eNOS (Phospho-Thr494) Antibody
- Known as:
- eNOS (Phospho-Thr494) Antibody
- Catalog number:
- 11711
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Signalway
- Gene target:
- eNOS (Phospho-Thr494) Antibody
Related genes to: eNOS (Phospho-Thr494) Antibody
- Gene:
- NOS3 NIH gene
- Name:
- nitric oxide synthase 3
- Previous symbol:
- -
- Synonyms:
- ECNOS, eNOS
- Chromosome:
- 7q36.1
- Locus Type:
- gene with protein product
- Date approved:
- 1993-08-23
- Date modifiied:
- 2016-10-05
Related products to: eNOS (Phospho-Thr494) Antibody
Related articles to: eNOS (Phospho-Thr494) Antibody
- Atherosclerosis (AS) is a major global health burden. Sodium nitrite, a common environmental and dietary contaminant, has been implicated in promoting AS, but its systematic molecular mechanisms remain unclear. - Source: PubMed
Publication date: 2026/05/05
Yang HaoBoYu YunFengZhang YongHuiBai YaNanShi YaRuJian WeiXiong - Preeclampsia (PE) is the primary cause of maternal and perinatal morbidity and mortality on a global scale. It is driven by a multifactorial aetiology, in which genetic factors involved in blood pressure regulation, including the endothelial nitric oxide synthase () gene, play an important role. This study aimed to investigate the association between gene polymorphisms and the development and severity of PE in an Algerian cohort. - Source: PubMed
Publication date: 2026/03/30
Atmani Sara MimiBouldjennet FaizaKhalili YasmineFeghoul NabylaDammene Debbih Amel - Most glaucoma genetic data derive from European and East Asian cohorts, leaving high-consanguinity Middle Eastern populations under-characterized. This review synthesizes 33 Saudi-specific genetic studies (2014-2024, >9000 participants) to define a population-level glaucoma genetic architecture that diverges substantially from global models and carries direct precision medicine implications. Three findings distinguish the Saudi landscape. First, functions as the dominant causal gene across both primary congenital glaucoma (PCG) and juvenile-onset open-angle glaucoma (JOAG), accounting for 76-86% of cases, with two founder alleles, p.G61E (penetrance 87.7%) and p.R469W (penetrance 93%), driving severe, early-onset phenotypes. Critically, and , the primary JOAG genes in other populations, carry no pathogenic variants in Saudi cohorts, rendering standard multi-ethnic gene panels inadequate for this population. Second, adult-onset glaucoma follows a distinct polygenic architecture where ε2 confers a near five-fold risk for primary angle-closure glaucoma (OR = 4.82), an effect absent or inconsistent in global datasets, and variants associate with primary open-angle glaucoma specifically in men, a sex-stratified signal unreported outside Saudi cohorts. The T/T genotype, common in European and Asian POAG patients, is entirely absent locally, indicating population-specific allelic distributions that alter folate-metabolism-related optic nerve susceptibility. Third, rs12997 associates across POAG, PACG, and pseudoexfoliation glaucoma (PXG), positioning BMP/TGF-β signaling as a shared mechanistic pathway spanning multiple subtypes. These findings argue for Saudi-specific genetic panels, -centered cascade testing in consanguineous families, and polygenic risk models incorporating local allele frequencies rather than globally derived weights. - Source: PubMed
Publication date: 2026/04/14
Alotaibi Abdullah FaisalMaawadh Lojain Mohammed AAlmutairi Mohammed Naji ObaidHameed SyedMalik RizwanAbu-Amero Khaled K - Hydroxyurea (HU) is a ribonucleotide reductase inhibitor widely used for the treatment of sickle cell disease and myeloproliferative disorders, yet a precise nitric oxide (NO) synthase (NOS)-dependent mechanism remains incompletely defined. The role of NOS3 in HU-mediated proliferation, cell cycle, and apoptosis was analyzed in HEL92.1.7 erythroleukemic cells and primary mouse erythroid progenitors upon genetic knockdown/knockout and pharmacological NOS2/NOS3 inhibition. NOS3 expression, phosphorylation, NO and citrulline production, and protein nitrosylation were assessed via immunoblotting and biochemical assays. Computational docking and molecular dynamics simulations were performed to examine the interaction between HU and NOS3. HU enhanced NOS3 expression and phosphorylation, leading to increased NO and citrulline production. Computational analysis predicted HU binding within the NOS3 active site, whereas functional activation was AKT1-dependent. A biotin switch assay revealed cooperative NOS2-/NOS3-mediated protein nitrosylation under HU treatment. NOS3 depletion or inhibition abrogated HU-induced S-phase accumulation and restored cell proliferation. NOS3 protein depletion increased late apoptosis in erythroleukemic cells, while in murine erythroid cells, both Nos3 deficiency and inhibition decreased early and increased late apoptosis. NOS2 and NOS3 act as complementary mediators of proliferation and apoptosis, with NOS3 playing a distinct role in HU-induced proliferation arrest in erythroid cells. These findings highlight the therapeutic potential of NOS targeting to enhance the efficacy of HU and overcome resistance in hematologic malignancies. - Source: PubMed
Publication date: 2026/03/31
Dragojević TeodoraĐikić DragoslavaMojsilović SlavkoLazarević MilošMilenković DejanAjtić Olivera MitrovićŽivković EmilijaDiklić MilošSubotički TijanaSantibanez Juan FČokić Vladan PVukotić Milica - Quality standardization of Indian medicinal plants is crucial for confirming their safety, efficiency, and validity in pharmaceutical applications. The current investigation explores ethnopharmacological perspectives of Allium sativum and Syzygium jambolanum through phytochemical screening using HPTLC and LC-MS profiling, and systems-based pharmacology investigation using network pharmacology and molecular docking studies. The results indicated that both plants are enriched in polyphenols, terpenoids, glycosides, etc., responsible for various biological activities. In vitro antioxidant activity demonstrated significant free-radical neutralization potential. HPTLC showed major and minor metabolites at different R values, and LC-MS analysis showed key bioactive substances including ferulic acid, gallic acid, naringenin, catechin, quercetin, and myricetin. The Network pharmacology study identified interactions with key proteins, including IL-1β, TLR4, IL6, NOS3, ABCA1, with prominent interaction of quercetin and kaempferol with the protein IL-1β. The DisGeNET mapping findings point toward their participation in key pathophysiological conditions like inflammation, interstitial inflammation, myofibroblast activation causing tubulointerstitial fibrosis, and microbial infection. The study concludes that A. sativum and S. jambolanum actively play a significant role in inflammation and associated dysfunctions, hence providing novel plant-based therapeutics as A. Sativum and S. jambolanum by highlighting the significance in traditional medicine as well as their potential relevance in contemporary pharmacology. - Source: PubMed
Singh ManjuGaurav Gaur Praveen Kumar