HCK & PIK3CB Protein Protein Interaction Antibody Pair
- Known as:
- HCK & PIK3CB Protein Protein Interaction Antibody Pair
- Catalog number:
- DI0014
- Product Quantity:
- 1 Set
- Category:
- -
- Supplier:
- Abno
- Gene target:
- HCK & PIK3CB Protein Interaction Antibody Pair
Related genes to: HCK & PIK3CB Protein Protein Interaction Antibody Pair
- Gene:
- PIK3CB NIH gene
- Name:
- phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
- Previous symbol:
- PIK3C1
- Synonyms:
- -
- Chromosome:
- 3q22.3
- Locus Type:
- gene with protein product
- Date approved:
- 1992-12-18
- Date modifiied:
- 2015-11-17
Related products to: HCK & PIK3CB Protein Protein Interaction Antibody Pair
Related articles to: HCK & PIK3CB Protein Protein Interaction Antibody Pair
- To enhance risk stratification in metastatic castrate-resistant prostate cancer (mCRPC), we developed clinical and integrated clinico-genomic prognostic nomograms combining clinical prognostic factors with copy number alteration-based risk scores (RSs) in circulating tumor DNA (ctDNA) and metastatic tissue to predict 1-, 2-, and 3-year overall survival (OS) probabilities. - Source: PubMed
Kohli ManishShankar AnushkaLloyd JenniferWang LiangHuo XingyueFadlullah Muhammad ZakiTan Aik ChoonFinkelstein Joseph - Precocious puberty in chickens is an important biological trait regulated by the hypothalamic-pituitary-gonadal (HPG) axis. The comb height of roosters is an indicator of precocious puberty in breeding. However, the underlying mechanisms of precocious puberty, particularly in roosters, remain underexplored. - Source: PubMed
Publication date: 2026/05/25
Xiang HaiLi XiangkunSun XiaoxuZhang ZhengfenWei JixiangLiu ZhijieMa ZhengChen SiyuGou JunweiYe FeiLi Hua - Brain abundant membrane attached signal protein 1 (Basp1), a member of the neuronal growth-associated protein family, was originally identified for its high expression in the central nervous system. Recent evidence suggests its involvement in peripheral diseases through modulation of myeloid cell function; however, the molecular mechanisms remain poorly defined. In this study, by re-analyzing single-cell RNA sequencing data from peripheral blood of TBI patients and validating results in a mouse TBI model via flow cytometry, we demonstrated that Basp1 was specifically upregulated in neutrophils after injury. Using tamoxifen-induced lineage tracing (Basp1⁺-TdTomato⁺), we further confirmed that Basp1-expressing neutrophils represented the majority of infiltrated neutrophils in the brain during acute TBI. Bulk RNA-seq comparing Basp1⁺ and Basp1 neutrophils revealed Basp1's central role in regulating neutrophil migration and neutrophil extracellular traps (NETs) formation. Silencing Basp1 impaired both processes in vitro, and real-time PCR analysis indicated correlated downregulation of key chemotaxis-related (Itgb2, Cybb, Mmp9, Actn1, Cxcr2, Pik3cb) and NETs-related genes (Fcgr1, H3c1, Tlr2). Protein docking and co-immunoprecipitation assays demonstrated a direct interaction between Basp1 and Vimentin in neutrophils, suggesting a mechanism by which Basp1 promotes cytoskeletal rearrangement essential for migration and NETs formation. Notably, conditional KO Basp1 in myeloid cells significantly reduced neutrophil infiltration and NETs formation after TBI. Our work identifies Basp1 as a critical molecular mediator of neutrophil-driven inflammation in TBI, providing a potential therapeutic target not only for TBI but also for other neuroinflammatory conditions. - Source: PubMed
Publication date: 2026/05/18
Sun JianbinLi TaoZhang TongYang TengMa PengjiaoZhou RuitianTan MenglanHuang YvshaHe WenhuiDai Shuang-ShuangLiu Yang-Wuyue - Strong-flavor-type Baijiu, represented by -a renowned traditional Chinese alcoholic beverage brewed from five grains (sorghum, rice, glutinous rice, wheat, and corn)-is widely consumed and appreciated for its balanced taste and potential health benefits. While the volatile flavor compounds of Baijiu have been well studied, its bioactive components and their underlying mechanisms remain insufficiently explored. In this study, widely targeted metabolomics techniques were innovatively employed, and 2128 compounds were identified from 10 samples, of which 445 were predicted to constitute potential bioactive substances. Network pharmacology analysis further identified four key compounds, namely the four potential bioactive small molecules (fisetin, luteolin, norartocarpetin, and scutellarein), along with ten core targets that were key protein targets interacting with these compounds (SRC, PIK3R1, PTGS1, AKR1B1, STAT3, CYP3A4, ESR1, PIK3CA, PIK3CB, and ALOX15). GO and KEGG enrichment analyses indicated that these targets participated in diverse biological processes, while DO analysis revealed potential associations between these targets and specific diseases. Additionally, molecular docking confirmed the binding patterns between the identified compounds and their targets. Collectively, this study provides systematic chemical information and theoretical screening results for identifying potential bioactive components in strong-flavor-type Baijiu, which may facilitate further studies of their biological functions. - Source: PubMed
Publication date: 2026/04/27
Liu JinxiaoZheng JiaWu JihongSun YingHuang MingquanSu JianZheng FupingZhao Dongrui - : Hepatitis B virus (HBV) infection continues to be a major public health concern, especially in sub-Saharan Africa, where widespread epidemics and restricted availability of long-term antiviral therapies result in higher mortality and morbidity rates. Drug repurposing represents a strategic approach to accelerate the discovery of effective therapies by leveraging agents with demonstrated antiviral and immunomodulatory activity. Product Nkabinde (PN) is a patented African polyherbal formulation initially developed for the treatment of HIV. Recent experimental studies demonstrate PN's potent anti-HIV activity and significant immunomodulatory effects in human immune cells, implicating host-directed mechanisms relevant to chronic viral infections. This study combines an integrative application of network pharmacology and molecular docking to evaluate the repurposing potential of PN as a multi-target agent in HBV. : Bioactive components of PN were screened, and compound-associated targets were intersected with HBV-associated genes (proteins) to construct a protein-protein interaction (PPI) network. Topological analysis identified 10 hub targets (STAT1, STAT3, SRC, HCK, EGFR, SYK, PIK3CA, PIK3CB, PIK3R1, and PTPN11). Gene Ontology and KEGG pathway enrichment were performed with an FDR cut-off < 0.05. Significantly enriched pathways included JAK-STAT signaling, chemokine signaling, EGFR-TKI resistance, PI3K complex signaling, and viral infection pathways, particularly those related to Kaposi sarcoma virus and HSV-1, indicating immunoregulatory and antiviral roles. Molecular docking was performed using AutoDock Vina 1.1.2 to evaluate binding affinity and interaction mode of key PN phytochemicals against the hub proteins, and results were compared to their respective co-crystallized ligands. : Molecular docking indicated that major phytochemicals from PN exhibited significant binding affinities across all 10 hub host targets, typically outperforming or closely matching their respective co-crystallized ligands. The strongest contacts were observed for β-sitosterol-PIK3CB (-14.2 kcal/mol) and oleanolic acid-SYK (-14.0 kcal/mol), which were significantly stronger than the co-crystallized ligands (-7.9 and -8.3 kcal/mol, respectively), indicating robust stabilization within catalytic and regulatory pockets. Procyanidin B2 toward HCK (-10.5 vs. -7.9 kcal/mol) and PIK3CA (-9.5 vs. -7.3 kcal/mol), quercetin toward PIK3R1 (-10.6 vs. -8.2 kcal/mol) and PTPN11 (-9.2 vs. -7.5 kcal/mol), rutin toward SRC (-10.5 vs. 7.8 kcal/mol), and diosgenin toward EGFR (-9.4 vs. 8.4 kcal/mol). Procyanidin B2 maintained robust multi-hydrogen bonding networks, demonstrating significant binding, despite STAT1 and STAT3 docking showing identical affinities to co-crystals. Conserved hydrogen bonds, π-cation interactions, and significant hydrophobic packing at ATP-binding clefts and regulatory domains supported these interaction patterns, indicating competitive suppression of host signaling nodes taken over by HBV. : Together, these results demonstrate that the components of PN possess strong multitarget binding capabilities across the PI3K/AKT, JAK-STAT, SRC-family kinase, EGFR, and SYK pathways, supporting their potential repurposing as host-directed HBV therapeutics with the ability to impede immune evasion, viral persistence, and HBV-associated oncogenic progression. - Source: PubMed
Publication date: 2026/04/16
Ugbaja Samuel ChimaNkabinde Siphathimandla AuthorityNkabinde MaguguGqaleni Nceba