Human Polyclonal HSPA1A Ab
- Known as:
- Human Polyclonal HSPA1A Antibody
- Catalog number:
- a0284
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- ABclonal
- Gene target:
- Human Polyclonal HSPA1A
Related genes to: Human Polyclonal HSPA1A Ab
- Gene:
- HSPA1A NIH gene
- Name:
- heat shock protein family A (Hsp70) member 1A
- Previous symbol:
- HSPA1
- Synonyms:
- HSP70-1
- Chromosome:
- 6p21.33
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2016-10-05
Related products to: Human Polyclonal HSPA1A Ab
Related articles to: Human Polyclonal HSPA1A Ab
- In the malaria parasite , the essential chaperone Hsp70-1 regulates proteostasis through protein folding, but its domain-specific functions remain poorly defined. The protein contains an N-terminal nucleotide-binding domain (NBD) and a C-terminal substrate-binding domain (SBD), where selective inhibitors are needed to probe domain-specific functions and advance Hsp70-1 as an antimalarial drug target. Here, we identified small molecules that bind Hsp70-1 using a high-throughput thermal shift screen, an unbiased approach that enables the discovery of ligands targeting any Hsp70-1 domain. Molecules were prioritized by their affinity to Hsp70-1 and anti- activity. These efforts led to the characterization of AMK3 and AMK4, which bind to Hsp70-1 with low-micromolar affinity and exhibit selectivity (>25-fold) for Hsp70-1 over the human homolog HSPA1A based on microscale thermophoresis. The Hsp70-1 binding regions of AMK3 and AMK4 were isolated using a combination of proteomic and biochemical methods, which demonstrated that AMK3 binds to the NBD and competes with ATP binding. In contrast, AMK4 binds to the SBD and leads to disruption in peptide binding. A molecular dynamic-facilitated structure-activity relationship (SAR) screen was performed on AMK3, yielding a compound with retained anti- activity but reduced host cytotoxicity. This study identifies species-selective N-terminal and C-terminal inhibitors of Hsp70-1, and suggests druggable binding pockets on the C-terminal domain that may be exploited for the disruption of essential protein-protein interactions. These domain-specific inhibitors are useful starting points for the development of probes to advance our knowledge of Hsp70-1 functions during infection and as therapeutics to treat malaria. - Source: PubMed
Publication date: 2026/07/02
Keeler Aaron MIbrasheva GainiBoger Elizabeth GChen YueqiPetruzziello Porter ESchroeder Erin AMansfield Christopher RSylvester KaylaFleming Cameron DHughes Philip FHaystead Timothy A JFitzgerald Michael CDerbyshire Emily R - Tropical heat and humidity induce oxidative and thermal stress that threaten male fertility. However, the seminal plasma mechanisms that sustain sperm function under these conditions in goats remain poorly understood. - Source: PubMed
Publication date: 2026/01/31
Damayanti ErniDiansyah Athhar ManabiAzis Ismah UlfiyahIrawan FahrulMaulana TulusMuhajir MasturiDagong Muhammad Ihsan AndiKartika Nur Amalia - The blurry mechanism of Wuling Powder (WLP) in treating renal ischemia-reperfusion injury (R-IRI) has greatly hindered its clinical promotion and application as well as the development of modern preparations. This study applied computational systems pharmacology combined with experimental validation to reveal the mechanism of Wuling Powder (WLP) in ameliorating renal ischemia reperfusion injury (R-IRI) by regulating macrophage polarization (MP). - Source: PubMed
Publication date: 2026/06/04
Wang Qian-QianGong Yu-FanWang Zi-FengPu XingLi XiangBai LinZhang JieXie JuanLi JingJiang WeiLiu Li-MinZuo Jia-WeiZhao Ying-YongZheng Dong-HuiLi Hai-Lun - Fetal growth restriction (FGR) is frequently defined as the failure of the fetus to reach its genetically predetermined growth potential. Heat shock proteins (HSPs) are extreme-temperature-resistant molecules that help proteostasis. The aim of this prospective case-control immunohistochemistry study is to evaluate the expression of HSP90 and HSP70 in the placentas of pregnancies complicated with FGR and compare their levels with the control placentas of normal-growth pregnancies. A prospective case-control study was conducted including people undergoing singleton pregnancies who gave birth in a tertiary university hospital in Central Greece. Participants were divided into two equal groups: an FGR pregnancy group and a control group with normal growth. Immunohistochemistry of placental samples was assessed using anti-HSP90 alpha/beta antibody (clone F-8, Santa Cruz Biotechnology, Dallas, TX, USA) and anti-HSC70/HSP70 antibody (clone W27, sc-24, Santa Cruz Biotechnology, Dallas, TX, USA). A scoring system was created to quantify the expression of HSP90 and HSP70 in each sample, and the grade of staining was measured at four points. A total of 80 pregnant people were prospectively enrolled in our study, with 40 in each group. Both constitutive (HSP90β and HSC70/HSPA8) and stress-inducible (HSP90α and HSP70/HSPA1A/B) isoforms were analyzed. When comparing the total score of HSP expression, a statistically significant difference was observed for both HSP90 and HSP70. For HSP90 expression, only the Hofbauer cell's stain was identified as a statistically significant independent factor, meaning that its positive expression was observed in Hofbauer cells. For HSP70 expression, only the staining of syncytiotrophoblasts was identified as an independent factor. FGR is a common pregnancy complication and a leading cause of stillbirth, neonatal mortality, and short- and long-term neonatal morbidity worldwide. Based on our findings, the lower expression levels of both HSP90 and HSP70 are associated with FGR, revealing a possible association with stress response in FGR pathophysiology. However, more robust data from larger-scale prospective studies are needed to elucidate the possible role of HSPs as potential FGR biomarkers. - Source: PubMed
Publication date: 2026/05/27
Samara Athina AJanho Michel BZacharouli KonstantinaFloros TheodorosIoannou MariaGaras AntoniosKarachrysafi SofiaPapamitsou TheodoraMessini Christina IDaponte AlexandrosSotiriou Sotirios - Colorectal cancer (CRC) is the third most prevalent form of cancer worldwide, with colorectal cancer liver metastases (CRLM) representing a principal cause of CRC-related mortality. However, a lack of molecular subgroups based on the differentiation states of diverse cell types in CRLM poses a significant barrier to progress in precision therapy. - Source: PubMed
Publication date: 2026/06/08
Yin MinBo XiaochenLi YaoyaoYu SiminLin ZhijieWang MeiWu JianZhou MingKong LingminZhu YefeiXiao WeimingDing Yanbing