Mouse pre-microRNA Expression Construct mir-10a
- Known as:
- Mouse pre-microRNA Expression Construct mir-10a
- Catalog number:
- mmir-10a-pa-1
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Sbi systeme bioscience
- Gene target:
- Mouse pre-microRNA Expression Construct mir-10a
Related genes to: Mouse pre-microRNA Expression Construct mir-10a
- Gene:
- MIR10A NIH gene
- Name:
- microRNA 10a
- Previous symbol:
- MIRN10A
- Synonyms:
- hsa-mir-10a
- Chromosome:
- 17q21.32
- Locus Type:
- RNA, micro
- Date approved:
- 2004-04-23
- Date modifiied:
- 2019-01-24
Related products to: Mouse pre-microRNA Expression Construct mir-10a
Related articles to: Mouse pre-microRNA Expression Construct mir-10a
- Myelodysplastic syndromes (MDS) are a heterogeneous group of disorders caused by abnormalities at the hematopoietic stem cell level and are associated with ineffective hematopoiesis, peripheral cytopenias, and a propensity for leukemic transformation. Recent research elucidates the key mechanisms and roles of ribosomal proteins (RPs), microRNAs (miRNAs), extracellular vesicles (EVs), and long non-coding RNAs (lncRNAs) in the pathophysiology of MDS. Mutations or altered expression of RP genes lead to ribosomal stress and activation of p53, resulting in impaired erythropoiesis and apoptosis of hematopoietic progenitors. Dysregulation of miRNAs modulates gene expression programs that are essential for hematopoietic differentiation and apoptosis. Moreover, EV-associated miRNAs mediate multiple processes, including intercellular communication and regulation of the bone marrow (BM) microenvironment, thereby contributing to DNA damage and clonal evolution. EV-associated miR-10a and miR-15a have been reported to induce DNA damage in HSCs. LncRNAs have emerged as promising tools for cancer diagnosis and prognostic biomarkers. They play critical roles in regulating malignant cell proliferation, apoptosis, and epigenetic modifications. This overview highlights the molecular complexity of MDS pathogenesis, offering insights to support stratified diagnosis and the development of targeted therapies. - Source: PubMed
Publication date: 2026/08/13
Temaj GazmendSaha SarmisthaButtari BrigittaTelkoparan-Akillilar PelinChichiarelli SilviaNuhii NexhibeHadziselimovic RifatSaso Luciano - Here, we present a rapid isothermal strategy for miRNA detection based on ligation-dependent generation of Cas13a-activating RNA and CRISPR/Cas13a-mediated signal amplification. The system employs two DNA probes: a hairpin-structured probe containing a double-stranded T7 promoter within its stem and a second probe encoding a template for Cas13a activator RNA. In the presence of a target miRNA, the probes are ligated to form a functional transcription template, enabling the generation of Cas13a-activating RNA. The resulting RNA activates the Cas13a-crRNA complex, inducing collateral cleavage of a fluorescent reporter for signal generation. The method achieves a limit of detection of 2.56 pM for miR-21 and 11.64 pM for miR-10a, with a linear response over 0-500 pM, while maintaining high specificity with negligible responses to nontarget miRNAs. Importantly, the entire assay is completed within 40 min at 37 °C, highlighting its rapid detection capability. The applicability of the platform is validated using miRNA extracted from HepG2, HeLa, and MCF-7 cells, yielding results consistent with RT-qPCR analysis. Owing to its short assay time, operation at 37 °C, and sequence programmability through probe redesign, this strategy provides a useful framework for miRNA detection. - Source: PubMed
Publication date: 2026/08/04
Lee JiyoonKim JihyunJo Dong YeonLee SuyeonChoi Jung KyoonPark Hyun Gyu - : Cystinuria is an inherited disorder characterized by impaired proximal tubular reabsorption of cystine, resulting in recurrent cystine stone formation. Mutations in the and genes are the main genetic causes of the disease, while variants in have also been associated with cystinuria in specific populations. Despite advances in understanding its molecular basis, cystinuria remains clinically heterogeneous, making disease progression and prognosis difficult to predict. This study aimed to investigate circulating microRNAs (miRNAs) as potential molecular biomarkers, characterize their expression profile in patients with cystinuria, and evaluate their association with clinical indicators of disease severity. : The study group included 16 patients with a clinically established diagnosis of cystinuria, while the control group consisted of 11 healthy individuals without a history of nephrolithiasis. Candidate miRNAs were selected based on their predicted regulatory interactions with genes implicated in cystinuria pathogenesis. Eight microRNAs (miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-1207-3p, miR-10a-3p, miR-3658, miR-141-3p, and miR-200a-3p) were analyzed by quantitative real-time PCR (qPCR). Among the eight miRNAs evaluated, miR-10a, miR-141, miR-200a, and miR-3658 were significantly downregulated in patients with cystinuria compared with healthy controls. Higher expression levels of miR-10a, miR-141, and miR-200a were significantly associated with a greater number of previous surgical procedures ( < 0.05). Additionally, increased miR-10a expression was associated with impaired renal function, defined as serum creatinine levels above 1.2 mg/dL. miR-10a, miR-141, miR-200a, and miR-3658 were significantly downregulated in patients with cystinuria compared with healthy controls. Within the cystinuria cohort, higher expression of miR-10a was associated with impaired renal function, whereas increased expression of miR-10a, miR-141, and miR-200a correlated with a greater number of surgical interventions, suggesting a potential relationship with disease severity. Further studies in larger, genetically characterized cohorts are warranted to clarify the biological role and clinical utility of these miRNAs as biomarkers of cystinuria. - Source: PubMed
Publication date: 2026/07/28
Ayres Daniel CernachPimenta RuanDos Santos Gabriel ArantesCandido PatríciaAntunes MilenaDip Junior Nelson GasparMarchini Giovanni STorricelli Fábio CVicentini Fábio CDanilovic AlexandreBatagello Carlos ALeite Kátia R MNahas William CReis Sabrina TMazzucchi Eduardo - To address the issue of insufficient sensitivity of the chemotherapeutic drug cisplatin in colorectal cancer (CRC), this study aimed to develop a miR-10a antisense oligonucleotide (AMO) system delivered by lipid nanoparticles (LNPs). The aim was to enhance the sensitivity and efficacy of cisplatin by targeting and inhibiting the expression of miR-10a in CRC. - Source: PubMed
Publication date: 2026/07/01
Li ShaorongLi JinzhuaiJiang HongmianZhao KunLuo LifengHuang HualiLiang Zhijie - HDL biogenesis is mainly determined by intestinal and hepatic ABCA1. Inhibiting miR-10b increases macrophage ABCA1 but does not affect intestinal/hepatic ABCA1 or plasma HDL cholesterol in ApoE mice. Given that miR-10a and miR-10b (with similar seed sequences) are comparably expressed in intestine/liver, we hypothesize they redundantly regulate intestinal/hepatic ABCA1 and HDL biogenesis. - Source: PubMed
Publication date: 2026/06/17
Yang YunyanGao ZhuoqiaoXiao QiaolinLian ShaoyanChen JianyinSu WenenZhang YumeiDeng ShaohuiZhao KeHe JiangLiu Chaoqun