MIS12 antibody
- Known as:
- MIS12 (anti-)
- Catalog number:
- orb100626
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Biorbyt biorb
- Gene target:
- MIS12 antibody
Related genes to: MIS12 antibody
- Gene:
- MIS12 NIH gene
- Name:
- MIS12 kinetochore complex component
- Previous symbol:
- -
- Synonyms:
- MGC2488, hMIS12, KNTC2AP, MTW1
- Chromosome:
- 17p13.2
- Locus Type:
- gene with protein product
- Date approved:
- 2004-07-29
- Date modifiied:
- 2019-01-25
Related products to: MIS12 antibody
Related articles to: MIS12 antibody
- Accurate kinetochore-microtubule (K-MT) attachment is crucial for chromosome segregation in oocyte meiosis. The NDC80 complex, a subcomplex of the outer kinetochore KNL1‑MIS12‑NDC80 (KMN) network, is canonically responsible for K‑MT attachment. Here, by employing a conditional knockout (cKO) mouse model, combined with protein localization analysis for MIS12 following chromosome spreading, we demonstrate that endogenous MIS12 localizes to kinetochores in both mouse and human oocytes, and depleting MIS12 severely disrupts K-MT attachment in both meiotic divisions, independent of outer kinetochore protein NDC80. Crucially, we identified that MIS12 directly interacts with β-tubulin (including TUBB3 and TUBB5), which is essential for microtubule attachment to kinetochores, revealing a previously unknown mechanism of K-MT interaction. Furthermore, we definitively show that MIS12 is required for spindle assembly checkpoint (SAC) signaling by stabilizing the KNL1 assembly. Our findings not only resolve the previous controversy by establishing the canonical and essential role of kinetochore-localized MIS12 in oocytes but also redefine its molecular function through its direct binding to microtubules, providing a new paradigm for KMN network-mediated K-MT attachment in mammalian oocyte meiosis. - Source: PubMed
Publication date: 2026/06/24
Li JianZhang Chun-HuiWang YongLi Cheng-YuanWen Da-RongXia XiZhou LiangQian Wei-PingSun Qing-YuanLi Chang-Zhong - This study analyses Canis remains recovered from the Sierra de Atapuerca sites over the last 25 years. The sample comprises 108 dentognathic specimens: 106 attributed to C. mosbachensis from Sima del Elefante and Gran Dolina, and 2 to C. lupus from Galería. These remains document a record spanning the Early and Middle Pleistocene, approximately 1.2-0.4 Ma. The results indicate metrical stability in C. mosbachensis, which is consistently smaller than C. lupus. Specimens from the Early Pleistocene (TE9-TE14 and TD3-TD6) and the early Middle Pleistocene (TD8) exhibit more primitive morphological traits, particularly evident in the dentition of the carnassial-molar complex, consistent with mesocarnivory. In contrast, specimens from TD10 (middle Middle Pleistocene) tend to be larger than those from older stratigraphic levels and exhibit morphological features suggesting a gradual transition from C. mosbachensis to C. lupus. The lower carnassial in these individuals shows an elongation of the trigonid, indicating increased cutting efficiency and a shift toward a more carnivorous diet. This pattern likely represents an intermediate stage in this evolutionary transition rather than a fully developed hypercarnivorous condition comparable to that observed in modern wolves. Moreover, the morphological change observed in TD10 Canis coincides with a transition episode from MIS12 to MIS11, associated with a severe climatic shift. - Source: PubMed
Publication date: 2026/05/11
Blázquez-Orta RaquelSaladié PalmiraHuguet RosaOllé AndreuRodríguez-Hidalgo AntonioRodríguez-Álvarez Xosé PCáceres IsabelGarcía-Medrano PaulaSantos ElenaMartinón-Torres MaríaÁlvarez-Alonso Davidde Andrés-Herrero MaríaGarcía Nuria - Kinetochore proteins, long studied for their role in cell division, are also required for the proper postmitotic development of hippocampal and cortical neurons. Proteins of the kinetochore complex were present in axons and dendrites of postmitotic iNeurons where they resided, at least in part, at microtubule plus ends. Conditional deletion of mouse Ndc80 or Dsn1 increased the number of dendritic spines. Loss of any of three kinetochore components (Ndc80, Dsn1, or Mis12) increased microtubule plus-end dynamics. Observations of individual microtubules in indicated that Ndc80, the microtubule-binding component of the kinetochore complex, slowed the rate of microtubule growth. The increase in spine number in mammalian neurons correlated with increased microtubule invasion of spines. Both spine number and microtubule invasion phenotypes induced by Ndc80 deletion could be rescued by reexpression of Ndc80, but only if the microtubule-binding region of NDC80 was preserved. We propose that kinetochore proteins act in a complex resembling the mitotic kinetochore in order to stabilize microtubule plus ends and thereby restrain spine invasions and the development of dendritic spines. - Source: PubMed
Publication date: 2026/04/27
Zhao GuoliSharma AditiTang JingAleman MartinaLiang XingMiner LaurenQi JingqiXiang WangchuTian FengGoldberg YvesHe ZhigangShen KangPeris LeticiaL Schwarz Thomas - Gestational diabetes mellitus (GDM) is a common pregnancy complication linked to adverse outcomes, highlighting the need for new diagnostic markers. This study aimed to identify oxidative stress-related genes as potential biomarkers for GDM using integrated bioinformatics and experimental validation. - Source: PubMed
Publication date: 2026/03/06
He HaiyuWu Wen - Centromeres are comprised of long stretches of repetitive DNA that evolve rapidly in organisms across the tree of life. Consistent selfish centromere evolution can also have cascading effects - driving rapid evolution in interacting kinetochore proteins - possibly to maintain centromere-kinetochore compatibility. Effects of selfishly evolving centromeres on interacting proteins are most heavily studied in the inner kinetochore and assembly proteins including the constitutive centromere-associated network proteins CENP-A and CENP-C with some exploration of the extended effects to other kinetochore-associated protein complexes. While rapid evolution of the centromere has been broadly studied in many organisms, studies assessing positive selection in centromere-associated kinetochore proteins have largely focused on . Here, we tested the hypothesis that signatures of positive selection would be present in outer kinetochore and condensin genes in diverse animal groups. We selected two protein complexes - the Condensin I complex and the Mis12 Complex - to test for positive selection in parasitic wasps, two groups of ray-finned fishes (including the amazon molly an asexual diploid exempt from centromere drive), and two groups of primates. We did not find selection using any test in any protein in the amazon molly but did find sporadic positive selection in proteins in both complexes across all groups. - Source: PubMed
Publication date: 2026/02/16
Healey Hope MGomez L EnriqueSheikh Sofia ICamel Benjamin RForbes Andrew ASterner Kirstin NBeck Emily A