TUBAL3 Pre-design Chimera RNAi
- Known as:
- TUBAL3 Pre-design Chimera RNAi
- Catalog number:
- H00079861-R01
- Product Quantity:
- 20 nmol
- Category:
- -
- Supplier:
- Abno
- Gene target:
- TUBAL3 Pre-design Chimera RNAi
Related genes to: TUBAL3 Pre-design Chimera RNAi
- Gene:
- TUBAL3 NIH gene
- Name:
- tubulin alpha like 3
- Previous symbol:
- -
- Synonyms:
- FLJ21665
- Chromosome:
- 10p15.1
- Locus Type:
- gene with protein product
- Date approved:
- 2004-05-27
- Date modifiied:
- 2015-12-11
Related products to: TUBAL3 Pre-design Chimera RNAi
Related articles to: TUBAL3 Pre-design Chimera RNAi
- Epithelial-to-mesenchymal transitions (EMT) require extensive cytoskeletal remodeling to enable changes in cell polarity, adhesion, and migration. Although transcriptional programs controlling EMT are well characterized, how microtubule composition is developmentally regulated during cell state transitions remains poorly understood. Here, we establish a spatially resolved resource delineating the expression of α- and β-tubulin isotypes during neural crest (NC) EMT and tissue differentiation in the chick embryo. Integration of publicly available single-cell RNA sequencing datasets reveals diverse patterns of tubulin gene expression, ranging from broadly expressed isotypes ( and ) to more cell-type-restricted transcripts ( and ). Several tubulin genes, including , , and , are enriched within NC and NC-associated cell types. Using fluorescence in situ hybridization chain reaction (HCR) to spatiotemporally characterize transcripts encoding selected tubulin isotypes, we validate these patterns and map their expression across developmental stages. These transcript patterns provide a map of tubulin gene expression, but additional studies are needed to determine how they relate to microtubule composition. We further show expression of the microtubule motor genes and , revealing overlapping expression patterns between tubulin and motor-associated genes during EMT. Together, these data define a cell state-resolved atlas of tubulin gene expression during vertebrate EMT. - Source: PubMed
Publication date: 2026/07/27
Echeverria Camilo VRamarapu RaneeshBatista Nancy DiazLopez Christian TorresMendez JoanneRogers Crystal D - Neural crest (NC) cells are dynamic embryonic stem cells that undergo an epithelial-to-mesenchymal transition (EMT) and alter their cell states from tightly adherent to migratory and invasive during early development. While EMT transcriptional programs are well characterized, how cytoskeletal architecture is developmentally patterned across EMT states remains poorly understood. Here, we present a spatial and temporal atlas of α- and β-tubulin isotype gene expression during NC EMT in the chick embryo. Single cell RNA-sequencing reveals diversity in tubulin isotype gene expression from ubiquitous (, ) to cell type specific (, ). In addition, we identified novel enrichment of several tubulin isotypes in NC and NC-associated clusters (, , ). Using fluorescent hybridization chain reaction (HCR), we focus on NC EMT and migration states to validate and spatially resolve these expression patterns. Additional characterization in differentiated cells reveals tubulin gene expression in specific neuronal and myogenic populations. We further identify expression of the microtubule motor genes and within neural tube and NC populations, suggesting coordinated regulation of microtubule composition and cargo transport capacity. Together, these data establish that vertebrate NC EMT is accompanied by systematic reprogramming of tubulin gene expression and provide a developmental resource for investigating cytoskeletal control of cell state transitions. - Source: PubMed
Publication date: 2026/03/06
Echeverria Camilo VRamarapu RaneeshBatista Nancy DiazLopez Christian TorresMendez JoanneRogers Crystal D - Infertility is considered a global health issue as it currently affects one in every six couples, with female factors reckoned to contribute to partly or solely 50% of all infertility cases. Over a thousand genes are predicted to be highly expressed in the female reproductive system and around 150 genes in the ovary. However, some of their functions in fertility remain to be elucidated. In this study, 13 ovary and/or oocyte-enriched genes (, , , , , , , , , , , , ) were individually knocked out by the CRISPR/Cas9 system. Mating tests showed that these 13 mutant mouse lines were capable of producing offspring. In addition, we observed the histology section of ovaries and performed in vitro fertilization in five mutant mouse lines. We found no significant anomalies in terms of ovarian development and fertilization ability. In this study, 13 different mutant mouse lines generated by CRISPR/Cas9 genome editing technology revealed that these 13 genes are individually not essential for female fertility in mice. - Source: PubMed
Publication date: 2024/05/08
Pham Anh HoangEmori ChihiroIshikawa-Yamauchi YuTokuhiro KeizoKamoshita MakiFujihara YoshitakaIkawa Masahito - This study aimed to construct a nomogram based on CAF features to predict the cancer-specific survival (CSS) rates of locally advanced rectal cancer (LARC) patients. - Source: PubMed
Publication date: 2024/03/26
Cai HuajunLin YijuanWu YongWang YeLi ShoufengZhang YiyiZhuang JinfuLiu XingGuan Guoxian - Lung cancer is a major cause of cancer-related mortality worldwide, with a 5-year survival rate of approximately 22%. Cisplatin is one of the standard first-line chemotherapeutic agents for non-small cell lung cancer (NSCLC), but its efficacy is often limited by the development of resistance. Despite extensive research on the molecular mechanisms of chemoresistance, the underlying causes remain elusive and complex. - Source: PubMed
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