Rat L-FABP ELISA
- Known as:
- Rat L-FABP Enzyme-linked immunosorbent assay test
- Catalog number:
- kt-433
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Kamiya biomedical company
- Gene target:
- Rat L-FABP ELISA
Related products to: Rat L-FABP ELISA
Related articles to: Rat L-FABP ELISA
- Fat deposition determines beef marbling grade and meat quality, but the underlying molecular mechanisms remain unclear. This study aimed to investigate the role of bovine PPARD in lipid deposition, especially its effect on the expression of fatty acid transport genes (, , and ) and the lipid droplet-associated gene () in liver tissues from cattle with different marbling grades. The mRNA abundance and protein levels in liver tissues from thirty-one Wagyu × Angus crossbred beef cattle (25-26 months old) with different marbling grades (according to GB/T 29392-2022, based on the marbling richness of the longissimus dorsi muscle at the 12th-13th rib interface) were analyzed by RT-qPCR and Western blot, respectively. Additionally, was knocked down and overexpressed in bovine mammary epithelial cells to validate its effects on lipid-metabolism-related genes. The results showed that the mRNA levels of , , , , , and were significantly higher ( < 0.01) in livers tissues from the A3 and A4 groups (high marbling) than in those from the A1 and A2 groups (low to moderate marbling). Western blot analysis revealed significantly higher PPARD protein expression in the A3 and A4 groups (high marbling) than that in the A1 and A2 groups (low to moderate marbling) ( < 0.05). It should be noted that the sample size of Group A4 is only 2, and the results of this group should be considered as a preliminary trend that needs to be validated with larger sample sizes. Cellular experiments confirmed that knockdown significantly decreased mRNA expressions of , , and ( < 0.01), while overexpression significantly increased their mRNA levels ( < 0.05). These results indicate a positive correlation between PPARD expression and the transcriptional levels of genes involved in fatty acid transport and lipid droplet storage, suggesting that PPARD may be associated with hepatic lipid metabolism and potentially contribute to marbling development. These findings suggest that the PPARD signaling pathway contributes to hepatic lipid deposition and may play a role in marbling formation in beef cattle. - Source: PubMed
Publication date: 2026/07/06
Wang KaiyouWang QiWang QinyuTian ShuaiyingQi YingZhang LinXing BaokuiTuliguer Li Qiuling - The liver is the central organ for metabolism and detoxification, and its function gradually declines with age, often accompanied by pathological changes such as lipid metabolism disorders, inflammatory responses, and fibrosis, significantly increasing the risk of metabolic dysfunction-associated steatotic liver disease (MASLD) and metabolic dysfunction-associated steatohepatitis (MASH). Ginsenoside Rb1 (Rb1) is a major active constituent of ginseng. However, its regulatory mechanism on hepatic lipid metabolism and MASLD/MASH-related phenotypes during natural aging remains unclear. - Source: PubMed
Publication date: 2026/04/02
Wu LinSi QiZhou BinChe YanyunLiu YongZhao YingyingZhang XimeiShi GuangyaoHao BaoshunYu ShujieQian XiaoxianZheng JianruiLiu Dinghui - Early detection and effective monitoring of gastrointestinal injury are needed for efficient medical management of the radiation-exposed victims. This study evaluated fatty acid-binding proteins (FABP1, FABP2, and FABP6) in combination to accurately predict and monitor radiation-induced gastrointestinal injury in C57Bl/6 mice. The response of FABPs was checked upon exposure to a range (2.5 Gy-20 Gy) of gamma radiation doses and their availability in serum from 2 h to 7 d. These alterations were correlated with structural changes in the intestine and the expression of genes encoding tight junction proteins, Zona occludens-1 and Occludin. Tissue-specific expression was also checked. FABP2 expression was found abundant and specific to the intestine, where it co-localized with FABP1 and FABP6. An increase in serum FABPs was noted in a dose- and time-specific manner, which correlated with radiation-mediated intestinal injury and downregulation of Zona occludens-1 and Occludin. FABP1, FABP2, and FABP6 were found to be upregulated at 9 Gy and above and were available in serum within 24 h of exposure. FABP1 and FABP2 were detected in serum between 24 h and 72 h, whereas FABP6 had a prolonged time window (24 h to 5 d). In serum, FABP1, FABP2, and FABP6 peaked at 24 h, 48 h and 72 h, respectively, suggesting that the three FABPs in combination can detect GI injury as early as 24 h. These proteins displayed reliable specificity based on ROC curve construction. Together, FABP1, FABP2, and FABP6 could detect and assess the progression of radiation-induced gastrointestinal injury rapidly, which may facilitate in managing exposed individuals following radiological emergencies. - Source: PubMed
Publication date: 2026/06/23
Sharma SuchitraRehan AlizaDutta Ajaswrata - Diabetic kidney disease (DKD) is a chronic microvascular complication of diabetes mellitus and a leading cause of chronic kidney disease worldwide. Microalbuminuria remains the current gold standard for the diagnosis and staging of DKD; however, it often reflects established renal injury rather than early pathological changes. Fatty acid-binding protein 1 (FABP1), a cytoplasmic protein abundantly expressed in the renal proximal tubules, is released into the urine in response to tubular damage. FABP1 plays an essential role in intracellular fatty acid transport and metabolism and may serve as a potential biomarker for tubular involvement in pediatric diabetic kidney disease. A case-control study was performed on 90 children (30 T1DM with DKD, 30 T1DM without DKD [non-DKD], and 30 controls). Albumin-creatinine ratio (ACR) and serum FABP1 were ELISA measured. FABP1 was significantly higher in the DKD group versus non-DKD (p = 0.026) and controls (p = 0.009). FABP1 positively correlated with ACR (r = 0.25, p = 0.018), total cholesterol (r = 0.25, p = 0.019), and LDL-C (r = 0.25, p = 0.017). A cutoff > 189 ng/L discriminated DKD from non-DKD (AUC = 0.67, sensitivity 63%, specificity 73%).Conclusion: Serum FABP1 may represent a complementary biomarker of tubular involvement in pediatric diabetic kidney disease. - Source: PubMed
Publication date: 2026/06/16
Aboelenin Hadil MohamedElTaher HebaElzehery RashaNosir Sara MahmoudAl-Haggar Mohammad - Chemical pollution is a global threat to human health, yet the toxicity mechanisms of most contaminants remains unknown. Here, we applied an ultrahigh-throughput affinity selection-mass spectrometry (AS-MS) platform to systematically identify protein targets of prioritized chemical contaminants. After benchmarking the platform, we screened 50 human proteins against 481 prioritized chemicals, including 446 ToxCast chemicals and 35 per- and polyfluoroalkyl substances (PFAS). Among 24,050 interactions assessed, we discovered 35 interactions involving 13 proteins, with fatty acid-binding proteins (FABPs) emerging as the most ligandable protein family. Given this, we selected FABPs for further validation, which revealed a distinct PFAS binding pattern: legacy PFAS selectively bound to FABP1, whereas replacement compounds, perfluoroether carboxylic acids, unexpectedly interacted with all FABPs. X-ray crystallography further revealed that the ether group enhances the molecular flexibility of alternative PFAS to accommodate the binding pockets of FABPs. Our findings demonstrate that AS-MS is a robust platform for the discovery of protein targets beyond the scope of ToxCast and highlight the broader protein-binding spectrum of alternative PFAS as potential regrettable substitutes. - Source: PubMed
Publication date: 2026/06/05
Yang DiwenWang XiaoyunLiu JiabaoGong YufengNair PranavSun JianxianQian XingCui JingyuanZeng HongDong AipingHarding Rachel JBurgess-Brown NicolaBeyett Tyler SSong DatongKrause Henry MDiamond Miriam LBolhuis Derek LBrown Nicholas GArrowsmith Cheryl HEdwards Aled MHalabelian LevonPeng Hui