MIRSKY FIXATIVE 10X CONC
- Known as:
- MIRSKY FIXATIVE 10X CONC
- Catalog number:
- hs-102-2lt10xcon
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- National diagnostics
- Gene target:
- MIRSKY FIXATIVE 10X CONC
Related genes to: MIRSKY FIXATIVE 10X CONC
- Gene:
- DDX11-AS1 NIH gene
- Name:
- DDX11 antisense RNA 1
- Previous symbol:
- -
- Synonyms:
- CONCR, SCAT4
- Chromosome:
- 12p11.21
- Locus Type:
- RNA, long non-coding
- Date approved:
- 2012-06-30
- Date modifiied:
- 2018-08-03
Related products to: MIRSKY FIXATIVE 10X CONC
(10x) PBS solution without Ca2+, Mg2+(10x) PBS-LOsung ohne Ca2+, Mg2+(10x) PBS_solution without Ca2+, Mg2+0.5 - 10ul pipette tips, extended length, 5 packs per case (10 racks of 96 per pack)0.5 - 10ul pipette tips, extended length, bulk pack of 1000, 20 packs per case0.5 - 10ul pipette tips, extended length, case of 19,200 racked tips (supplied in 20 packs containing 10 x 96 racks)0.5 - 10ul pipette tips, extended length, case of 19,200 racked tips (supplied in 5 packs containing 10 x 96 racks)0.5 - 10ul pipette tips, extended length, case of 20,000 tips (supplied in 20 bulk packs x 1,000)0.5 - 10ul pipette tips, extended length, case of 20,000 tips (supplied in 20 bulk packs x 1,000)0.5 _ 10ul pipette tips, extended length, case of 19,200 racked tips (supplied in 20 packs containing 10 x 96 racks)0.5 _ 10ul pipette tips, extended length, case of 20,000 tips (supplied in 20 bulk packs x 1,000)0.5% Trypsin-EDTA Solution,10X No Phenol Red, Sterile0.5% Trypsin-EDTA Solution,10X No Phenol Red, Sterile0.5% Trypsin-EDTA Solution,10X No Phenol Red, Sterile1,000 Units of Taq DNA Polymerase, Concentration = 5 Units_ul 10X Combination Reaction Buffer Related articles to: MIRSKY FIXATIVE 10X CONC
- Ferroptosis has been increasingly implicated in the progression of various tumors, including hepatocellular carcinoma (HCC). This study aimed to identify ferroptosis-related genes with prognostic significance in HCC. Differential expressed genes (DEGs) and long non-coding RNAs (DElncRNAs) were identified from TCGA LIHC RNA-seq data. A competitive endogenous RNA (ceRNA) network was established based on ferroptosis-related lncRNA-mRNA pairs. Prognostic genes were identified through survival analysis, and selected DEGs were further validated via quantitative PCR. Co-expression analysis identified 218 lncRNA-mRNA pairs, including 216 DEGs and 11 DElncRNAs. Among these, 11 upregulated ferroptosis-related genes were identified as predicted targets of the lncRNA DDX11-AS1. MiR-195 and miR-424 both regulated CDC25A and HELLS. Elevated expression of DDX11-AS1, CDC25A, EZH2, FANCD2, and HELLS was associated with poorer survival. In vitro cellular experiments showed that the knockdown of DDX11-AS1, CDC25A, and HELLS inhibited cell proliferation and invasion by promoting ferroptosis in Huh7 cells. The 11 ferroptosis-related genes and DDX11-AS1 may serve as valuable prognostic biomarkers and potential immunotherapeutic targets in HCC. - Source: PubMed
Publication date: 2026/06/25
Yan KaiHu FangfangTang HaodongZhou Jiahua - Various long noncoding RNAs (lncRNAs) have a crucial part in treating lung adenocarcinoma (LUAD). However, there are few studies on lncRNA DDX11-AS1 in LUAD. We systematically elucidated its potential molecular mechanism in LUAD, which provides a theoretical basis for studying targeted therapy for LUAD. We have analyzed RNA expression in LUAD through TCGA-LUAD data. The binding site (…CAAAUGU…) was predicted by TargetScan database. Associated pathways were predicted by GSEA database. RT-qPCR to analyze RNA level and western blot to analyze protein abundance. Besides, CCK-8, EdU, Transwell and flow cytometry assays were performed to verify how DDX11-AS1, miR-30a-5p and CCNA2 affected LUAD cells. Targeted relationship was verified by dual luciferase reporter and RIP assays. The role of DDX11-AS1 in vivo was tested by a mouse model and immunohistochemistry. Compared with normal lung tissues or cells, LUAD tissues or cells had higher DDX11-AS1 expression and CCNA2 but lower miR-30a-5p expression. Proliferative, invasive and migratory abilities of LUAD cells could be enhanced by DDX11-AS1, which could further regulate cell cycle changes and attenuate apoptosis. MiR-30a-5p could be targeted and down-regulated by DDX11-AS1, which activated cell cycle signaling pathway through miR-30a-5p/CCNA2 axis. DDX11-AS1 promoted tumor growth in mice. Our research demonstrates that DDX11-AS1, through miR-30a-5p/CCNA2 axis, has a cancer-promotive part in LUAD development. It is suggested that DDX11-AS1 may be a new biomarker and therapeutic target in diagnosing and treating LUAD. - Source: PubMed
Publication date: 2026/04/17
Xu ChuanqinAi Bo - Hepatocellular carcinoma (HCC) continues to be a major cause of cancer associated deaths worldwide, highlighting the need for new prognostic biomarkers and treatment strategies. Triaptosis, a recently characterized mode of regulated cell death, has shown potential as a therapeutic target in various malignancies, including HCC. Nevertheless, how long non-coding RNAs (lncRNAs) regulate triaptosis, as well as their function in HCC, is still not well understood. This study integrates bioinformatics and functional validation to delineate the interplay between lncRNAs and triaptosis in HCC progression. Firstly, we confirm that pharmacologically inducing triaptosis, a process centrally mediated by ROS accumulation, with menadione sodium bisulfite (MSB) can inhibit HCC growth both in vitro and in vivo. Furthermore, single-cell RNA sequencing identifies a specific elevation of the triaptosis-related gene in malignant hepatocytes. Through systematic bioinformatics analysis of TCGA data, we develop a 5-lncRNA prognostic signature (, , , , ) with superior predictive power over conventional clinical parameters. Strikingly, functional studies reveal that acts as a crucial oncogenic driver and its depletion suppresses proliferation, migration, and invasion while sensitizing cells to triaptosis via -mediated PI(3)P catabolism. Collectively, our study confirms that triaptosis is a therapeutically targetable signaling in HCC and proposes as a biomarker and therapeutic target, offering new insights into lncRNA-mediated regulation of cell death for precision oncology. - Source: PubMed
Publication date: 2025/11/07
Liu XiaolongZhuang ZiyunCheng JiaxiLi YujieLi DuguangShi ZhaoqiYang JingFan XiaoxiaoLin Hui - Glioma, as the most lethal primary brain malignancy with poor prognosis, requires further elucidation on the functional role of long noncoding RNA (lncRNA) DDX11 antisense RNA 1 (DDX11-AS1) in its pathogenesis, despite its established oncogenic functions in other cancers. Therefore, this study sought to characterize the oncogenic role and molecular mechanism of DDX11-AS1 in glioma. - Source: PubMed
Publication date: 2025/09/26
Wang JianweiYang XinzhiLin LvbiaoYu JianboMao Jie - - Source: PubMed
Publication date: 2025/08/12