Monkey IL-5 ELISPOT kit, enzymatic staining
- Known as:
- Monkey Interleukin-5 ELISPOT reagent, enzymatic staining
- Catalog number:
- ct129-pr5
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- U-CyTech biosciences
- Gene target:
- Monkey IL-5 ELISPOT kit enzymatic staining
Related genes to: Monkey IL-5 ELISPOT kit, enzymatic staining
- Gene:
- CSF2RB NIH gene
- Name:
- colony stimulating factor 2 receptor beta common subunit
- Previous symbol:
- IL3RB
- Synonyms:
- IL5RB, CD131, betaGMR
- Chromosome:
- 22q12.3
- Locus Type:
- gene with protein product
- Date approved:
- 1991-08-07
- Date modifiied:
- 2017-07-12
- Gene:
- IL5 NIH gene
- Name:
- interleukin 5
- Previous symbol:
- -
- Synonyms:
- IL-5, EDF, TRF
- Chromosome:
- 5q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2015-07-06
- Gene:
- IL5RA NIH gene
- Name:
- interleukin 5 receptor subunit alpha
- Previous symbol:
- IL5R
- Synonyms:
- CDw125, CD125
- Chromosome:
- 3p26.2
- Locus Type:
- gene with protein product
- Date approved:
- 1992-06-18
- Date modifiied:
- 2016-10-11
- Gene:
- LRR1 NIH gene
- Name:
- leucine rich repeat protein 1
- Previous symbol:
- PPIL5
- Synonyms:
- MGC20689, LRR-1
- Chromosome:
- 14q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 2002-11-20
- Date modifiied:
- 2014-11-18
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- Thymic epithelial tumors (TETs) are associated with Good Syndrome (GS), a secondary immunodeficiency characterized by hypogammaglobulinemia, B-cell lymphopenia, and recurrent infections. This study investigated the immunological profile of TET patients to identify immune alterations associated with GS, independently of autoimmune diseases (AD) and disease stage. - Source: PubMed
Publication date: 2026/07/04
Napolitano FabianaPietroluongo EricaDi Tolla Michele FrancescoOttaviano MargaretD'Esposito VittoriaDe Placido PietroServetto AlbertoPortella GiuseppeBianco RobertoPalmieri GiovannellaFormisano PietroMalfitano Anna Maria - Houtt. (Lamiaceae) has traditionally been used to treat respiratory disorders, including cough and dyspnea. The aim of this study was to investigate the therapeutic potential of a standardized hydroalcoholic extract from the aerial parts of in an model of allergic asthma. - Source: PubMed
Publication date: 2026/06/18
Guimarães Camila CarlaBorges Marcos De CarvalhoFabro Alexandre TodorovicCardoso Pedro HenriqueAcunha TanizeFaccioli Lúcia HelenaPereira Sarazete Izidia VazContini Silvia Helena TalebFrança Suzelei De CastroCarmona FabioPereira Ana Maria Soares - Based on the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3) signaling pathway, this study investigated the effects of Zhuyu Pills on the immune balance of helper T cell(Th) 1/Th2 and Th17/regulatory T cell(Treg) populations in a mouse model of acne. The acne model was established by applying oleic acid to the right auricle combined with subcutaneous injection of Propionibacterium acnes, resulting in auricle lesions of 1 cm~2 over a 14-day modeling period. Fifty successfully modeled ICR mice were randomly assigned to five groups: model, low-, medium-, and high-dose Zhuyu Pills, and isotretinoin(n=10 per group). An additional 10 age-matched healthy ICR mice were included as a control group without modeling. The control and model groups received equal volumes of sterile distilled water via gavage, while the low-, medium-, and high-dose Zhuyu Pills groups received Zhuyu Pills at 130.54, 261.08, and 522.16 mg·kg~(-1), respectively. The isotretinoin group received isotretinoin at 4.55 mg·kg~(-1), once daily. All treatments were administered for four weeks. After administration, auricle tissue pathology was evaluated by hematoxylin and eosin(HE) staining. Serum levels of interleukin(IL)-17, interferon-γ(IFN-γ), IL-10, transforming growth factor-β(TGF-β), IL-12, IL-22, IL-4, and IL-5 were measured by ELISA. Protein expression of p-JAK2/JAK2 and p-STAT3/STAT3 in auricle tissue was assessed by Western blot. Th1, Th2, Th17, and Treg cell populations in the spleen were analyzed by flow cytometry. Immunohistochemistry was used to detect T-box expressed in T cell(T-bet) and GATA binding protein 3(GATA3) expression, and immunofluorescence was used to detect retineic-acid-receptor-related orphan nuclear receptor gamma(RORγt) and forkhead box P3(Foxp3) expression in auricle tissue. Western blot and real-time PCR were used to measure protein and mRNA levels of T-bet, GATA3, RORγt, and Foxp3. Compared with the control group, the model group exhibited significantly thickened auricle epidermis and extensive inflammatory cell infiltration(P<0.01); increased serum IL-17, IFN-γ, IL-12, and IL-22(P<0.01); decreased TGF-β, IL-10, IL-4, and IL-5(P<0.01); elevated p-JAK2/JAK2 and p-STAT3/STAT3 levels in auricle tissue(P<0.01); increased Th1 and Th17(P<0.01) and decreased Th2 and Treg cell populations in the spleen(P<0.01); and upregulated protein and mRNA levels of T-bet and RORγt(P<0.01) but downregulated GATA3 and Foxp3 protein and mRNA levels in auricle tissue(P<0.01). Compared with the model group, all Zhuyu Pills groups and the isotretinoin group showed reduced epidermal thickness and inflammatory infiltration(P<0.01); decreased serum IL-17, IFN-γ, IL-12, and IL-22(P<0.05 or P<0.01) and increased TGF-β, IL-10, IL-4, and IL-5(P<0.05 or P<0.01); lower p-JAK2/JAK2 and p-STAT3/STAT3 levels in auricle tissue(P<0.01); decreased Th1 and Th17(P<0.01) and increased Th2 and Treg cell populations in the spleen(P<0.01); and decreased protein and mRNA levels of T-bet and RORγt(P<0.01) but increased GATA3 and Foxp3 protein and mRNA levels in auricle tissue(P<0.05 or P<0.01). In conclusion, Zhuyu Pills may regulate inflammatory cytokines through the JAK2/STAT3 signaling pathway, restore Th1/Th2 and Th17/Treg polarization balance, maintain immune cell homeostasis, and thereby exert therapeutic effects on inflammatory skin lesions in acne. - Source: PubMed
Song WeiTeng Zheng-WenZhang Zhong-YiDang Si-JieShen TaoXiao MinZhang Yong - This study aims to investigate the ameliorative effects and underlying mechanisms of Jingfang Granules(JF) combined with dexamethasone(DEX) in asthmatic mice. Balb/c mice were randomly assigned to seven groups according to body weight: normal, model, low-dose JF(1 g·kg~(-1)·d~(-1)), medium-dose JF(2 g·kg~(-1)·d~(-1)), high-dose JF(4 g·kg~(-1)·d~(-1)), DEX(1 mg·kg~(-1)·d~(-1)), and a combination of JF(4 g·kg~(-1)·d~(-1)) and DEX(1 mg·kg~(-1)·d~(-1)). The asthma model was established by intraperitoneal injection of ovalbumin(OVA) and aluminum hydroxide. Serum immunoglobulin E(IgE) and lung interleukin-4(IL-4), interleukin-5(IL-5), and interleukin-13(IL-13) levels were determined by enzyme-linked immunosorbent assay(ELISA). White blood cells(WBC), eosinophils(EOS), and lymphocytes(LYM) in bronchoalveolar lavage fluid(BALF) were counted, and T helper cell 1(Th1)/T helper cell 2(Th2) ratios were analyzed by flow cytometry to assess the anti-inflammatory and immunomodulatory effects of JF combined with DEX in asthmatic mice. Non-targeted and targeted metabolomics analyses were conducted to explore therapeutic mechanisms. The activities of superoxide dismutase(SOD), catalase(CAT), and glutathione(GSH), as well as the level of malondialdehyde(MDA), were measured by ELISA, and mitochondrial morphology was observed by transmission electron microscopy. Western blot was used to detect the expression of ferroptosis-related proteins glutathione peroxidase 4(GPX4), solute carrier family 3 member 2(SLC3A2), solute carrier family 7 member 11(SLC7A11), and acyl-CoA synthetase long-chain family member 4(ACSL4), thereby validating the findings of the metabolomics study. The results showed that compared with the model group, JF combined with DEX significantly decreased serum IgE and lung IL-4, IL-5, and IL-13 levels, reduced WBC, EOS, and LYM counts in BALF, and restored Th1/Th2 balance. Additionally, the combination therapy increased SOD, CAT, and GSH levels, lowered the MDA level, improved mitochondrial ultrastructure, and normalized GPX4, SLC3A2, SLC7A11, and ACSL4 expression. It may also inhibit ferroptosis via the regulation of amino acid metabolism. In summary, JF combined with DEX exerts synergistic anti-inflammatory, immunomodulatory, and antioxidant effects. It inhibits ferroptosis in asthmatic mice probably by regulating amino acid metabolism pathways, thereby improving the pathological state of asthma. This study provides experimental evidence supporting the use of TCM compounds combined with glucocorticoids for asthma treatment and offers novel insights into their synergistic mechanisms of intervention. - Source: PubMed
Jiao XuYang Wen-LongZhang Meng-diPan Cheng-LongLi Hong-HuaDai Guo-XinGuo NaLu Xiao-YanYao Jing-Chun - Drug reaction with eosinophilia and systemic symptoms (DRESS) is a severe T cell-mediated drug hypersensitivity reaction associated with an increased risk of multiple drug hypersensitivity (MDH). Identifying culprit drugs is challenging, particularly in poly-medicated patients, and the immunologic mechanisms underlying MDH are poorly understood. - Source: PubMed
Publication date: 2026/07/02
Thoo LesterGschwend AnnaLang ClaudiaMeier-Schiesser BarbaraPichler Werner JHausmann OliverSimon DagmarRadonjic-Hoesli SusanneYerly DanielJoerg Lukas