Mouse pre-microRNA Expression Construct mir-125a
- Known as:
- Mouse pre-microRNA Expression Construct mir-125a
- Catalog number:
- mmir-125a-pa-1
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Sbi systeme bioscience
- Gene target:
- Mouse pre-microRNA Expression Construct mir-125a
Related genes to: Mouse pre-microRNA Expression Construct mir-125a
- Gene:
- MIR125A NIH gene
- Name:
- microRNA 125a
- Previous symbol:
- MIRN125A
- Synonyms:
- hsa-mir-125a
- Chromosome:
- 19q13.41
- Locus Type:
- RNA, micro
- Date approved:
- 2004-04-23
- Date modifiied:
- 2019-01-24
Related products to: Mouse pre-microRNA Expression Construct mir-125a
Related articles to: Mouse pre-microRNA Expression Construct mir-125a
- Diabetes mellitus (DM) is a chronic metabolic disorder that carries the risk of severe complications, such as cardiovascular disease, renal impairment, and an increased susceptibility to Alzheimer's disease. Considering these significant health implications, it is crucial to deepen our understanding of this condition. MicroRNAs (miRNAs), small non-coding RNAs that regulate gene expression, play a critical role in various biological processes and, when dysregulated, can contribute to the development of diseases such as diabetes mellitus. - Source: PubMed
Publication date: 2026/06/10
Choi Sang AHong Seung-Ho - MicroRNA (miRNA) stands for a class of small, non-coding RNA molecules, typically forming 20 to 25 nucleotides in length, which play a pivotal role in the regulation of gene expression in eukaryotic cells. These molecules are integral to the post-transcriptional regulation of target messenger RNA (mRNA), which they achieve primarily through binding to complementary sequences within the 3' untranslated region (UTR) of the mRNA. miRNAs exert their regulatory effects by binding to their target mRNA, leading to two primary outcomes: mRNA degradation or inhibition of translation. This functionality positions miRNAs as crucial modulators of a variety of biological processes, including cell growth, differentiation, apoptosis, and responses to environmental stress. Furthermore, dysregulation of miRNA expression is associated with pathological conditions, including cancer, cardiovascular disease, and neurological disorders. They are associated with sleep regulation and are helpful in diagnosing diseases, including sleep disturbances. This scoping review aims to summarize existing literature in patients aged 18 to 65 with a main diagnosis of Short Sleep Disorder (SSD), and Insomnia with Short Sleep Duration (ISOSS) and its relationship with miRNAs. The objective of this study is to search for existing evidence on the composition (presence, diversity, and relative abundance) of miRNAs in individuals with and without sleep disturbances, and to know whether the cardiometabolic comorbidities associated with these pathologies imply miRNA alterations. - Source: PubMed
Publication date: 2026/04/02
Perdigoto SusanaMeira E Cruz MiguelRemesal ManuelScala MauroGonzález Soltero María Del RocíoNoriega Clara Azpeletade Pedro Miguel - Bovine mastitis, a major dairy disease, involves inflammation and metabolic dysfunction in mammary epithelial cells (bMECs); however, the regulatory role of microRNAs remains unclear. This study investigated miR-125a in LPS-induced bMECs. miR-125a was downregulated in inflamed bovine mammary tissue and LPS-induced bMECs. Overexpression of miR-125a inhibited pro-inflammatory cytokine release, upregulated proliferation markers, downregulated apoptosis markers, and alleviated oxidative stress. IL6R (interleukin-6 receptor) was identified as a direct target of miR-125a via transcriptome sequencing and dual-luciferase assays. miR-125a suppressed inflammation by targeting , thereby inhibiting NF-κB pathway activation. Rescue experiments showed miR-125a reversed -exacerbated inflammation and restored milk fat synthesis-related gene expression and triglyceride accumulation. In vivo mouse experiments confirmed 's pro-inflammatory and milk fat-suppressing effects. Thus, this study elucidates the miR-125a/IL6R/NF-κB axis in coordinately regulating inflammation and milk fat synthesis in bovine mastitis, offering insights for targeted therapy. - Source: PubMed
Publication date: 2026/04/11
Wang ZhengxingQin MingLi YanqingLi YuhangQi ZhengLi XuxiaoGao JinhuaLuoreng ZhuomaWang Xingping - Clinical responses to mesenchymal stromal cell (MSC) therapies remain variable because MSCs are often treated as uniform biologics despite donor-programmed differences. Evidence indicates that developmental maturity (fetal vs. adult) and biological sex (female vs. male) bias the MSC secretome and downstream signalling (NF-κB, PI3K/AKT-ERK, TGF-β/Smad, Wnt/β-catenin), potentially shaping anti-inflammatory, angiogenic, anti-fibrotic, and regenerative functions. - Source: PubMed
Publication date: 2026/03/24
Azad TasnimHong KimsorWu FeifengMao JueyiZhou XinXie HaotianQiu XinyingLi BinbinZhang LiangTian JidongWen Chuan - MicroRNAs (miRNAs) are key biomarkers for the diagnosis, prevention, and treatment of various diseases. Most miRNAs interact with one another to influence disease occurrence, making multiplex detection essential for miRNA analysis. In this study, we developed a quantitative analysis method for nine miRNAs using a bead-based flow cytometry system. The method was validated using calibration curves, working range, precision, accuracy, specificity, and carry-over assessments in accordance with bioanalysis guidelines. We analyzed the expression of eight inflammation-related miRNAs, including miR-21, miR-24, miR-29a, miR-34a, miR-122, miR-125a, miR-146a, and miR-155, using cel-miR-39 as an internal control. To demonstrate the applicability of the proposed method, we analyzed nine miRNAs in the livers of male and female rats administered CpG ODN 1826 and LPS and compared the results obtained using the RT-qPCR. The results revealed that the calibration standards for the nine miRNAs included nine points, covering a quantification range of 0.20 to 51.20 pg/μL, with a five-parameter logistic (5-PL) curve fit within ±20%. The quality control (QC) samples for all nine miRNAs met the accuracy and precision criteria, with a %RE within ±20% (25% at the LLOQ and ULOQ) and a %CV of ≤20% (25% at the LLOQ and ULOQ). The total error at each QC level was within the acceptance criteria of ≤30% (40% at the LLOQ and ULOQ). Specificity and carry-over evaluations indicated that specific analytes in the multiplex assay were successfully quantified without interference from other analytes, with no carry-over effects observed. Finally, a total of nine miRNAs were quantitatively analyzed in the livers of rats with inflammation induced by CpG ODN 1826 and LPS, compared with the control group. Of these, the expression of miR-21, miR-34a, miR-146a, and miR-155 tended to increase during liver inflammation in both male and female rats. These results were consistent with the RT-qPCR data. In conclusion, this analytical method, validated in accordance with FDA guidelines, enables accurate and quantitative multiplex detection of inflammation-related miRNA biomarkers in liver tissue. It demonstrates strong reliability in current tissue analyses and provides a foundation for developing multiplex miRNA assays capable of analyzing body fluids, with potential applications in future clinical studies. - Source: PubMed
Publication date: 2026/02/12
Lee Eun KyeongJeon Eun-JeongHan So-HyeonJang Eun YoungJung Kyung Jin