Polyclonal DEDAF
- Known as:
- Polyclonal DEDAF
- Catalog number:
- pc-106
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Kamiya biomedical company
- Gene target:
- Polyclonal DEDAF
Related genes to: Polyclonal DEDAF
- Gene:
- RYBP NIH gene
- Name:
- RING1 and YY1 binding protein
- Previous symbol:
- -
- Synonyms:
- YEAF1, AAP1, DEDAF
- Chromosome:
- 3p13
- Locus Type:
- gene with protein product
- Date approved:
- 2000-01-28
- Date modifiied:
- 2016-10-05
Related products to: Polyclonal DEDAF
Related articles to: Polyclonal DEDAF
- - Source: PubMed
Publication date: 2026/06/08
Cieślikowska AgataGawlik MarzenaFranaszczyk MariaCiara ElżbietaPłoski RafałIwanowski PiotrJurkiewicz ElżbietaMadej-Pilarczyk Agnieszka - The developing limb is a classical model system for studying genetic aspects of pattern formation and mechanisms underlying tissue differentiation. The transition from early to differentiated cell fates during limb bud development requires the precise spatial and temporal regulation of gene activation and silencing programs. However, how transcriptional silencing of early genes is achieved remains poorly understood. Here, we investigate the role of the Polycomb Repressive Complexes (PRC1 and PRC2) in this transcriptional transition in the developing mouse forelimb. We show that the progression from early (E10) to late (E12.5 distal) forelimb stages is associated with shifts in promoter-proximal SUZ12 occupancy at early developmental genes. Conditional inactivation of results in derepression and ectopic distal expression of early genes, which occurs despite the persistence of RING1B and H2AK119Ub. We further identified that SUZ12/RING1B co-occupied targets were also bound by RYBP-containing variant PRC1, indicating parallel recruitment of canonical and variant PRC1. Functional analyses of the combined inactivation of EED and RING1B reveal severe limb malformations and significant gene derepression, exceeding the effects of single EED or RING1B inactivation. Together, our findings demonstrate that the functional convergence of canonical and variant PRC1 at key developmental loci is required to maintain robust transcriptional silencing and regulate transitions in gene expression programs required for proper limb morphogenesis. - Source: PubMed
Publication date: 2026/06/01
Gentile ClaudiaPaul RachelGuerard-Millet FannyVilliard GabrielPoitras ChristianMayran AlexandreRobert FrançoisKmita Marie - Selective gene expression is pivotal in orchestrating human development. Specifically, trithorax group (TrxG) and polycomb group (PcG) components play crucial roles in transcriptional activation and repression of state-specific stem cell expression programs, yet the mechanisms underlying their selective genomic binding remain poorly understood. In this study, we report that the polycomb repressive complex 1 (PRC1) subunit RYBP co-localizes with TrxG component WDR5 and selectively enriches PcG component RING1B in condensates in murine embryonic stem cells (ESCs). RYBP deficiency impairs the genomic binding of WDR5 and RING1B. Further, STAT3 excludes RING1B binding at RYBP-associated transcriptionally active loci. Additionally, RYBP depletion attenuates WDR5-dependent activation of DNA repair gene expression and facilitates the transition of ESCs to 2-cell-like cells. Finally, RYBP depletion disrupts RING1B deposition at lineage-specific genes, promoting ESC differentiation towards mesendoderm fate. These findings uncover RYBP as a regulator of selective genomic binding of TrxG and PcG components, providing insights into their roles in cell fate determination during development. - Source: PubMed
Publication date: 2026/04/28
Wei ChaoSun JunLiu ZhuoyanWang MulanTan JinHuang XiaonaDai RanranSu KangYang ShiwenChen Tara S RTian QiTang XiuxiaoTian XiaolinHuang Dong-FengBai JinXiao XueShen XiaotingXia JuanDing JunjunFan Lili - Mono-ubiquitination of histone H2A at lysine 119 (H2AK119Ub) is deposited by the Polycomb repressive complex 1 (PRC1) and represents an abundant post-translational modification (PTM) of histones. H2AK119Ub is crucially involved in the regulation of a wide range of biological processes, including organization of the genome into distinct functional domains, gene silencing, and the maintenance of cell identities during development, among others. Biochemically, the deposition and removal of H2AK119Ub are tightly controlled in cells owing to a dynamic balance between the specific "writers" (i.e., PRC1) and "erasers" (i.e., deubiquitinases (DUBs) such as BAP1 and USP16). Furthermore, the increasing evidence establishes a notion that H2AK119Ub serves as a signal for recruiting specific "readers" (such as JARID2, DNMT3A, RYBP, SSX, and RSF1), which elicit the critical downstream effects such as modulating gene transcription, maintaining genome integrity, and shaping cell identity. This H2AK119Ub-based signaling is often perturbed in human diseases, pointing to a connection between its dysregulation and pathological development. This review is aimed at providing a timely, in-depth analysis of the molecular machinery governing H2AK119Ub, its interactions with other chromatin factors, and its causal role in the onset and progression of diseases, notably cancer. - Source: PubMed
Publication date: 2026/04/01
Wu DamuZhong HaiqingCai LingWang Gang Greg - Genome sequencing (GS) was applied to 3317 individuals from 1452 Korean families with suspected rare genetic disorders to assess diagnostic yield and clinical utility. Patients were categorized into 16 clinical subgroups with curated phenotypes, and variant interpretation was refined by post-analytic phenotype matching. A molecular diagnosis was achieved in 46.2% of families, influencing clinical management in 18.5% of diagnosed cases. Family-based GS had a higher yield than singleton testing (48.5% vs. 41.5%). Neuromuscular and neurodevelopmental disorders showed the highest yields. GS-specific variant types, including deep intronic, noncoding, complex structural variants, and tandem repeat expansions, accounted for 14.6% of diagnoses. Secondary findings were identified in 4.3% of individuals. Novel disease-associated genes such as RYBP, DNAJA3, CAMK2D, and small nuclear RNA genes were also reported. These results highlight the diagnostic power of GS and support its use as a first-tier test, especially in underrepresented populations. - Source: PubMed
Publication date: 2025/12/08
Lee SeungbokSeo Go HunKim Soo YeonJang Se SongJang SeoyunChoi SongjiChin HyungjinLee Seung JaeOh Dong EonRyu Seung WooKim JihyeMoon DongseokJang SeokhuiLim Byung ChanMoon JangsupHan HeonjongLee HaneChae Jong-Hee