TRAY, BOTTLE, WASH, 2-PS
- Known as:
- TRAY, BOTTLE, WASH, 2-PS
- Catalog number:
- rvdia04620118304
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Diasource
- Gene target:
- TRAY BOTTLE WASH 2-
Related genes to: TRAY, BOTTLE, WASH, 2-PS
- Gene:
- WASHC1 NIH gene
- Name:
- WASH complex subunit 1
- Previous symbol:
- FAM39E, WASH1
- Synonyms:
- FLJ00038
- Chromosome:
- 9p24.3
- Locus Type:
- gene with protein product
- Date approved:
- 2007-08-02
- Date modifiied:
- 2016-10-14
Related products to: TRAY, BOTTLE, WASH, 2-PS
1 x 175-225ml Bottle Adapters For 250ml Round Buckets1 x 400ml Bottle Adapters For 500ml Rectangular Buckets1-Drop PCR Mix (squeeze bottle, no pipet)1-Drop PCR Mix (squeeze bottle, no pipet)1-Drop PCR Mix (squeeze bottle, no pipet)10 x 10cm UV Gel Tray For Midi Horizontal Gel Box10 x 7cm UV Gel Tray For Midi Horizontal Gel Box1000ml Square Media bottle for Media serum bottling or casual uses 12/tray Sterile to SAL 10-61000ml Square Media bottle for Media, serum bottling or casual uses 12_tray, Sterile to SAL 10-6, 24_case1000ml Square Media bottle for Media, serum bottling or casual uses 12_tray, Sterile to SAL 10-6, 24_case box size(mm):310x400x4651000UL TRAY ASSY 1ml 60 WELL100ml bottle for C0320-720 insert, ea.100ml bottle for C0320-720 insert, ea.100ml bottle for C0320-720 insert, ea.10x ELISA WASH BUFFER Accessory Reagent Host: N_A Related articles to: TRAY, BOTTLE, WASH, 2-PS
- WNT2B is canonically characterized as a secreted WNT-family ligand, which is transported to the extracellular space via the endoplasmic reticulum (ER)-Golgi pathway and binds to cell surface FZDs (frizzled class receptors) to trigger downstream signaling cascades. Here, we identify a previously unrecognized non-secretory intracellular function of WNT2B in impairing endosomal trafficking to inhibit macroautophagy/autophagy, as well as a non-canonical LC3B-II-dependent autophagic secretion mechanism for WNT2B. Specifically, the non-secretory intracellular pool of WNT2B via its conserved middle domain (MD) binds to the spectrin repeat domain (SRD) of WASHC5, competitively displacing WASHC1 and thereby disrupting WASH complex assembly and inhibiting WASHC1-mediated actin polymerization on early endosomes. This disruption impairs endosomal cargo trafficking, including the core autophagy protein ATG9A, leading to defective autophagy initiation and subsequent accumulation of pro-inflammatory and pro-fibrotic factors in fibroblasts. We validated this mechanism in vivo using a TNBS-induced mouse model of chronic colitis. Fibroblast-specific deletion restores autophagy, reduces pro-inflammatory cytokine secretion, and ameliorates intestinal fibrosis. Consistently, in Crohn disease (CD) patient tissues, elevated WNT2B in fibrotic regions negatively correlates with autophagy activity, and positively correlates with pro-fibrotic phenotypes, and clinical disease severity. Moreover, we identify a novel LC3B-II-dependent autophagic secretion pathway for WNT2B, which is distinct from the conventional ER-to-Golgi-dependent protein secretion. Collectively, our study delineates a novel non-canonical WNT2B-WASH complex-ATG9A regulatory axis through which WNT2B impairs endosomal trafficking and disrupts autophagy, ultimately amplifying inflammation and fibrosis. This study suggests that WNT2B may serve as a promising therapeutic target for CD and autophagy-associated fibrotic disorders.: 3-MA: 3-methyladenine; AAV: adeno-associated virus; ACTA2: actin alpha 2, smooth muscle; ARPC2: actin related protein 2/3 complex subunit 2; ATG: autophagy related; CCN3: cellular communication network factor 3; CD: Crohn disease; CK666: 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide; COL1A1: collagen type I alpha 1 chain; Co-IP: co-immunoprecipitation; CTNNB1: catenin beta 1; DBcAMP: dibutyryl cyclic adenosine monophosphate; DPT: dermatopontin; EEA1: early endosome antigen 1; EGFR: epidermal growth factor receptor; ELISA: enzyme-linked immunosorbent assay; ER: endoplasmic reticulum; ESCRT: endosomal sorting complexes required for transport; EV: extracellular vesicle; FRAP: fluorescence recovery after photobleaching; FL: full length; FZD: frizzled class receptor; GST: glutathione S-transferase; HIF: human intestinal fibroblast; HMGB1: high mobility group box 1; IKBKB: inhibitor of nuclear factor kappa B kinase subunit beta; IL6: interleukin 6; LDELS: LC3-dependent EV loading and secretion; LPS: lipopolysaccharide; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MD: middle domain; MEFs: mouse embryonic fibroblasts; MTOR: mechanistic target of rapamycin kinase; MVB: multivesicular body; NFKB: nuclear factor kappa B; NFKBIA: NFKB inhibitor alpha; PDCD6IP: programmed cell death 6 interacting protein; PLA: proximity ligation assay; RELA/p65: RELA proto-oncogene, NF-kB subunit; SAFB: scaffold attachment factor B; SES-CD: Simple Endoscopic Score for Crohn disease; SIM: super-resolution structured illumination microscopy; SMAD3: SMAD family member 3; SQSTM1/p62: sequestosome 1; SRD: spectrin repeat domain; TEM: transmission electron microscopy; TFRC: transferrin receptor; TGFB1: transforming growth factor beta 1; TGOLN2: trans-golgi network protein 2; TNBS: 2,4,6-trinitrobenzenesulfonic acid; TNF: tumor necrosis factor; VCA: Verprolin homology, Central and Acidic; WASHC: WASH complex subunit; WLS: Wnt ligand secretion mediator; WCL: whole cell lysates; WNT: Wnt family member; WT, wild type. - Source: PubMed
Publication date: 2026/06/03
Liu DanqiongCheng YanlingHuang ChuxiangXie KangJie JiananZhang QingqingLan LinChen PeiyuXie JingWang HongliRen LuLi HuiwenGeng LanlanGong SitangZhu YunCheng Yang - Prolactinomas are the most common functional pituitary adenomas, and dopamine agonists (DAs) are the first-line therapy; however, approximately 10-30% of patients develop resistance, highlighting the need for effective sensitization strategies. In clinical specimens, we observed reduced p300 expression in tumors with poor DA responsiveness, and p300 levels were inversely associated with DA dosage. In cellular and xenograft models, DAs decreased p300 by suppressing the cAMP/PKA/CREB pathway. We therefore tested whether upregulating or activating p300 could enhance DA efficacy and investigated the underlying mechanism using immunohistochemistry, immunofluorescence, Western blot, genetic manipulations, RNA sequencing, CUT&Tag, ChIP-qPCR, Seahorse metabolic assays, flow cytometry, co-immunoprecipitation, and GST pull-down assays. Augmenting p300 markedly potentiated DA-induced antitumor effects in vitro and in vivo, a process accompanied by the elevated histone H3K18 lactylation (H3K18la). Mechanistically, p300-dependent H3K18la promoted transcriptional upregulation of Ndufs7 and Washc1. NDUFS7 induction was associated with increased mitochondrial ROS, whereas WASH1 bound the ubiquitin-associated domain of p62, impairing recognition and clearance of damaged mitochondria, suppressing mitophagy, and thereby sustaining mitochondrial ROS accumulation and apoptosis. Moreover, YF-2, a p300 HAT-domain activator, synergized with DAs to inhibit tumor growth in MMQ and AtT-20 cells. Together, these data identify a p300-H3K18la-NDUFS7/WASH1 axis that links mitophagy inhibition to mitochondrial ROS accumulation and provide a mechanistic rationale for targeting p300 as an adjuvant approach to improve DAs efficacy in prolactinomas. - Source: PubMed
Publication date: 2026/02/11
Li SihanJiang QianWang QuanjiLi XingboWang ZihanXu LinpengLuo ShuyanWang YaoruiZhang HuaqiuShu KaiLei TingHuang YiminLei Zhuowei - During infection with the cytomegalovirus (CMV), the membrane system of the infected cell is remodelled into a megastructure called the assembly compartment (AC). These extensive changes may involve the manipulation of the host cell proteome by targeting a pleiotropic function of the cell such as ubiquitination (Ub). In this study, we investigate whether the Ub system is required for the establishment and maintenance of the AC in murine CMV (MCMV)-infected cells Methods: NIH3T3 cells were infected with wild-type and recombinant MCMVs and the Ub system was inhibited with PYR-41. The expression of viral and host cell proteins was analyzed by Western blot. AC formation was monitored by immunofluorescence with confocal imaging and long-term live imaging as the dislocation of the Golgi and expansion of Rab10-positive tubular membranes (Rab10 TMs). A cell line with inducible expression of hemagglutinin (HA)-Ub was constructed to monitor ubiquitination. siRNA was used to deplete host cell factors. Infectious virion production was monitored using the plaque assay. - Source: PubMed
Publication date: 2025/07/31
Radić BarbaraŠtimac IgorOmerović AlenViduka IvonaMarcelić MarinaZagorac Gordana BlagojevićLučin PeroLučin Hana Mahmutefendić - Polyhexamethylene guanidine (PHMG) is widely utilized in personal hygiene products due to its bactericidal, non-volatile, and hydrophilic properties. However, the long-term toxic effects and underlying mechanisms associated with respiratory exposure to the commonly used form, PHMG phosphate (PHMG-p), are still insufficiently understood. This study aims to elucidate the types of pulmonary lesions and the incidence of lung cancer associated with varying concentrations of PHMG-p and observation periods, along with the molecular mechanisms underlying this relationship. To assess these effects, CT scans and pathological analyses were conducted for up to 54 weeks following initial exposure to PHMG-p. Furthermore, to investigate the underlying causes of pulmonary toxicity, TGF-beta-activated kinase 1 was identified as a PHMG-p-binding protein, and its associated signaling pathways, including necroptosis, apoptosis, and MKK7, were explored. Somatic mutational signature, and gene ontology (GO) analyses were performed to investigate the genetic characteristics of PHMG-p-induced lung carcinogenesis. PHMG-p exposure led to somatic mutations in lung cancer-related genes, including TP53, SOS1, KMT2D, MDM2, ERBB2, SETD2, MET, ARID1A, RBM10, and CDKN2A as well as in genes such as RAB31, WASHC1, DDX11, ECD, STAB2, MUC2, and MUC5AC. The mutated genes were primarily associated with impaired DNA repair mechanisms. GO analysis highlighted the activation of pathways related to cell cycle checkpoints, necroptosis, MAPK, and idiopathic pulmonary fibrosis, while also revealing the suppression of signaling pathways associated with natural killer cells, GADD45, LXR/RXR activation, and IL-15 production. Gain-of-function experiments confirmed the oncogenic roles of PLAU and HMGA2, as well as the tumor-suppressive functions of TBX4 and GPX3. These findings suggest that PHMG-p activates necroptosis and MAPK signaling, increases the frequency of somatic mutations, and inhibits apoptosis, thus fostering an environment conducive to carcinogenesis. This underscores the importance of understanding the potential health risks associated with PHMG-p exposure and provides insights for future research and regulatory considerations regarding the safety of personal hygiene products. - Source: PubMed
Publication date: 2024/11/24
Lee HongJeong Sang HoonBaek Yong-WookLee HyejinSa Jason KLee Ji YoonLee Yu-SeonNam Yoon JeongKim JaeyoungKim JonghoonChoi Jin YoungPark Su AKim Je HyeongPark Yoon HeeLim JungyunKim Young-HeePark Eun-KeeKim CherryLee Ju-Han - The recently published T2T-CHM13 reference assembly completed the annotation of the final 8% of the human genome. It introduced 1956 genes, close to 100 of which are predicted to be coding because they have a protein coding parent gene. Here, we confirm the coding status and functional relevance of two of these genes, paralogues of and . We find that , one of four novel subtelomeric WASH1 genes uncovered in the new assembly, produces the WASH1 protein that forms part of the vital actin-regulatory WASH complex. Its coding status is supported by abundant proteomics, conservation, and cDNA evidence. It was previously assumed that gene produced the functional WASH1 protein, but new evidence shows that is a human-derived duplication and likely to be one of 12 WASH1 pseudogenes in the human gene set. We also find that the T2T-CHM13 assembly has added a functionally important copy of to the human gene set. We demonstrate that uniquely mapping peptides from proteomics databases support the novel rather than the GRCh38 assembly gene. These new additions to the set of human coding genes underlines the importance of the new T2T-CHM13 assembly. - Source: PubMed
Publication date: 2024/02/28
Cerdán-Vélez DanielTress Michael Liam