AGC1 Pre-design Chimera RNAi
- Known as:
- AGC1 Pre-design Chimera RNAi
- Catalog number:
- H00000176-R04
- Product Quantity:
- 10 nmol
- Category:
- -
- Supplier:
- Abno
- Gene target:
- AGC1 Pre-design Chimera RNAi
Related genes to: AGC1 Pre-design Chimera RNAi
- Gene:
- ACAN NIH gene
- Name:
- aggrecan
- Previous symbol:
- MSK16, CSPG1, AGC1
- Synonyms:
- CSPGCP
- Chromosome:
- 15q26.1
- Locus Type:
- gene with protein product
- Date approved:
- 1992-06-17
- Date modifiied:
- 2013-05-07
Related products to: AGC1 Pre-design Chimera RNAi
Related articles to: AGC1 Pre-design Chimera RNAi
- Bioengineered scaffolds hold promise for articular cartilage repair but are often limited by poor defect conformity, insufficient availability of endogenous reparative cells, and inadequate chondrogenic stimulation. Here, we developed a liquid-responsive shape-memory, core-shell nanofiber-reinforced, directionally porous scaffold (QCG-2%F/KGN) for endogenous cartilage regeneration. KGN-loaded SF/PCL-PVA core-shell nanofibers were fabricated by coaxial electrospinning, fragmented, incorporated into a quaternized chitosan/gelatin matrix, and assembled by directional freeze-casting. The aligned microchannels provided a structurally permissive route for cell infiltration and distribution, while the nanofiber network enhanced pore-wall roughness, structural stability, water retention, and hydration-triggered shape recovery. The core-shell fibers also enabled sustained KGN release over 30-day period. In vitro, QCG-2%F/KGN maintained high cell viability and promoted BMSC migratory activity, spreading, chondrogenic differentiation, and cartilage-matrix deposition. In a rat full-thickness cartilage-defect model, the scaffold conformally filled the defect and enhanced hyaline cartilage like regeneration, accompanied by increased SOX9, COL2A1, and ACAN and decreased COL1A1 and MMP13. Transcriptomic analysis further indicated enrichment of cartilage-anabolic programs and attenuation of inflammatory signaling. These findings support a sequential regenerative strategy integrating shape-adaptive implantation, a microarchitecture favorable for endogenous cell infiltration, and sustained chondrogenic induction. - Source: PubMed
Publication date: 2026/09/27
Zeng JiyangLi WeiLi YaweiTu ZhimingMa HongDai YuliangMa ZhaolingYuan TaoWang Bing - Chondrogenic differentiation of human adipose tissue-derived mesenchymal stem cells (ADMSCs) is a promising strategy for cartilage regeneration, but its regulatory mechanisms remain incompletely understood. MicroRNAs (miRNAs) play critical roles in post-transcriptional control of lineage commitment and tissue development. This study aimed to identify miRNAs differentially expressed during ADMSCs chondrogenesis and explore their functional roles in chondrogenic gene regulation. - Source: PubMed
Publication date: 2026/09/23
Al-Shaibani Moyassar B H - To explore the feasibility of chondrogenic differentiation of rat adipose-derived stem cells (ADSCs) combined with sodium alginate (NaAlg) hydrogel scaffolds induced by cartilage-derived morphogenetic protein 1 (CDMP-1). - Source: PubMed
Publication date: 2026/09/11
Huang ChangzhiXu NanyiZhang LeiZheng KaiyuanZhang WenrongZhuo ShiqinLi JunliWang XiaoyongLu ShuqiangLin Jiuzao - Sex modulates both the clinical presentation of schizophrenia (SCZ) and treatment response, yet its impact on the neurobiology of antipsychotic (AP) action remains insufficiently understood. Clozapine (CLZ), the gold standard for treatment-resistant SCZ, shows sex-dependent effects in both clinical and preclinical settings. We recently reported that CLZ treatment is associated with changes in extracellular matrix (ECM) organization and inhibitory circuitry in a neurodevelopmental mouse model with SCZ-relevant phenotypes, but whether these effects differ by sex remained unknown. Here, we investigated sex-dependent effects of CLZ on parvalbumin (PV) interneurons, perineuronal nets (PNNs), ECM-related gene expression, and behavior in adult mice exposed to perinatal ketamine. CLZ restored global PNN density in ketamine-exposed males, whereas in females it selectively increased PV-associated PNNs. At the molecular level, CLZ was associated with shared and sex-dependent changes in the expression of ECM-related genes, including effects on Hapln2, Reelin, and Acan. Behaviorally, CLZ improved cognitive flexibility in both sexes, but rescued social memory only in males, while females showed greater vulnerability to CLZ-induced cognitive impairment in control animals. Together, these findings reveal coordinated sex-dependent changes in PV interneuron- and ECM-related phenotypes, together with distinct behavioral responses following CLZ treatment. These observations support the hypothesis that ECM-related alterations may contribute to sex-dependent variability in CLZ response and reinforce the importance of incorporating sex as a biological variable in preclinical AP research. - Source: PubMed
Publication date: 2026/09/24
Gómez-Garrido AnaMoreno-Mellado AmandaCrespo-Facorro BenedictoGarcía-Cerro Susana - : The clinical translation of biomaterial-based strategies for ear reconstruction remains limited by several challenges, including scaffold design, selection of an optimal cell source, and stability of the new cartilage tissue. Most recent studies still rely on animal-derived cells and fail to demonstrate the long-term maintenance of the chondrogenic phenotype. : In this study, we developed an off-the-shelf poly(lactide-b-ethylene glycol) (PLA-PEG) scaffold combined with clinical-grade alginate (Alg) and cellularized with human nasal chondrocytes for auricular cartilage engineering. Cellularized constructs were evaluated using histological and immunofluorescence analyses to assess extracellular matrix production and quality. In parallel, constructs retrieved after 12 weeks of in vivo implantation were mechanically characterized using a UniVert CellScale testing system to determine variations in compressive strength. Finally, the chondrogenic maturation of the new tissue was evaluated through bulk RNA transcriptomic profiling. : Histological, immunofluorescence, and transcriptomic analyses demonstrated robust cartilage matrix deposition with improved compressive properties and high expression of chondrogenic markers, such as ACAN, PRG4, and COL2A1. Furthermore, positive staining for the human-specific Ku80 antibody confirmed the presence of cells of human origin in the newly formed tissue after implantation. : Overall, this study provides evidence that the combination of a PLA-PEG + Alg scaffold with human nasal septal chondrocytes represents a promising strategy for future reconstructive applications in auricular tissue engineering. - Source: PubMed
Publication date: 2026/09/07
Muiños-López EmmaArroyo IñigoGuaresti OlatzUrtaza UzuriBarco-Martín ArantzaUllate-Agote AsierLópez-Martínez TaniaZaldua Ane MMazo Manuel MGranero-Moltó FroilánHontanilla Bernardo