Human Actinin Alpha 4,ACTN4 ELISA Kit
- Known as:
- Human Actinin Alpha 4,ACTN4 Enzyme-linked immunosorbent assay test Kit
- Catalog number:
- dl-actn4-hu
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Wuxi donglin
- Gene target:
- Human Actinin Alpha 4 ACTN4 ELISA Kit
Related genes to: Human Actinin Alpha 4,ACTN4 ELISA Kit
- Gene:
- ACTN4 NIH gene
- Name:
- actinin alpha 4
- Previous symbol:
- FSGS1
- Synonyms:
- -
- Chromosome:
- 19q13.2
- Locus Type:
- gene with protein product
- Date approved:
- 1992-03-26
- Date modifiied:
- 2016-10-05
- Gene:
- PMPCA NIH gene
- Name:
- peptidase, mitochondrial processing alpha subunit
- Previous symbol:
- INPP5E
- Synonyms:
- KIAA0123, Alpha-MPP
- Chromosome:
- 9q34.3
- Locus Type:
- gene with protein product
- Date approved:
- 2002-05-23
- Date modifiied:
- 2016-03-15
Related products to: Human Actinin Alpha 4,ACTN4 ELISA Kit
Related articles to: Human Actinin Alpha 4,ACTN4 ELISA Kit
- Extracellular vesicles (EVs) are secreted by any neural cells in the central nervous system for molecular clearance, cellular communications, and disease spread in multiple neurodegenerative diseases, including Alzheimer's disease (AD), although their exact molecular mechanism is poorly understood. We hypothesize that high-resolution proteomic profiling of EVs separated from animal models of AD would determine the composition of EV contents and their cellular origin. Here, we examined recently developed transgenic mice (CAST.), which express familial AD-linked mutations of amyloid precursor protein () and presenilin-1 () in the CAST/EiJ mouse strain and develop hippocampal neurodegeneration. Quantitative proteomics analysis of EVs separated from CAST. and age-matched control mice by tandem mass tag-mass spectrometry identified a total of 3444 unique proteins, which are enriched in neuron-, astrocyte-, oligodendrocyte-, and microglia-specific molecules. CAST.-derived EVs show significant enrichment of Psen1, APP, and Itgax and reduction of Wdr61, Pmpca, Aldh1a2, Calu, Anp32b, Actn4, and Ndufv2 compared to WT-derived EVs, suggesting the involvement of Aβ-processing complex and disease-associated/neurodegenerative microglia (DAM/MGnD) in EV secretion. In addition, Itgax and Apoe, DAM/MGnD markers, in EVs show a positive correlation with and mRNA expression from brain tissue in CAST. mice. These datasets indicate the significant contribution of Aβ plaque and neurodegeneration-induced DAM/MGnD microglia for EV secretion in CAST. mice and shed light on understanding AD pathogenesis. - Source: PubMed
Publication date: 2021/02/03
Muraoka SatoshiJedrychowski Mark PIwahara NaotoshiAbdullah MohammadOnos Kristen DKeezer Kelly JHu JianqiaoIkezu SeikoHowell Gareth RGygi Steven PIkezu Tsuneya