c-fos pThr232 antibody Ab
- Known as:
- c-fos pThr232 (anti-) Antibody
- Catalog number:
- 1488188
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Acris antibodies
- Gene target:
- c-fos pThr232 antibody
Related products to: c-fos pThr232 antibody Ab
Related articles to: c-fos pThr232 antibody Ab
- Postmenopausal osteoporosis (PMOP) is a metabolic bone disorder driven by excessive osteoclast (OC) activity, but current therapies are limited by adverse effects. Trans-zeatin (TZ), a naturally occurring plant cytokinin with antioxidant properties, has not been investigated in bone metabolism. Here, we show that TZ suppresses receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis and bone resorption in vitro and attenuates ovariectomy-induced trabecular bone loss in mice. Mechanistically, TZ activates nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated antioxidant responses by upregulating heme oxygenase-1 (HO-1) and catalase while suppressing NADPH oxidase 4 (NOX4) expression, thereby reducing reactive oxygen species (ROS) accumulation. This redox modulation subsequently dampens protein kinase B (AKT) signaling, downregulates the c-Fos/nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) transcriptional program, and decreases the expression of OC-specific effector genes. Notably, Nrf2 knockdown partially reverses the inhibitory effects of TZ, whereas concurrent AKT knockdown attenuates this rescue effect, suggesting that AKT is an important downstream signaling component associated with Nrf2-mediated redox regulation. Collectively, these findings identify TZ as a promising natural candidate for PMOP intervention through coordinated regulation of redox homeostasis and AKT-dependent osteoclastogenic signaling. - Source: PubMed
Publication date: 2026/09/25
Xiao YongLu QichenShi MiaomiaoLiang HaiboLuo JiangtaoWu YinuoWang LinxuanNan YuWei RongjieMiao JiansenTu YitingLiu YuhaoSheng SunrenJin Haiming - Negative energy balance increases glucocorticoids (GC) and inhibits reproduction; however, the neurocircuitry mediating these effects remains poorly understood. We hypothesized that GC signaling in leptin receptor (Lepr)-expressing neurons mediates reproductive disruption caused by metabolic stress. - Source: PubMed
Publication date: 2026/09/25
Pereira Sidney AMeireles Cinthia GMin LeMagnuson MelissaZhou YingXia HexiaFontes Audrey NKim AshleyArvizu-Sanchez LilianaSurve Aisha A WZdon Samuel TNaule LydieGarza VivianaKim Hans KyeolLofrano-Porto AdrianaCarroll Rona SKaiser Ursula B - THP-1, a widely used human monocytic leukemia cell line, has been described as one of the hard-to-transfect suspension cell lines. Here, we present a rapid and efficient method for generating multiplex gene knockout cells from THP-1 using a simple co-transfection strategy based on the CRISPR-Cas9 system. In this method, THP-1 cells were transfected with a mixture of pX330-based sgRNA plasmids and a small amount of puromycin-resistance plasmid by electroporation, followed by transient selection with high-dose puromycin. This simple method resulted in efficient simultaneous knockout of multiple genes. For genes important for survival and/or cell proliferation, gene-knockout clones may be isolated by the limiting dilution method immediately after puromycin selection.•A simple co-transfection strategy to knock out multiple genes simultaneously in the human monocytic leukemia cell line, THP-1.•Transient selection with high-dose puromycin appeared to be effective for efficient knockout.•In general, knockout efficiency is satisfactory, and polyclonal knockout cells can be used without a cloning step unless the target gene plays a critical role in cell survival or proliferation. - Source: PubMed
Publication date: 2026/09/16
Ito ChihiroKinomura YukiKawagoe KenshoFujii ShinobuFujii ToshihiroFukunaga Rikiro - Prebiotics are nondigestible food components that selectively stimulate beneficial gut microbiota and have garnered significant attention in food science and biotechnology because of their health-promoting properties. Among them, fructooligosaccharides and galactooligosaccharides are especially important for their positive effects on human health. Functional foods with enhanced nutritional and medicinal value have been made possible by the recent use of prebiotics in food fermentation and bioprocessing. This review summarizes recent developments in prebiotic production and application, with emphasis on biotechnological approaches such as microbial fermentation, enzymatic synthesis, and strain improvement techniques, based on literature retrieved from PubMed, Scopus, and Web of Science up to July 2025. Advances in fermentation technologies, including solid-state fermentation and co-culture systems, are also discussed for optimizing production processes. In addition, the review highlights the health-promoting effects of prebiotics, including modulation of gut microbiota, immunological regulation, and involvement in the gut-brain axis. Lastly, we highlighted current challenges and prospects, emphasizing their application to transform fermented foods and bioprocessing techniques. - Source: PubMed
Publication date: 2026/09/22
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Publication date: 2026/09/24
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