DA-7 Keratin 18
- Known as:
- DA-7 Keratin 18
- Catalog number:
- mc-969
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Kamiya biomedical company
- Gene target:
- DA-7 Keratin 18
Related genes to: DA-7 Keratin 18
- Gene:
- DNAAF1 NIH gene
- Name:
- dynein axonemal assembly factor 1
- Previous symbol:
- LRRC50
- Synonyms:
- FLJ25330, ODA7, CILD13, swt
- Chromosome:
- 16q24.1
- Locus Type:
- gene with protein product
- Date approved:
- 2005-05-26
- Date modifiied:
- 2016-09-30
Related products to: DA-7 Keratin 18
1C7 Keratin 1356 kDa cytokeratin,CK-10,Cytokeratin-10,K10,Keratin, type I cytoskeletal 10,Keratin, type I cytoskeletal 59 kDa,Keratin-10,Krt10,Krt1-10,Mouse,Mus musculus67 kDa cytokeratin,CK-1,Cytokeratin-1,Hair alpha protein,Homo sapiens,Human,K1,Keratin, type II cytoskeletal 1,Keratin-1,KRT1,KRTA,Type-II keratin Kb167 kDa cytokeratin,CK-1,Cytokeratin-1,K1,Keratin, type II cytoskeletal 1,Keratin-1,Krt1,Krt2-1,Mouse,Mus musculus,Type-II keratin Kb16B10 Keratin 480 Keratin, Panacidic Cytokeratin prediluted - Mouse monoclonal [AE1] to acidic Cytokeratin prediluted; Cytokeratin-9; CK-9; Keratin-9; K9 MonoclonalAcidic hair keratin K31 antibodyAcidic hair keratin K31 antibody, Polyclonal Antibodies, Host Guinea PigAcidic hair keratin K32 antibodyAcidic hair keratin K32 antibody, Polyclonal Antibodies, Host Guinea PigAcidic hair keratin K33 antibodyAcidic hair keratin K33 antibody, Polyclonal Antibodies, Host Guinea PigAcidic hair keratin K34 antibodyAcidic hair keratin K34 antibody, Polyclonal Antibodies, Host Guinea Pig Related articles to: DA-7 Keratin 18
- Peripheral blood mononuclear cells (PBMCs) represent a critical component of the immune system, orchestrating both cellular and humoral responses while maintaining immunological homeostasis. This study investigates the PBMC immune response to Pasteurella multocida (P. multocida) infection in goats using single-cell RNA sequencing (scRNA-seq). PBMCs from goats before and after P. multocida infection were used for scRNA-seq), and pathological examinations were performed on different tissues of the infected hosts. Results demonstrated that recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) detected P. multocida in peripheral blood and lungs, with histopathology confirming lesions. A total of 42 615 cells were annotated as nine cell subgroups, revealed dynamic alterations in immune cell populations, including significant increase in B cells and monocytes alongside decreased T cells and megakaryocytes post-infection. Further analysis identified 48 differentially expressed genes consistently modulated across comparison groups, with significant enrichment in immune-related Gene Ontology (GO) terms, as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including IL-17 signaling pathway and cytokine-cytokine receptor interactions. Pseudotime trajectory uncovered four B cell and two T cell differentiation lineages. Inter-cell communication exhibited enhanced activity post-infection. Integration with RNA-seq data revealed key genes (IL1R2, RBM34, EDEM3, and MZB1) potentially critical in early infection. These findings substantially advance the understanding of PBMCs-mediated immune responses during the early stage of P. multocida infection in goats, identifying key genes and immune pathways. The study not only provides valuable insights into goat immunology but also establishes a foundation for developing improved diagnostic tools and targeted therapeutic interventions against this economically significant pathogen. - Source: PubMed
Publication date: 2026/05/05
An QiChen TaoyuJiang JunmingWu HuiWu GuanshengJiao ZizhuoLi ShiyuanMeng YongTang JiayangChen YuanyuanPan HaojuLi HongZhang ZhenxingCheng YiwenFu YujingHuang ZijingManchu RigaChen QiaolingWang Fengyang - Immunoglobulin A (IgA) is the most abundantly produced antibody in mammals, with its secretory dimeric form playing a central role in maintaining intestinal homeostasis. Although the J chain is known to be essential for IgA transcytosis, its precise role in IgA biosynthesis and secretion is not fully understood. Here, using CRISPR/Cas9-edited J558 plasmacytoma cells, a mouse line that secretes IgA, we demonstrate that MZB1 and the J chain act sequentially to ensure proper IgA assembly. MZB1 stabilizes α-heavy chain-light chain complexes (HL complexes), thereby enabling their efficient association with the J chain, which subsequently drives rapid assembly into IgA dimers and higher-order polymers. Loss of MZB1 reduced the secretion of dimeric IgA, whereas J chain deficiency caused both excessive intracellular accumulation and secretion of HL complexes, while allowing the generation and secretion of limited amounts of monomeric IgA but completely abolishing dimer formation. Combined deficiency reproduced the additive defects of the single knockouts, confirming their cooperative function in a shared assembly pathway. , loss of MZB1, the J chain, or both altered IgA abundance and form, differentially affected susceptibility to DSS-induced colitis, and reshaped gut microbiota composition. These findings define a cooperative mechanism by which MZB1 and the J chain orchestrate IgA biogenesis, with MZB1 regulating quantity and the J chain determining quality, and reveal how variation in IgA form and abundance contributes to mucosal immune protection. - Source: PubMed
Publication date: 2026/04/17
Cui ChaoqunFeng XiaoqianMin QingLu ChunhuiWen ZichaoMeng XinZhang RunyunDong LuluWang Ji-Yang - The filamentous fungus C1 has been developed into a highly productive protein production system for heterologous proteins like antibodies and vaccine candidates. While it is capable of secreting over 120 g/l of its native enzymes, monoclonal antibodies (mAbs) have been produced at titers exceeding 20 g/l, and strains engineered to produce human-type N-glycan structures have been developed. However, significant variability in mAb productivity and reduced production levels in glycoengineered strains limit the use of C1 as a widespread production host in the pharmaceutical industry. To address these issues, transcriptome analysis was conducted on strains producing five different mAbs with varying production efficiencies, as well as on mAb-producing strains with native and glycoengineered N-glycans. Genes related to protein folding and secretion, which are regulated in response to mAb production and glycoengineering, were over-expressed in a glycoengineered mAb producing C1 strain. In addition, genes identified based on previously described functions in the secretory pathway, including counterparts of human origin, were included in the study. - Source: PubMed
Publication date: 2026/04/02
Mäkinen MariAalto AnttiPakula TiinaWiebe Marilyn GHuuskonen AnneVitikainen MarikaValkonen MariHavukainen SamiEnglund EllinorKorja VeeraEmalfarb MarkValbuena Crespo NoeliaTchelet RonenSaloheimo Markku - The emergence of immune checkpoint inhibitors (ICIs) has transformed the treatment landscape of metastatic melanoma. However, despite its success, reliable biomarkers for predicting primary resistance are not available in clinical practice. This study seeks to identify predictors of primary resistance based on novel gene expression signatures. The transcriptomic profile of the tumor microenvironment was analyzed using tissue samples from 46 metastatic cutaneous melanoma patients collected prior to the initiation of ICIs therapy. A primary resistance predictive model was trained with the Discovery FFPE RNA-seq subcohort and validated using an independent external cohort of 54 samples. Additionally, liquid biopsy samples from peripheral blood mononuclear cells were analyzed in 8 patients using single-cell RNA sequencing (scRNA-seq) and in 46 patients using flow cytometry. We identified an 82-gene transcriptomic signature composed of tumor- and immune-related genes that stratifies metastatic cutaneous melanoma patients based on primary resistance to ICIs, with key markers including and . This signature achieved an AUC of 0.814. Immune deconvolution guided by scRNA-seq revealed four immune cell subsets (Plasma cells, Pre-B cells, memory CD4⁺ T cells, and naive CD4⁺ T cells) as prognostic indicators of resistance. We propose a transcriptomic biomarker signature that accurately predicts primary resistance to ICIs in metastatic cutaneous melanoma. Through the integration of immune deconvolution with circulating immune cell profiles, we derived an ImmuneSignature linked to patient survival. By combining these approaches, we provide a framework for enhancing the prediction of immunotherapy outcomes and offer a novel strategy for identifying therapeutic targets to overcome resistance. - Source: PubMed
Publication date: 2026/03/30
Onieva Juan LuisPérez-Ruiz ElisabethVilkki VilleBerciano-Guerrero MiguelFigueroa-Ortiz LauraZalabardo ManuelMartínez-Gálvez BeatrizBarragán IsabelRueda-Domínguez Antonio - MZB1 (marginal zone B and B1 cell-specific protein 1) is an endoplasmic reticulum-resident molecular chaperone that is predominantly expressed in marginal zone B cells, B1 cells, plasma cells, and pDCs. Functioning as a co-chaperone of GRP94 and BiP, MZB1 selectively facilitates the proper folding and secretion of immunoglobulin M (IgM) and J-chain-containing immunoglobulin A (IgA) dimers, and maintains the homeostasis of highly secretory cells by upregulating the expression of partner proteins such as BiP/GRP94. This review highlights the emerging role of MZB1 across various pathological conditions. In autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), MZB1 contributes to aberrant autoantibody and cytokine secretion. MZB1 also modulates inflammatory responses in conditions such as colitis and periodontitis by regulating the B cell-skewed inflammatory microenvironment. In oncology, MZB1 is overexpressed in breast cancer (BC), lymphoma, and multiple myeloma (MM), where it is associated with enhanced tumor cell proliferation and poor prognosis. Conversely, in hepatocellular carcinoma (HCC) and gastric cancer (GC), MZB1 is transcriptionally silenced due to promoter hypermethylation. In summary, the tissue-specific expression pattern of MZB1 endows it with potential as both a diagnostic biomarker and therapeutic target, holding significant implications for the exploration of future cancer prognosis and treatment strategies. - Source: PubMed
Publication date: 2026/02/11
Zhang YuYang WeiYang XiaofangPan YuqingGuo RongChen DongTang ShicongZhang Xi