Pou5f1 polyclonal antibody
- Known as:
- Pou5f1 pab (anti-)
- Catalog number:
- PAB18963
- Product Quantity:
- 100 ug
- Category:
- -
- Supplier:
- Abno
- Gene target:
- Pou5f1 polyclonal antibody
Related genes to: Pou5f1 polyclonal antibody
- Gene:
- POU5F1 NIH gene
- Name:
- POU class 5 homeobox 1
- Previous symbol:
- OTF3
- Synonyms:
- OCT3, Oct4, MGC22487
- Chromosome:
- 6p21.33
- Locus Type:
- gene with protein product
- Date approved:
- 1992-11-05
- Date modifiied:
- 2016-04-25
Related products to: Pou5f1 polyclonal antibody
Related articles to: Pou5f1 polyclonal antibody
- POU5F1 (OCT4), a core regulator of pluripotency, plays an important role in tumor stemness and immune microenvironment remodeling, yet its systematic function and mechanisms in lung adenocarcinoma (LUAD) remain incompletely elucidated. - Source: PubMed
Publication date: 2026/05/06
Yu BingbingZhang MeiliZhu HaiyongWu ZhuGao HongBai Yang - The emergence of scRNA-seq has enabled high-resolution gene expression analysis at the single-cell level, providing important opportunities for inferring gene regulatory networks (GRNs) within individual cells. This study proposes a novel method, termed Topological Data Analysis-guided Gene Network Embedding (TDAGENE), which introduces the topological data analysis (TDA) to enhance the GRN inference. It integrates the global topological feature with local graph representation and, therefore, improves its ability to model the gene expression by capturing the topological structure of GRN and facilitate the identification of gene interaction relationships. Various experiments demonstrate that TDAGENE outperforms existing methods in GRN inference tasks. It achieves optimal predictions on 90% of datasets in terms of area under the precision-recall curve (AUPRC) and optimal performance on 66.7% of datasets in terms of area under the receiver operating characteristic curve (AUROC). Compared to the latest methods, it shows an average improvement of 17.66% in AUPRC and 3.08% in AUROC. Additionally, we apply TDAGENE to analyze 3 key regulators (NANOG, SOX2, and POU5F1), revealing that incorporating topological information effectively captures critical features during cell fate specification. These findings highlight the potential of TDAGENE in inferring GRNs. - Source: PubMed
Publication date: 2026/05/05
Wu YufengKang YanleiGu JialiZhu HancanLi Zhong - Long interspersed nuclear element-1 (LINE-1, L1) shows high transcriptional activity in human pluripotent stem cells (PSCs). Here, we showed that pluripotency-associated transcription factors (TFs) such as POU5F1 (OCT4), SOX2, KLF4, NANOG, and MYC bind to the 5' untranslated region of full-length L1 copies of young subfamilies (L1HS, L1PA2, L1PA3), with their respective consensus sequences containing their binding motifs. These sites display features of open chromatin, H3K4me3 enrichment, and ATAC-seq signals, suggesting potential transcriptional activation. Notably, knockdown of POU5F1 led to increased L1 expression in independent experiments, suggesting that POU5F1 restricts L1 activity in PSCs. Similar trends were observed for SOX2 and NANOG, although supporting mRNA-seq datasets were limited. ChIP-seq analyses revealed binding of POU5F1, SOX2, NANOG, KLF4, and MYC to the 5' UTR of younger L1 subfamilies. Motif analysis revealed that binding sequences for these TFs are also conserved in older L1 subfamilies, suggesting a role for these TF binding in host fitness and/or L1 amplification. Together, our findings support a model in which pluripotency-associated TFs such as POU5F1 preferentially bind to transcriptionally competent L1 loci and modulate L1 expression in human PSCs, although the extent of their regulatory roles may differ among the TFs. - Source: PubMed
Suzuki HikaruIchiyanagi Kenji - Pluripotent stem cells (PSCs) exist in either a "primed" state or a "naive" state. While several protocols are available to convert primed human PSCs (hPSCs) to the naive-state hPSCs, they often require multiple exogenous factors. Here, we show that the activation of AMP-activated protein kinase (AMPK) or its downstream p38 alone induces naive conversion. Primed hPSCs cultured with activated AMPK-p38 displayed key naive features, including naive marker expression, three-germ-layer differentiation, epigenomic resetting, and increased mitochondrial activity. An AMPK activator-5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR)-synergistically enhances the naive conversion efficiency of reported conversion protocols. Single-cell RNA sequencing (scRNA-seq) with RNA velocity analyses and Totem trajectory mapping identified an intermediate state bridging the primed and naive states. These cells showed three upregulated gene groups: (1) pluripotency genes (e.g., and ), (2) naive state-related genes (e.g., and ), and (3) differentiation-suppressive genes (e.g., and ). These findings establish a simple induction method that illuminates underlying mechanisms and enables broad applications through efficient naive conversion. - Source: PubMed
Publication date: 2026/04/01
Yang ZhennanLiu YajingXu HuaigengYamane JunkoHotta AkitsuFujibuchi WataruYamashita Jun K - The effectiveness of treatment of head and neck squamous cell carcinomas (HNSCC) is still unsatisfactory, and novel therapeutics could improve outcomes. Histone deacetylases (HDAC) and histone lysine demethylases (KDMs) emerged as important molecular targets in HNSCC. Moreover, joint inhibition of epigenetic targets may be therapeutically advantageous. Thus, the aim of this project was to evaluate the effects of combinations of panobinostat, a pan-HDAC inhibitor, with KDM4-6 inhibitors (KDMi), ML324, GSK-J4, and JIB-04. Experiments were performed in FaDu and SCC-152 cell lines. Resazurin and clonogenic assays were used to evaluate the cell viability and clonogenic potential, respectively. Apoptosis was assessed by flow cytometry after Annexin V staining. Flow-cytometric detection of γH2A.X was applied for DNA damage evaluation. Gene expression was quantified by qPCR. KDM proteins occupancy at gene promoters was measured by quantitative chromatin immunoprecipitation. KDMi enhanced the anticancer effects of panobinostat in HNSCC cell lines. The combinations of panobinostat with ML324 and JIB-04 synergistically reduced cell viability in FaDu and SCC-152 cells, and increased apoptosis induction in SCC-152 cells. These effects could be attributed to the modulation of BIRC5 and CDKN2A expression, and enhanced accumulation of DNA double-strand breaks following combinatorial treatments in FaDu cells. Decreased expression of stemness-related genes upon KDMi treatment in FaDu cells was associated with decreased binding of KDM4A and/or KDM6B at SOX2 and POU5F1 gene promoters. The suppression of stemness-associated phenotype, and the concurrent promotion of apoptosis by the studied combinations of chemicals, suggest their potential as a novel therapeutic strategy in HNSCC. - Source: PubMed
Publication date: 2026/04/20
Dorna DawidKleszcz RobertDrabarz KarolinaKubiak MałgorzataStefanska BarbaraPaluszczak Jarosław