Chimpanzee IL-4 ELISPOT kit, silver staining
- Known as:
- Chimpanzee Interleukin-4 ELISPOT reagent, silver staining
- Catalog number:
- ct172-t5
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- U-CyTech biosciences
- Gene target:
- Chimpanzee IL-4 ELISPOT kit silver staining
Related genes to: Chimpanzee IL-4 ELISPOT kit, silver staining
- Gene:
- IL4 NIH gene
- Name:
- interleukin 4
- Previous symbol:
- -
- Synonyms:
- BSF1, IL-4, BCGF1, BCGF-1, MGC79402
- Chromosome:
- 5q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 1988-08-10
- Date modifiied:
- 2016-10-05
- Gene:
- TLR2 NIH gene
- Name:
- toll like receptor 2
- Previous symbol:
- -
- Synonyms:
- TIL4, CD282
- Chromosome:
- 4q31.3
- Locus Type:
- gene with protein product
- Date approved:
- 1998-06-25
- Date modifiied:
- 2016-10-25
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- Giant papillae formation along with corneal damage in vernal keratoconjunctivitis (VKC) is primarily mediated by type 2 inflammation and eosinophils. Corneal keratocytes are the primary cellular source of eotaxin at the ocular surface, facilitating eosinophil migration to the cornea. We aimed to elucidate the role of the transcription factor signal transducer and activator of transcription 6 (STAT6) in eotaxin expression by corneal fibroblasts and to evaluate the inhibitory effects of rebamipide-a mucin secretagogue with reported therapeutic potential in VKC-on this pathway. Human corneal fibroblasts were cultured and stimulated with interleukin (IL)-4 or IL-13, and subsequently preincubated with either a STAT6 inhibitor (AS1517499) or rebamipide prior to cytokine stimulation. STAT6 phosphorylation was evaluated by Western blotting. Eotaxin expression was assessed using an enzyme-linked immunosorbent assay and a real-time polymerase chain reaction. IL-4 and IL-13 stimulation induced STAT6 phosphorylation and increased eotaxin production at mRNA and protein levels. Preincubation with both AS1517499 and rebamipide suppressed IL-4- or IL-13-induced STAT6 phosphorylation, significantly reducing eotaxin mRNA and protein expression. These findings demonstrate that IL-4- or IL-13-stimulated eotaxin production in human corneal fibroblasts is mediated through the STAT6 signaling pathway. Rebamipide inhibited STAT6 activation, leading to reduced eotaxin production, suggesting that its therapeutic effect in VKC is due to the suppression of STAT6-mediated eotaxin production, which may reduce eosinophil accumulation to the cornea. - Source: PubMed
Publication date: 2026/06/26
Sakaguchi HidetoFukuda KenIshida WakaMoribe Juliana HiroyoNakajima IsanaKishimoto TatsumaYamashiro Kenji - Limited therapeutic options are available for patients with advanced-stage mycosis fungoides (MF), and the 5-year survival rate is 25%. Due to its critical role in MF pathogenesis, the IL4/IL13 pathway presents a promising target for treatment. Here, we analyzed blocking of IL4Rα, the common subunit of the IL4 and IL13 receptors, within the advanced-stage MF cutaneous tumor microenvironment (TME). - Source: PubMed
Publication date: 2026/06/25
Gaydosik Alyxzandria MWang AlysonDas JishnuLapolla BrigitAkilov Oleg EGeskin Larisa JFuschiotti Patrizia - Atopic dermatitis (AD) is a chronic immune-mediated inflammatory skin disease characterized by a complex and dynamic interplay between immune dysregulation and epidermal barrier dysfunction. Emerging evidence supports an integrated pathogenic model in which immune activation and barrier impairment form a bidirectional and self-reinforcing axis rather than representing separate processes. This review synthesizes current knowledge on the role of IL-4/IL-13-dependent signaling in regulating keratinocyte lipid metabolism and its impact on epidermal barrier integrity. IL-4/IL-13 signaling via the JAK-STAT pathway, particularly STAT6, contributes to keratinocyte dysfunction, resulting in impaired differentiation and coordinated alterations in lipid metabolism, including fatty acid elongation and ceramide synthesis. These cytokine-driven processes disrupt the organization of the stratum corneum lipid matrix, resulting in increased transepidermal water loss, enhanced skin permeability, and susceptibility to microbial colonization, thereby promoting chronic inflammation. Collectively, these findings support the concept that IL-4/IL-13-mediated dysregulation of keratinocyte lipid metabolism may represent an important immunometabolic mechanism linking type 2 inflammation with secondary barrier dysfunction in atopic dermatitis, thereby contributing to disease persistence. Targeting both immune pathways and epidermal lipid homeostasis may represent an effective strategy to restore barrier function and improve clinical outcomes. - Source: PubMed
Publication date: 2026/06/22
Andrzejczak KlaraSternak AgataWitkowski WiktorFlak AleksandraMaj JoannaPonikowska Małgorzata - To clarify the molecular mechanisms of interleukin-macrophage polarization axis in reshaping renal inflammatory microenvironment and driving chronic kidney disease (CKD) progression, and explore targeted therapeutic potential. - Source: PubMed
Publication date: 2026/06/15
Jiang ZheZhang XiaoDongJin LiJunHan MeiNiZhang YuQingJiang YuShanZhang JingluJin Lixia - Signal transducer and activator of transcription 6 (STAT6) signaling is activated by interleukin 4 (IL-4) and IL-13 and drives alternative macrophage polarization, which is pivotal in wound healing, immunosuppression, and tumor progression. STAT6 functions by forming a phosphorylated homodimer and binding to STAT6-responsive promoter elements to regulate anti-inflammatory genes. Measuring STAT6 activity can serve as a proxy for assessing macrophage polarization. We developed a STAT6-responsive-element (RE) THP-1 reporter to assess STAT6 activation in response to inflammatory stimuli. We quantitatively measured macrophage polarization by using bioluminescence temporal spectrometry (BTS). Human THP-1 monocytes were transduced with lentivirus to express STAT6-RE-firefly luciferase (FLuc)-green fluorescent protein (GFP). The STAT6-RE accurately reported endogenous STAT6 activity, as confirmed by ELISA, western blotting, bioluminescence, and imaging techniques. We developed a systems model to connect emergent bioluminescence to the kinetics of relevant STAT6 cellular signaling events. These results indicate a probe for assessing macrophage alternative polarization, enhancing our understanding of the relationship between molecular mechanisms and macrophage polarization. - Source: PubMed
Publication date: 2026/06/19
Zheng ShiyuanChiang Yi-HsuanAldarondo DasiaLocke AlexandraGunaje JagadambikaDavis JenniferFox Zachary RAkpa Belinda SWayne Elizabeth