polyclonal AOX1 | alternative oxidase 1
- Known as:
- pab AOX1 | alternative oxidase 1
- Catalog number:
- as06152
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Agrisera
- Gene target:
- polyclonal AOX1 | alternative oxidase 1
Related genes to: polyclonal AOX1 | alternative oxidase 1
- Gene:
- AOX1 NIH gene
- Name:
- aldehyde oxidase 1
- Previous symbol:
- -
- Synonyms:
- AO, AOH1
- Chromosome:
- 2q33.1
- Locus Type:
- gene with protein product
- Date approved:
- 1997-04-16
- Date modifiied:
- 2016-10-05
Related products to: polyclonal AOX1 | alternative oxidase 1
Related articles to: polyclonal AOX1 | alternative oxidase 1
- Relatively little is known about receptor-mediated regulation of aldehyde oxidase (AOX). 2,3,7,8-Tetrachlorodibenzo-p-dioxin, an aryl hydrocarbon receptor (AHR) agonist, increases mouse Aox expression. However, given the species differences in ligand-dependent AHR activation and AOX regulation, it remains to be investigated whether human AHR regulates the expression of AOX1 (the human AOX isoform). Therefore, the present study was designed to determine the effect of modulating the functionality of human AHR on AOX1 gene expression in cell culture models (HepG2 and MCF-7) known to express AHR and AOX1. Treatment of HepG2 or MCF-7 cells with a noncytotoxic concentration of a classical AHR agonist (β-naphthoflavone, benzo[a]pyrene, or 3-methylcholanthrene [3-MC]), an endogenous AHR agonist (indoxyl sulfate or 6-formylindolo[3,2-b]carbazole), or a selective AHR modulator (quercetin, 3,3'-diindolylmethane, or omeprazole) increased AOX1 and AHR-regulated CYP1A1 mRNA expression. The increases in AOX1 and CYP1A1 mRNA levels were not correlated among our panel of AHR activators. The induction of AOX1 mRNA by 3-MC was time- and concentration-dependent. It was accompanied by an increase in AOX1 protein expression and AOX1-mediated enzyme activity (O-benzylguanine 8-oxidation). An AHR antagonist (3',4'-dimethoxyflavone) completely abolished the increase in AOX1 and CYP1A1 expression by an AHR agonist (3-MC). Gene knockdown by an AHR small interfering RNA substantially decreased AHR mRNA expression and attenuated AOX1 and CYP1A1 mRNA induction by 3-MC. Overall, based on the experimental approaches involving AHR activation, AHR antagonism, and AHR gene knockdown by small interfering RNA, our novel data indicate that AHR plays a role in mediating the induction of human AOX1 gene expression. SIGNIFICANCE STATEMENT: The deployment of multiple experimental approaches involving receptor activation, receptor antagonism, and gene knockdown by small interfering RNA led to the identification of the human aryl hydrocarbon receptor as a molecular mediator of aldehyde oxidase gene induction. This novel discovery expands our limited knowledge of the identity of pharmacological receptors contributing to the functionality of human aldehyde oxidase. - Source: PubMed
Publication date: 2026/07/24
Yeap Szu LingYap Cassandra Ching LinTay Shi HanZhang Linghua HarrisLau Aik Jiang - Biomass separation represents a critical bottleneck in Komagataella phaffii-based biopharmaceutical processes, as typically high cell densities of 40 - 50 % create significant operational, technical and economic challenges for harvest operations. Yeast cell aggregation (flocculation) provides a solution to accelerate cell sedimentation by increasing particle size, thus allowing to improve biomass-supernatant separation efficiency during both natural gravity settling and (continuous) centrifugation operations. This study demonstrates successful engineering of K. phaffii strains with an inducible flocculation phenotype using CRISPR/Cas9-based genome editing to integrate the Saccharomyces cerevisiae FLO1 (ScFLO1) gene under control of various regulatory elements, including methanol-inducible and derepressible promoters. Flocculation strength could be enhanced by implementing transcriptional positive feedback circuits based on the methanol-inducible AOX1 promoter. To address methanol-free production requirements, we developed alternative systems to retrofit P-based ScFLO1 expression and exploited the derepressible PDF promoter, offering broader compatibility with biopharmaceutical manufacturing facilities. Flocculating cells cultivated in a bioreactor demonstrated significantly improved sedimentation behavior, with considerably lower supernatant turbidity after short low-speed centrifugation or gravity sedimentation compared to non-flocculating controls. Crucially, cell flocculation had no negative impact on product amount and quality when expressing a multivalent NANOBODY® VHH molecule with pharmaceutical relevance. Thus, this work establishes the first genetically engineered flocculation system in K. phaffii compatible with recombinant protein production, providing the basis for an innovative approach to streamline harvest operations in biopharmaceutical processes. - Source: PubMed
Publication date: 2026/08/12
Ivanova ElenaRamp PaulZimmer NatalieMund MarkusAntonov ElenaSchiklenk ChristophDegreif Daniel - Human lactoferrin (hLF) is a multifunctional glycoprotein of the transferrin family derived from milk and mucosal secretions, which exhibits antibacterial, anti-tumor, and immunomodulatory functions, and is an important component of infant formula. Conventional methods for lactoferrin expression are often inefficient, primarily due to inadequate protein synthesis capabilities and poor stability within microbial hosts. Herein, a Komagataella phaffii yeast strain capable of high-level secretory expression of hLF was constructed by reprogramming the endoplasmic reticulum (ER) and vacuole using CRISPR/Cas9 technology. A dual-expression cassette containing the AOX1 promoter, an α-secretion signal peptide, the hLF gene, and a terminator was integrated into three different sites of the K. phaffii genome. The stepwise strategy combining expansion of the ER membrane involved in protein synthesis with knockout of vacuolar proteases further enhanced hLF production. Subsequently, 0.1 g/L FeCl₃ was added to the medium to reduce the toxicity of hLF and improve its stability. After high-density cultivation of K. phaffii through optimization of cultivation conditions in shake flasks and a 5 L bioreactor, the secretory intact hLF titer reached 2214 mg/L, representing a 76.3-fold increase achieved through these engineering strategies. In addition, antibacterial experiments demonstrated that this secretory hLF had a significant inhibitory effect on Escherichia coli, Staphylococcus aureus, and yeast. Overall, the developed K. phaffii protein expression platform enabled efficient production of lactoferrin, demonstrating its potential for expressing other lactoproteins. - Source: PubMed
Publication date: 2026/07/31
Liu KunFan XinlanTong ZhenZhang JianguoZhao MingChen YuWu ChuanchaoWang TianwenWei ShenghuaLiu YanXue ZhenglianZheng Yu - The yeast (formerly ) is a robust host for recombinant protein production, capable of growing at high cell densities, performing post-translational modifications, and efficiently secreting heterologous proteins. However, gene expression still relies mainly on the methanol-inducible promoter (P) and the constitutive promoter (P), highlighting the need for alternative promoters with distinct regulatory properties. This study aimed to identify and characterize promoters from the most highly transcribed endogenous genes across diverse culture conditions as strong promoter candidates to expand the genetic toolbox for this yeast. Seven promoters were identified through RNA-seq analysis from genes showing transcript levels comparable to or higher than those of genes regulated by P or P under specific culture conditions. These promoters were further transcriptionally characterized by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) under different carbon sources, growth phases, and temperatures through the measurement of transcript levels of their corresponding genes, revealing distinct transcriptional profiles. Two major clusters were identified: one associated with genes upregulated during the stationary phase and the other with genes upregulated during the exponential phase. These transcriptional profiles provided a basis for the rational design of fed-batch strategies tailored to each promoter when driving heterologous gene expression. The and promoters showed particular potential at low specific growth rates, favoring high recombinant protein titers with reduced biomass accumulation. analyses suggested that encodes a mitochondrial membrane-associated protein with a possible functional relationship to Hsp12. Overall, this work provides well-characterized strong promoter candidates, supporting future biotechnological applications. - Source: PubMed
Publication date: 2026/07/21
Fernández-Cano Karla BViader-Salvadó José MGuerrero-Olazarán Martha - TCDD is a long-lasting environmental pollutant. It is also grouped as a substance known to cause cancer in humans (Group 1 carcinogen). However, we do not yet fully understand the exact molecular mechanisms through which TCDD promotes the development of renal cell carcinoma (RCC). Therefore, this study aims to investigate and explain the molecular pathways that connect TCDD exposure to RCC development. - Source: PubMed
Publication date: 2026/07/17
Zheng LuopingYang JiangbinXiong BufangSu YuzheWen YaoanZheng SongZhan Shuyuan