EnzyFluo AMPK Phosphorylation Assay Kit
- Known as:
- EnzyFluo AMPK Phosphorylation Assay Kit
- Catalog number:
- eampk-100
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Bioassays systems
- Gene target:
- EnzyFluo AMPK Phosphorylation Assay Kit
Related genes to: EnzyFluo AMPK Phosphorylation Assay Kit
- Gene:
- PRKAA1 NIH gene
- Name:
- protein kinase AMP-activated catalytic subunit alpha 1
- Previous symbol:
- -
- Synonyms:
- AMPKa1
- Chromosome:
- 5p13.1
- Locus Type:
- gene with protein product
- Date approved:
- 1997-05-09
- Date modifiied:
- 2016-01-27
- Gene:
- PRKAA2 NIH gene
- Name:
- protein kinase AMP-activated catalytic subunit alpha 2
- Previous symbol:
- PRKAA
- Synonyms:
- AMPK, AMPKa2
- Chromosome:
- 1p32.2
- Locus Type:
- gene with protein product
- Date approved:
- 1995-12-12
- Date modifiied:
- 2016-10-05
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- Tucum-do-Cerrado (Bactris setosa Mart.) is a polyphenol-rich Brazilian fruit known to improve glucose metabolism. This study investigated its effects on brown (BAT), inguinal (iWAT), and epididymal (eWAT) adipose tissues in a diet-induced obesity model. Rats received control (CT/TUC-), high-fat (HF/TUC-), control with Tucum-do-Cerrado (CT/TUC+), or high-fat with Tucum-do-Cerrado (HF/TUC+) diets. Tucum-do-Cerrado promoted healthier adipose expansion by stimulating adipogenic/thermogenic-related genes while downregulating lipogenesis. In BAT, HF/TUC+ attenuated adipocyte hypertrophy and decreased the number of unilocular adipocytes. Tucum-do-Cerrado downregulated Fasn mRNA levels and glutathione peroxidase (GPx) activity, whereas it increased thermogenesis-related Ucp1 mRNA. In the iWAT, HF/TUC+ decreased the adipocyte area and oxidative markers, while increasing the number of multilocular adipocytes and UCP1 protein levels. Tucum-do-Cerrado decreased Acaca, Prkaa2, Il10 mRNA, and GPx activity; conversely, it stimulated the thermogenesis-related genes Ppargc1a and Prdm16. Regarding eWAT, its consumption increased the number of multilocular adipocytes, Prkaa1, Ppargc1a, Ucp1, Prdm16, and Vegfa mRNA levels, carbonyl levels, and GPx activity, while decreasing Acaca and Fasn mRNA levels. Therefore, Tucum-do-Cerrado prevented adipocyte hypertrophy and increased the number of multilocular adipocytes. The beneficial effects of Tucum-do-Cerrado in an obesity model may be attributed to enhanced antioxidant capacity and inflammatory status, highlighting its potential in attenuating adipose tissue dysfunction. - Source: PubMed
Cavalcanti Marilia HermesDamazo Amílcar SabinoArruda Sandra Fernandes - Diabetic peripheral neuropathy (DPN) is a common diabetic complication with unclear pathogenesis. Current therapies are limited, and resveratrol shows potential in DPN but its therapeutic mechanisms remain unclear. - Source: PubMed
Publication date: 2026/06/11
Zhang ChengMo GuokangZhang YongxingXu HaoHu XiangjunZhang Jian - AMP-activated protein kinase (AMPK) serves as a crucial energy sensor, maintaining organismal energy homeostasis through the regulation of diverse metabolic pathways. However, the association between the AMPK signaling pathway and hypoxia adaptation in yak lung tissue has not yet been elucidated. Consequently, this study focused on the lung tissues of yaks and cattle residing at the same altitude (2600 m). Morphological analysis demonstrated that, compared to cattle, yak lung tissue possessed significantly thicker alveolar septa (P < 0.05), a greater abundance of elastic fibers (P < 0.05), and a reduced blood-air barrier thickness (P < 0.05), suggesting substantial structural adaptations in the yak lung under identical altitudinal conditions. RNA-seq analysis identified 3684 genes with significant differential expression between yaks and cattle. KEGG pathway enrichment analysis showed significant enrichment for the AMPK signaling pathway under the "Environmental Information Processing" category, and Gene Set Enrichment Analysis (GSEA) further confirmed the activation of the AMPK signaling pathway in yak lung tissue. Despite qRT-PCR indicating reduced mRNA levels of key AMPK pathway genes (PRKAA1, PRKAA2, PRKAB1, PRKAG2) in yak lung tissue, Western blot analysis demonstrated a marked upregulation in the relative abundance of phosphorylated AMPK (P-AMPK α1 + α2), implying potential activation of the AMPK signaling pathway via phosphorylation in yak lung tissue. Further analysis of downstream gene expression within the AMPK signaling pathway indicated significant downregulation of genes associated with glucose metabolism (PCK2, G6PC1), lipid metabolism (FASN, ACACA), protein metabolism (MAPKAPK5, MTOR), cell proliferation and apoptosis (RPTOR, MTOR), and autophagy (TXNIP, NLRP3) in yak lung tissue. These findings suggest that, relative to cattle, the yak lung may adapt to hypoxic conditions by minimizing energy expenditure, suppressing aberrant cell proliferation, mitigating oxidative stress, and reducing inflammatory responses. In summary, the activation of the AMPK signaling pathway in yak lung tissue may play a crucial role in hypoxic adaptation by enhancing oxygen utilization and energy supply capacity. - Source: PubMed
Publication date: 2026/04/04
Zhang XunDing WeiqinWang HuizhenLi JingyiChen JiaruiWei Qing - Ventromedial hypothalamic nucleus/dorsomedial division (VMNdm) metabolic-sensory growth hormone-releasing hormone (Ghrh) neurons operate within the brain circuitry that maintains systemic glucostasis. Sex-specific counterregulatory hormone adaptation to recurrent insulin-induced hypoglycemia (RIIH) entails undiscovered CNS mechanisms. Combinative single-cell immunocytochemistry/laser-microdissection/multiplex qPCR methods were employed here to determine if RIIH alters VMNdm Ghrh neuron neurotransmission according to sex. Precedent insulin administration resulted in attenuated hypoglycemic up-regulation of male rat Ghrh neuron glucose sensor gene expression. Females exposed to RIIH acquired negative glucose transporter-2 and positive glucokinase transcriptional reactivity to dysglycemia. VMNdm Ghrh neurons express mRNAs for 5'-AMP-activated protein kinase catalytic subunit variants. Hypoglycemia-associated amplification of those transcripts in the male was exacerbated (PRKAA1) or reversed (PRKAA2) by RIIH; meanwhile females showed transcript up-regulation during recurring but not singular hypoglycemia. RIIH caused opposite, sex-specific adaptation of VMNdm Ghrh neuron Ghrh and Ghrh receptor gene transcription. Ghrh neuron transcripts for counterregulation-enhancing glutamate and nitric oxide marker proteins showed diminution or amplification of transcriptional responses, respectively, in males, but did not habituate to RIIH in females. Hypoglycemic inhibition of glutamate decarboxylase (GAD)-1 and - 2 mRNAs, markers for counterregulatory inhibitor γ-aminobutyric acid size variants, in male Ghrh neurons was correspondingly unaffected or attenuated by RIIH. Meanwhile, negative GAD2 transcriptional reactivity in the female was exacerbated following precedent hypoglycemia. Ongoing research aims to establish, for each sex, whether and how VMNdm Ghrh neuron metabolic sensor functional acclimation to RIIH may affect counterregulatory neurotransmitter release and to determine how adjustments in integrated neurochemical discharge may affect brain glucostatic network function. - Source: PubMed
Publication date: 2026/03/22
Yadav RajeshSapkota SubashBriski Karen P - Livsooth Authentic Herbal Formula (LAH) is a novel Chinese herbal medicine that has been previously shown to prevent non-alcoholic fatty liver disease (NAFLD). However, its efficacy in treating obesity and its underlying mechanisms remain unclear. This study uniquely investigates the therapeutic effects of LAH on high-fat diet (HFD)-induced obese mice, focusing on its multi-targeted regulation of metabolic pathways. This research highlights the potential of a multi-component herbal formula in simultaneously activating the AMPK pathway, regulating lipid metabolism, and enhancing antioxidant defenses. By integrating network pharmacology predictions with proteomics analysis, , and experiments, this study provides a comprehensive understanding of LAH's mode of action. - Source: PubMed
Publication date: 2025/03/05
Chen Yu-JuNing De-ShanWang Ching-ChiungZhao Hong-WeiWang Kun-TengLee Ming-ChungChiu Wan ChunYeh Chiu-LiJacinto Dela Vega John LouieLee Chia-Jung