, miR-223, and miR-200a in clinical mastitis cases induced by Escherichia coli infection. In contrast, subclinical mastitis caused by coagulase-negativestaphylococci (CoNS) showed a significant downregulation of miR-146a, miR-92a, miR-148a, and miR-205. Furthermore, the expression levels of miR-223 and miR-200a were significantly upregulated in both clinical and subclinical forms of the disease. The findings of this study revealed significant alterations in the expression levels of miR-223 and miR-200a, particularly in clinical mastitis, which highlights greater discriminatory power of these miRNAs as diagnostic biomarkers. Accordingly, milk miRNAs appear to have considerable potential for use as non-invasive biomarkers in the early and differential diagnosis of mastitis. - Source: PubMed
Publication date: 2026/07/29
Khasheii BehnoushMahmoodi PezhmanZahraei Salehi TaghiMohammadzadeh AbdolmajidSadeghi-Nasab AliYavari Morteza - - Source: PubMed
Publication date: 2026/05/19
Zhao WeiZheng JianbaoWei GuangbingYang KuiWang GuanghuiSun Xuejun