PYK2 (Phospho-Tyr579) Antibody
- Known as:
- PYK2 (Phospho-Tyr579) Antibody
- Catalog number:
- 11776
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Signalway
- Gene target:
- PYK2 (Phospho-Tyr579) Antibody
Related genes to: PYK2 (Phospho-Tyr579) Antibody
- Gene:
- PTK2B NIH gene
- Name:
- protein tyrosine kinase 2 beta
- Previous symbol:
- FAK2
- Synonyms:
- CAKB, PYK2, RAFTK, PTK, CADTK
- Chromosome:
- 8p21.2
- Locus Type:
- gene with protein product
- Date approved:
- 1998-06-22
- Date modifiied:
- 2017-12-06
Related products to: PYK2 (Phospho-Tyr579) Antibody
Related articles to: PYK2 (Phospho-Tyr579) Antibody
- Spermatogonial stem cells (SSCs) sustain spermatogenesis throughout life by generating differentiating spermatogonia, spermatocytes, and spermatozoa. During this process, male germ cells are interconnected by intercellular bridges (ICBs). Although ICB fragmentation has been implicated in the replenishment of the SSC pool, the molecular mechanisms underlying this process remain poorly understood. Our previous study demonstrated that JMJD3 deficiency promotes spermatogonial fragmentation and preserves spermatogenesis during ageing. To investigate the molecular basis underlying these phenotypes, we performed H3K27me3 ChIP-seq using a spermatogonia-enriched CD9-positive cell fraction together with RNA-seq of SSC-enriched spermatogonial populations from JMJD3-deficient mice. Cross-dataset comparison revealed extensive alterations in H3K27me3 landscapes accompanied by age- and JMJD3-dependent transcriptional changes. RNA-seq demonstrated downregulation of multiple signaling pathways, including NF-κB and TNF signaling, together with dysregulation of extracellular matrix (ECM)- and cell adhesion-related genes in JMJD3-deficient spermatogonia. Consistent with these transcriptional changes, JMJD3-deficient spermatogonia exhibited excessive ECM accumulation and altered expression of adhesion-associated genes in vivo. Although several mesenchymal-associated genes were upregulated, canonical cadherin switching was not observed, indicating the presence of partial epithelial-mesenchymal transition (EMT)-like transcriptional features rather than a complete EMT program. Cross-dataset comparison further identified Ptk2b as a candidate gene associated with JMJD3-dependent H3K27me3 changes. These findings suggest that JMJD3 deficiency is associated with extensive epigenomic remodeling and adhesion-related transcriptional alterations in aged spermatogonia and provide candidate molecular pathways that may contribute to the maintenance of spermatogenesis during ageing. - Source: PubMed
Sakurai YusukeNomura HozumiAoyama KazumasaShima SakurakoYoshida AyaIwamori TokukoIwama AtsushiIwamori Naoki - In the developing human placenta, three subtypes of trophoblast cells, cytotrophoblasts (CTBs), extravillous trophoblasts (EVTs), and syncytiotrophoblasts (STBs), mediate critical functions essential for a successful pregnancy. CTBs constitute the stem/progenitor compartment and differentiate into STBs and EVTs within the floating and anchoring villi, respectively. STBs establish the maternal-fetal exchange interface and secrete human chorionic gonadotropin (hCG), a hormone vital for the maintenance of early pregnancy. EVTs anchor the maternal endometrium and invade the uterine tissue to remodel maternal cells, supporting implantation and progression of pregnancy. In this study, we used human trophoblast stem cells (hTSCs) as a model system and performed quantitative, label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) to profile the proteome and phosphoproteome in TSC stem state (analogous to undifferentiated CTBs) and following their differentiation to STBs and EVTs. Through a multiomics approach, we integrated our proteomics data with global gene expression profiles to correlate cell-type specific gene and protein expression during human trophoblast development. We also identified global phosphoproteome and analyzed kinases that are specifically active in hTSC stem state, as well as in differentiated STBs and EVTs. We experimentally validated specific kinases, such as BUB1B, PAK6, PKYMT1, and TNIK, that are essential for maintaining the hTSC stem-state. Additionally, atypical protein kinase C isoforms PKCζ are essential for STB development, whereas PTK2B, SRC, TRIO, and LYN are important for EVT development. Our findings highlight key kinases uniquely required for specific stages of trophoblast development during human placentation and suggest that pharmacological inhibition of these kinases could negatively impact the placentation process during pregnancy. - Source: PubMed
Kumar RajnishDasgupta PurbasaRay SomaPaul Soumen - Fibrosis, a common end point of chronic inflammatory diseases across organs, remains a major therapeutic challenge. Macrophages are central regulators of fibrotic remodeling, with evidence suggesting their direct role through macrophage-to-myofibroblast transition. However, the upstream mechanisms governing macrophage fibrogenic reprogramming remain unclear. Using a laser-induced mouse model of subretinal fibrosis, whether adhesion-dependent mechanotransduction regulates macrophage-driven fibrosis was investigated. Bulk RNA sequencing of retinal pigment epithelium-choroid tissues revealed significant enrichment of adhesion-related genes and focal adhesion pathways, with up-regulation of integrins such as Itgb2, Itgal, and Itgax. Increased integrin expression and focal adhesion kinase (FAK) phosphorylation were detected in infiltrating F4/80 macrophages within fibrotic lesions. Bone marrow-derived macrophages under high adherence conditions expressed higher levels of fibrosis-related genes and FAK-related genes such as Ptk2 and Ptk2b. FAK inhibitor PF562271 suppressed transforming growth factor-β1-induced up-regulation of fibrosis-related genes (Col1a1, Fn1, and Acta2) and α-smooth muscle actin in macrophages. I.P. administration of PF562271 reduced expression of adhesion molecules ITGB2 and ITGAL on circulating monocytes and alleviated subretinal fibrosis. These findings identify integrin-FAK-mediated mechanotransduction as a key regulator of macrophage fibrogenic reprogramming and macrophage-to-myofibroblast transition. Adhesion-dependent signaling is highlighted as a conserved pathway linking tissue remodeling to fibrotic macrophage activation and FAK as a potential therapeutic target. - Source: PubMed
Publication date: 2026/08/29
Deng WenYi CaijiaoLiu JianCui XuexueYe ChanghuaChen ZhongpingChen MeiXu Heping - Diabetic retinopathy (DR) remains a leading cause of vision loss, with macrophages and glycosylation dysregulation implicated in DR pathogenesis. However, the potential as diagnostic biomarkers has been rarely investigated. - Source: PubMed
Publication date: 2026/08/27
Pan ZhujuanZhou ShaoboQi WenjuanWang JingLuo YanFan Feihong - Residual feed intake (RFI) is an indicator of feed efficiency that reflects variation in nutrient utilization independent of growth. This study characterized physiological traits and multi-omics profiles associated with divergent RFI in small-sized meat ducks. From an initial population of 500 1-day-old ducks, a total of 420 healthy ducks were individually housed from 21 to 42 d to record feed intake, and ducks with low RFI (LRFI) and high RFI (HRFI) were identified for further analyses. During the experiment, 30 ducks per group for growth performance, 15 ducks per group for plasma biochemical and 5 per group for multi-omics. Compared with HRFI ducks, LRFI ducks showed lower feed intake, lower feed conversion ratio (FCR), and lower plasma triglyceride concentrations, whereas body weight gain did not differ between groups. Shotgun metagenomic analysis showed that LRFI ducks were enriched in Bacteroides-related lineages and had higher predicted capacities for complex carbohydrate degradation, lipid and energy metabolism, and cofactor synthesis, whereas HRFI ducks were enriched in taxa including Subdoligranulum variabile and Clostridioides difficile. Untargeted cecal metabolomics revealed distinct lipid- and bile acid-related metabolic profiles between the 2 groups, including differences in long-chain lipid species and bile acid-associated metabolites. Hypothalamic transcriptomic analysis identified differentially expressed genes related to neuropeptide signaling, serotonin biosynthesis, intracellular signaling, and inflammatory regulation, including NMUR2, TPH1, and PTK2B. Correlation analysis integrating microbial taxa, metabolites, and hypothalamic transcripts further revealed coordinated associations among these features in small-sized meat ducks with divergent RFI. Overall, variation in feed efficiency in ducks was associated with differences in cecal microbiota, metabolite profiles, and hypothalamic gene expression, and these results highlight candidate microbial taxa, metabolites, and genes for further validation. - Source: PubMed
Publication date: 2026/06/17
Geng DandanDing YifanJiang YongWang ZhixiuChen GuohongChang GuobinBai Hao