SOX9 polyclonal antibody
- Known as:
- SOX9 pab (anti-)
- Catalog number:
- PAB18221
- Product Quantity:
- 100 ug
- Category:
- -
- Supplier:
- Abno
- Gene target:
- SOX9 polyclonal antibody
Related genes to: SOX9 polyclonal antibody
- Gene:
- SOX9 NIH gene
- Name:
- SRY-box 9
- Previous symbol:
- CMD1, CMPD1
- Synonyms:
- SRA1
- Chromosome:
- 17q24.3
- Locus Type:
- gene with protein product
- Date approved:
- 1992-09-25
- Date modifiied:
- 2018-06-25
Related products to: SOX9 polyclonal antibody
Related articles to: SOX9 polyclonal antibody
- Astrocytes play essential roles in neuronal development, function, and disease, yet existing methods to derive astrocytes from human pluripotent stem cells (hPSCs) are complex and can involve months of maturation. We developed a genomic safe-harbor knock-in system for inducible expression of the astrogenic transcription factors NFIA, NFIB, and SOX9, enabling rapid and robust generation of functional induced astrocytes (iAstrocytes). Across five hPSC lines, NFIB-SOX9 and NFIA-NFIB-SOX9 combinations efficiently generated highly pure populations expressing astrocyte-specific and synaptogenic genes. iAstrocytes displayed cytokine-induced expression of complement factors C3 and C4 and were amenable to CRISPR interference (CRISPRi) gene expression knockdown. Optimization of culture conditions enabled survival of NFIB-SOX9 iAstrocytes in co-culture with human induced neurons (iNeurons). Through pharmacological and genetic perturbations, we uncovered a previously undescribed phenomenon in which co-culture with iAstrocytes promoted the development of synchronized iNeuron network calcium activity mediated by specific gap junction proteins. This rapid and genetically tractable iAstrocyte platform provides a robust model to dissect human genetic and environmental effects on astrocyte-neuron interactions. - Source: PubMed
Publication date: 2026/06/05
Morshed NaderDemers MatthewGonzalez-Ramos AnaJäntti HennaDoman JordanD'Souza SeanLi LetianGranger Adam JJohnson Matthew BStevens Beth - The zig-zag eel () exhibits sexual dimorphism in growth patterns. Identifying the genes involved in sex differentiation is a crucial step toward achieving single-sex breeding and serves as a vital foundation for elucidating the XY sex determination mechanism in . This study measured the morphological characteristics of male and female and found that males were significantly superior to females in body weight and nearly all morphological indices. Subsequently, whole-transcriptome sequencing was performed on the gonads of adult males and females, identifying 11,714 DEmRNAs, 3442 DElncRNAs, 416 DEcircRNAs, and 620 DEmiRNAs, including male sex differentiation genes such as , , , and , and female sex differentiation genes like , , and . Functional enrichment analysis identified pathways associated with sex differentiation, including the TGF-beta signaling pathway, the steroid hormone biosynthesis, the Hippo signaling pathway, and the Wnt signaling pathway, etc. A ceRNA network was constructed based on differentially expressed mRNAs and ncRNAs, revealing that the sex differentiation-related genes , , , , and are regulated by one or multiple pairs of lncRNA/circRNA-miRNA pairs. The study results will provide molecular targets for research on sex-controlled breeding in and lay an important theoretical foundation for clarifying its sex differentiation mechanisms. - Source: PubMed
Publication date: 2026/06/05
Zhu JunxianJia XianghuiJi LiqinChen ChenGao CaixiaHong XiaoyouLiu XiaoliWei ChengqingZhu XinpingLi Wei - Differentiating mesenchymal stromal cells into healthy chondrocytes for articular cartilage requires a variety of biochemical and biomechanical stimuli. While the effects of such stimuli on chondrogenic markers are well-studied, no bioreactor has thus far collected real-time data from mRNA expression for use in closed-loop control. Here, we present the design of an automated bioreactor that applies perfusion, oscillating hydrostatic pressure, and varying concentrations of multiple biochemical growth factors to the cell cultures while measuring fluorescent markers of gene expression in real-time. Our system achieves a gradated hydrodynamic environment across the length of cell cultures with flow rates between 0.46 and 3.2 cm s-1 and shear stresses between 5.0 and 60 mPa, can apply oscillating hydrostatic pressure at up to 0.5 Hz and 4.83 MPa, and can dynamically control the concentrations of two biochemical growth factors. The integrated fluorescent fiberscope system can measure fluorescent dye concentrations as low as 3 nᴍ within the cell chamber. Our LNA/DNA nano-biosensor is designed to track Sox9 mRNA, a gene critical to the chondrogenic process. The reactor is controlled via MATLAB Simulink and includes remote observation and control features to increase flexibility and minimize downtime. This novel bioreactor provides a platform for further articular cartilage research with the ultimate design goal of generating a transfer function that maps growth factor inputs to mRNA expression outputs for real-time control. - Source: PubMed
Publication date: 2026/06/11
Schuler Alec WilliamRobertson TerreillHart Stephanie SharayBarrow EricKallish NathanPhondee PakkanpatDriskell RyanBonassar LawrenceDong Wen-JiVan Wie Bernard JGozen Bulent Arda - Human endometrium sheds and regenerates each month during the menstrual cycle. N-cadherin (CDH2) glandular epithelial progenitors and SUSD2 mesenchymal stem cells (MSCs) and their niches have been identified, but their signaling interactions remain unknown. SSEA1 epithelial cells resurface the endometrium, generating a new luminal epithelium each cycle. Using these markers, we characterized the gene expression of human endometrial stem/progenitor cell-enriched populations derived from fluorescence-activated cell sorting (FACS)-sorted hysterectomy endometrium by single-cell RNA sequencing (scRNA-seq). Two of 10 epithelial clusters contained CDH2SOX9 cells with high TRH and IHH expressions. N-cadherin and IHH, and SSEA1 and Hedgehog co-receptor BOC immunoco-localized in the basal layer of endometrial glands, from which the new functional layer glands regenerate each menstrual cycle. Two of six mesenchymal clusters contained SUSD2 MSCs, one with high MUSTN1 expression. Epithelial progenitors and endometrial MSCs transitioned to their respective progeny. We provide new insights into the human endometrial stem/progenitor cell signaling pathways and niche interactions regulating their function. - Source: PubMed
Publication date: 2026/06/11
Fitzgerald Harriet CMortlock SallyFilby Caitlin ECousins Fiona LMarquez-Garcia ElizabethWyatt Katherine AMcKinnon BrettRombauts LukTsaltas JimMontgomery Grant WGargett Caroline E - The aim of this study is to reveal the molecular mechanisms of BSYJF in KOA treatment through bioinformatics, animal and cellular experiments. - Source: PubMed
Publication date: 2026/06/01
Zhai Yu-QingLu Jian-WeiYan QiangDu Jia-WeiGe Yang-ShuoZhao Min-JunYin Jian-LiHuang Chun-MengMeng Ting-TingHuang Xin-HuiChen Liao-LinLuo Jia-HuiWang Xue-ZongDing Daofang