Rat L-FABP ELISA
- Known as:
- Rat L-FABP Enzyme-linked immunosorbent assay test
- Catalog number:
- kt-433
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Kamiya biomedical company
- Gene target:
- Rat L-FABP ELISA
Related products to: Rat L-FABP ELISA
Related articles to: Rat L-FABP ELISA
- Broodiness is a natural behavior whereby female birds stop laying eggs to sit on and hatch them. This behavior is regulated through genetic, hormonal, and environmental factors. The Taihe Black-Boned Silky Fowl (TBSF), a Chinese traditional domestic breed, exhibits a strong brooding tendency; however, the molecular mechanisms underlying this trait remain unclear. In this study, we performed an integrated multi-omics analysis to characterize differences between two groups of TBSF hens: 8 individuals undergoing 30 days of active brooding (BR30) and 8 individuals in the normal laying egg stage (NB), selected from a total group of 230 hens. We combined 16S rRNA sequencing, untargeted metabolomics, and hepatic transcriptome sequencing, with statistical analyses including QIIME 1.9.1, OPLS-DA (VIP > 1), Student's -test ( ≤ 0.05), and DESeq2 (|logFC| ≥ 1, FDR < 0.05) for differentially expressed genes (DEGs), respectively, and Pearson's correlation analysis for multi-omics integration. Phenotypically, brooder hens showed significantly reduced feed intake, body weight, and main digestive tissue indices, alongside altered liver and blood biochemical parameters. Hepatic transcriptome analysis identified 1582 DEGs between groups, enriched in pathways related to fatty acid oxidation and the amino acid degradation pathway. In addition, 16S rRNA sequencing revealed distinct gut microbial community structures: the NB group was enriched in and , while the BR30 group was enriched in and . Metabolomic profiling identified a total of 143 differential metabolites, which were enriched in lipid and amino acid metabolites, including alpha-linolenic acid and pyruvate metabolites. Multi-omics correlation analysis revealed tight associations between gut microbial taxa, circulating metabolites, and hepatic gene expression. Specifically, beneficial lipid metabolites, including phospholipids, lysophosphatidylcholines, and sphingomyelins, were positively correlated with and the , as well as with key hepatic lipid metabolism genes , , and . In summary, this study reveals that the gut-liver axis plays a critical part in the modulation of energy metabolism during broodiness, and further highlights new insights into metabolic targets that could optimize reproductive behavior and enhance poultry production. - Source: PubMed
Publication date: 2026/08/05
Haji Ramlat AliHu JingRuan JimingLiang HaipingWan ZiyueOmar Salma MbaroukWei QingXie XianhuaHuang YanmingCao JiHuang Jianzhen - Chronic fatty acid (FA) elevation drives metabolic dysfunction-associated liver disease (MASLD). Hepatocytes buffer transient lipid overload via coordinated adaptive mechanisms, but the temporal limits of this capacity remain undefined. - Source: PubMed
Publication date: 2026/08/07
Serra IlariaBisconti ElisaVari FrancescoStanca EleonoraDe Castro FedericaAngilè FedericaVergara DanieleFanizzi Francesco PaoloGiudetti Anna M - Metabolic dysfunction-associated steatotic liver disease (MASLD) is a heterogeneous condition where overlapping cardiometabolic risk factors challenge accurate prognostic prediction. - Source: PubMed
Publication date: 2026/07/29
Diambra LuisSookoian SilviaPirola Carlos Jose - Estrogen deficiency during menopause may impair gut barrier integrity, increasing permeability and leading to low-grade inflammation. This study aims to assess blood I-FABP levels as a biomarker of gut barrier integrity in menopausal women and to examine their association with menopausal symptoms and lifestyle factors. - Source: PubMed
Keskinoğlu KezbanMete BurakÇakir Güngör Ayşe Nur - Fat deposition determines beef marbling grade and meat quality, but the underlying molecular mechanisms remain unclear. This study aimed to investigate the role of bovine PPARD in lipid deposition, especially its effect on the expression of fatty acid transport genes (, , and ) and the lipid droplet-associated gene () in liver tissues from cattle with different marbling grades. The mRNA abundance and protein levels in liver tissues from thirty-one Wagyu × Angus crossbred beef cattle (25-26 months old) with different marbling grades (according to GB/T 29392-2022, based on the marbling richness of the longissimus dorsi muscle at the 12th-13th rib interface) were analyzed by RT-qPCR and Western blot, respectively. Additionally, was knocked down and overexpressed in bovine mammary epithelial cells to validate its effects on lipid-metabolism-related genes. The results showed that the mRNA levels of , , , , , and were significantly higher ( < 0.01) in livers tissues from the A3 and A4 groups (high marbling) than in those from the A1 and A2 groups (low to moderate marbling). Western blot analysis revealed significantly higher PPARD protein expression in the A3 and A4 groups (high marbling) than that in the A1 and A2 groups (low to moderate marbling) ( < 0.05). It should be noted that the sample size of Group A4 is only 2, and the results of this group should be considered as a preliminary trend that needs to be validated with larger sample sizes. Cellular experiments confirmed that knockdown significantly decreased mRNA expressions of , , and ( < 0.01), while overexpression significantly increased their mRNA levels ( < 0.05). These results indicate a positive correlation between PPARD expression and the transcriptional levels of genes involved in fatty acid transport and lipid droplet storage, suggesting that PPARD may be associated with hepatic lipid metabolism and potentially contribute to marbling development. These findings suggest that the PPARD signaling pathway contributes to hepatic lipid deposition and may play a role in marbling formation in beef cattle. - Source: PubMed
Publication date: 2026/07/06
Wang KaiyouWang QiWang QinyuTian ShuaiyingQi YingZhang LinXing BaokuiTuliguer Li Qiuling