PRDX1 monoclonal antibody, clone 2A4
- Known as:
- PRDX1 mab (anti-), clonality 2A4
- Catalog number:
- MAB0713
- Product Quantity:
- 100 uL
- Category:
- -
- Supplier:
- Abno
- Gene target:
- PRDX1 monoclonal antibody clone 2A4
Related genes to: PRDX1 monoclonal antibody, clone 2A4
- Gene:
- PRDX1 NIH gene
- Name:
- peroxiredoxin 1
- Previous symbol:
- PAGA
- Synonyms:
- NKEFA
- Chromosome:
- 1p34.1
- Locus Type:
- gene with protein product
- Date approved:
- 1993-11-01
- Date modifiied:
- 2014-11-19
Related products to: PRDX1 monoclonal antibody, clone 2A4
Related articles to: PRDX1 monoclonal antibody, clone 2A4
- Breast cancer remains the most common cancer among women and a major cause of mortality. Fisetin, a natural compound, has demonstrated anti-cancer activity, but its limited bioavailability reduces its therapeutic effect. To overcome this, glucose-capped fisetin silver nanoparticles (GF-Ag NPs) were synthesised, and their significant impact on the MDA-MB-231 breast cancer cell line is reported. This study focused on the proteomic profiling of GF-Ag NP-treated MDA-MB-231 cells using two-dimensional gel electrophoresis and mass spectrometry, which identified 25 proteins with altered expression. Gene ontology and protein-protein interaction analyses indicated these proteins are involved in critical biological and molecular processes. Four key proteins-Nucleolin (NCL), Tropomyosin alpha-4 chain (TPM4), L-lactate dehydrogenase A (LDHA), and Peroxiredoxin-1 (PRDX1)-were chosen for further validation through qRT-PCR based on their relevance to cancer progression. Additionally, bioinformatic prediction tools identified several miRNAs (such as miR-577, miR-205-5p, miR-613, miR-206, and miR-374b-5p) and associated lncRNAs as regulators of these proteins. These results suggest that GF-Ag NPs can modulate the expression of key genes and signalling pathways involved in breast cancer progression and apoptosis. - Source: PubMed
Publication date: 2026/07/04
Subhalakshmi KVeeraraghavan Vishnu PriyaSivagnanam AnanthiThangasamy BalasankarFrancis Arul Prakash - Hexavalent chromium (Cr[VI]) is a significant environmental contaminant that poses substantial risks to agricultural safety and public health. Although Cr(VI)-induced hepatic injury is associated with oxidative stress and organelle dysfunction, the crosstalk between ionic homeostasis disruption and organelle-specific pathophysiology remains incompletely elucidated. Herein, we identified significant suppression of peroxiredoxin 1 (PRDX1) following Cr(VI) exposure, concomitant with intracellular calcium depletion, mitochondrial lipid peroxidation, endoplasmic reticulum (ER) stress, and ferroptosis. Mechanistically, Cr(VI)-driven PRDX1 deficiency initiates calcium-mediated mitochondrial oxidative damage and ER stress, synergistically triggering the ferroptosis cascade that culminates in hepatic injury. These findings establish PRDX1 as the master regulator of Cr(VI) hepatotoxicity and uncover a novel calcium-dependent axis that converges mitochondrial redox imbalance with ER proteotoxic stress to drive ferroptotic cell death. This study provides fundamental insights into the toxicological role of PRDX1 in Cr(VI) exposure and advances theoretical frameworks for understanding chromium-associated pathophysiological mechanisms. - Source: PubMed
Publication date: 2026/07/03
Han MingzhengWu ZiqiGuo ShuaihaoChen BohaoWang JingchunYu JinglingHuang HaiGuo JianyingTang ZhaoxinHuang HuiLiao Jianzhao - The search for molecular containers that enhance the bioavailability of active substances has opened the development of functionalized βCD-based molecular containers. In this study, the effects of βCD-modified with dimethylolpropionic acid (Bis-MPA) dendrons (βCD-m2G and βCD-m3G) were evaluated in SVGp12 (non-tumor astrocytes) and A172 (glioblastoma) cells. The dendronized systems exhibited low cytotoxicity (>75% viability) even at high concentrations (90 μM, p < 0.05). Morphological analyses revealed lipid inclusion bodies (LIBs), suggesting that after intracellular uptake, the dendronized containers promote their formation. βCD-m3G occupies 12% of the cell volume in SVGp12 and 17% in A172 (p < 0.05). Ultrastructural evaluation showed increased smooth endoplasmic reticulum (SER), a finding consistent with enhanced lipid metabolism. Importantly, cell differentiation and endoplasmic reticulum stress, evaluated by the expression of Aquaporin-4 and calnexin, were not impaired (p > 0.05). A twofold increase in mGlu-5 expression (p < 0.001) was observed in both cell lines due primarily to the βCD-m3G container. Because neither container induces oxidative stress in the SVGp12 cell line, cell proliferation increases from 41% to 48% (p < 0.001) due to βCD-m2G and βCD-m3G, respectively. These cells also increased Caspase9 expression (p < 0.001), suggesting control of proliferation. However, βCD increased NRF2 expression by 4.3-fold (p < 0.0001) and the expression of SOD1, HMOX1, and PRDX1. βCD increases 1.8-fold-ROS in A172 cells (p < 0.001) versus control. 3.5-fold expression of NRF2 was observed in the presence of NAC (p < 0.001). These suggest that only βCD increases NRF2 and ROS as an adaptive response of A172 cells. The incorporation of amphiphilic dendritic groups enables efficient cellular uptake of βCD-dendronized containers. - Source: PubMed
Publication date: 2026/06/30
Cabrera-Quiñones Neyra CitlaliRoldán-Barreto ElisaZúñiga-Castellanos RosalíaZaragoza-Ojeda MontserratMares-Muñoz JuanLedesma-Beiza AdriánVargas-Hernández JacobJiménez-García Luis FelipeRojas-Aguirre YareliArenas-Huertero FranciscoGuadarrama Patricia - In this study, integrated proteomic and transcriptomic analyses identified peroxiredoxin 1 (PRDX1) as a novel urinary biomarker for bladder cancer (BC). PRDX1 was significantly upregulated in BC tissues and was associated with poorer overall survival. In vitro experiments further demonstrated that PRDX1 promotes malignant phenotypes of BC cells, including proliferation, migration, and invasion. Silencing PRDX1 in BC cells significantly reduced the invasiveness and proliferation ability.To address the clinical need for rapid and non-invasive detection, we developed an innovative optical fiber biosensor based on surface plasmon resonance (SPR) technology for the quantitative detection of urinary PRDX1. The biosensor exhibited excellent analytical performance, including high sensitivity (limit of detection: 0.06 ng/mL), a wide linear range (0-25 ng/mL), rapid response (∼14 s), as well as good stability and selectivity. In clinical validation involving 97 BC patients and 30 healthy controls, the biosensor demonstrated outstanding diagnostic performance, with an area under the receiver operating characteristic curve (AUC) of 0.91 and an overall diagnostic accuracy of 86.6%, outperforming conventional enzyme-linked immunosorbent assay (ELISA). Collectively, this study not only identifies PRDX1 as a promising biomarker for non-invasive diagnosis and prognostic evaluation of BC, but also establishes an efficient SPR-based optical fiber sensing platform, providing new insights into both clinical detection and the functional role of PRDX1 in BC progression. - Source: PubMed
Publication date: 2026/06/15
Cheng KunZhang Heng-BiaoXie Hua-RongWang LiZhang TongWan ShunYang Jian-WeiHong Pan-GuanWang Xu-YanJiao Pan-PanXu Chang-HongDeng Yi-diChen Si-YuDing Li-YunYang Li - Within the scope of this investigation, two novel compounds (3a and 3b) were designed and synthesized in two steps. Compounds 3a and 3b were tested utilizing the MTT study to evaluate their in vitro cytotoxic activity against healthy human embryonic kidney, lung cancer, breast cancer, and human liver cancer cell lines. It was determined that compound 3b exhibited high levels of cytotoxic activity against both liver and breast cancer cell lines, with IC values of 10.83 and 11.55 µM, respectively. Also, to elucidate the anticancer mechanism of compounds, pro-apoptotic BAX and BiD, anti-apoptotic BCL2 and BCL-xl, oxidant enzymes PRDX1 and SOD1 levels were examined by RT-qPCR. Moreover, cellular oxidative stress levels were spectrophotometrically measured, and cellular senescence was evaluated via the senescence-associated β-galactosidase test. DFT calculations and RDG, ELF, and LOL analyses were performed to demonstrate the reactivity of these compounds. Compounds significantly down-regulated anti-apoptotic genes, whereas mRNA levels of pro-apoptotic genes were up-regulated in MCF-7 cells. Moreover, oxidative stress status was significantly increased depending on the compound treatment. Consistently, PRDX1 and SOD1 levels were also significantly up-regulated. Furthermore, cellular senescence was significantly induced by the compounds. Fluorescence spectroscopy demonstrated strong binding of both compounds with CT-DNA, characterized by static quenching mechanism and binding constants of 6.02 × 10M(for 3a) and 8.69 × 10M(for 3b), suggesting an intercalative binding mode. In contrast, moderate affinity toward BSA indicated suitable transport characteristics with reduced nonspecific protein binding. Molecular docking studies supported the experimental findings. These combined results verify the potential of the synthesized derivatives as promising candidates for further investigation as biologically active anticancer agents. - Source: PubMed
Publication date: 2026/06/23
Kundu SevgiAkkoc SenemErzurumlu YalçınAlhag Sadeq KAlkeridis Lamya AhmedFeizi-Dehnayebi Mehran