CDC2L1 antibody
- Known as:
- CDC2L1 (anti-)
- Catalog number:
- orb101250
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Biorbyt biorb
- Gene target:
- CDC2L1 antibody
Related genes to: CDC2L1 antibody
- Gene:
- CDK11B NIH gene
- Name:
- cyclin dependent kinase 11B
- Previous symbol:
- CDC2L1
- Synonyms:
- CDK11-p110, CDK11-p58, CDK11-p46
- Chromosome:
- 1p36.33
- Locus Type:
- gene with protein product
- Date approved:
- 1991-07-12
- Date modifiied:
- 2016-06-08
Related products to: CDC2L1 antibody
Related articles to: CDC2L1 antibody
- Alzheimer's disease is a multifactorial disease characterized by proteinopathy caused by senile plaques and neurofibrillary tangles, leading to neuroinflammation and neuronal damage. Bacopa monnieri, with its extensive usage in the Indian traditional medicinal system, Ayurveda, holds promise in enhancing memory and improving neuronal functions. We performed quantitative phosphoproteomics analysis to understand the temporal dynamics of protein phosphorylation involved in Bacopa monnieri-mediated neuroprotection in the Alzheimer's disease model of IMR-32 cells. The quantitative phosphoproteomics analysis identified 5488 phosphopeptides corresponding to 1427 phosphoproteins. Among the dysregulated peptides in the Aβ treatment group, 430 were significantly hyperphosphorylated, and 540 were significantly hypophosphorylated. In contrast, the Bacopa monnieri co-treatment group exhibited 64 significantly hyperphosphorylated peptides and 69 significantly hypophosphorylated peptides. The phosphoproteins altered by Aβ were involved in biological functions such as RNA splicing, neuron axonogenesis in the central nervous system, and positive regulation of tau-protein kinase activity. Bacopa monnieri restored key pathways altered by Aβ, including RNA metabolism, cellular response to stress, signaling by Rho GTPases, and RHOBTB2 GTPase cycle. Kinase mapping of differentially expressed phosphoproteins using the KinMap tool identified CDK11B, CDK12, PRKAA1, PRPF4B, STK10, ROCK1 and ROCK2 as downstream effectors of Bacopa monnieri. The network analysis of restored proteins revealed tight interactions between proteins associated with splicing regulation and stress response. This study offers a comprehensive understanding of phosphoproteome alterations involved in Alzheimer's disease in response to neuroprotection by Bacopa monnieri, which may pave the way for developing therapeutic strategies. - Source: PubMed
Publication date: 2026/09/29
Palollathil AkhinaNajar Mohd AltafPervaje RavishankarRaju RajeshKeshava Prasad Thottethodi SubrahmanyaModi Prashant Kumar - During early mammalian embryogenesis, totipotent zygotes and early blastomeres undergo extensive post-transcriptional regulation during the establishment of the first cell lineages; however, the functional contribution of alternative splicing to embryonic compaction and blastulation remains poorly understood. Here, we show that SF3B1, a core component of the spliceosome, is upregulated from the 4-cell stage and mediates highly dynamic splicing programs. Depletion of SF3B1 results in developmental arrest at the morula stage, accompanied by widespread transcriptomic dysregulation characterized by aberrant expression of transcription factors that impede pluripotency transition. Alternative splicing analysis further identifies that aberrantly spliced transcripts were significantly enriched in genes involved in cell cycle regulation, such as Cdk11b and Ccnb1. Importantly, we demonstrate that SF3B1 undergoes intrinsic, IDR-driven liquid-liquid phase separation both in vitro and in vivo, forming nuclear condensates in oocytes and early embryos, which is essential for successful development to the blastocyst stage. Together, our findings reveal that phase separation mediated SF3B1 splicing activity is a critical regulator of early mouse embryonic development. - Source: PubMed
Publication date: 2026/09/08
Zhao KangHan Ting-YuCheng Yan-LiZhang Yi-DanZhang Yu-WeiGuo JinJianZhang ShaoJunHuang WenZeZhang JingLiao Pei-YuXin YingChu ChuanChenSun Qing-YuanLiu ZhiZhenOu Xiang-HongXie Jun - Dual-specificity protein kinase CLK4 plays a pivotal role in regulating alternative mRNA splicing, DNA repair, and various cellular processes through precise phosphorylation. In this study, we analyzed 3825 global human cellular phosphoproteome studies, identifying 430 qualitative profiles and 55 quantitative differential datasets featuring high-confidence Class-1 phosphosites (localization probability ≥ 75%; A-score ≥ 13). Notably, S136 and S138 emerged as predominant phosphorylation sites outside the kinase domain. These sites showed frequent detection and differential expression in liver, lung, and head and neck cancers, as documented in PhosphositePlus. We identified a high-confidence set of co-regulated phosphoproteins, including SQSTM1, SRRT, RPS6, TP53BP1, TNKS1BP1, THUMPD1, OTUD4, and TCEA1. These proteins link CLK4 to critical pathways, including RNA splicing, autophagy, DNA damage response, and cancer progression. Binary interactors, including SRRM2, Interacts with SPT6 1 (IWS1), RBBP6, ZC3H18, BUD13, and DYRK1A, further connect CLK4 to RNA processing and splicing. Predicted downstream substrates, such as CCNL2, CDK11B, PRDX6, YTHDC1, RBM15, SRRM1, SFSWAP, HNRNPU, and DOCK7, highlight CLK4's broad regulatory scope. Upstream kinases were also predicted for S136 and S138. Site-resolved analyses revealed tumor-specific dysregulation of CLK4 phosphorylation at key residues. Co-occurring phosphosite alterations and nearby somatic mutations suggest disrupted CLK4 regulation in cancer. Overall, this study provides a comprehensive phosphoproteomic resource that maps CLK4's co-regulatory networks, paving the way for mechanistic investigations and targeted cancer therapies. - Source: PubMed
Publication date: 2026/07/22
Pai ApoorvaDcunha LeonaGopalakrishnan Athira PerunellyUmmar SamseeraRajeev Athira CRaju Rajesh - Gene-wise intratumor heterogeneity (ITH), defined as spatial variability in the expression of individual genes across tumor regions, remains incompletely characterized in non-small cell lung cancer (NSCLC). Identifying low-ITH genes as predictive biomarkers offers a promising strategy to enhance the reliability of immunotherapy outcome prediction. - Source: PubMed
Publication date: 2026/06/20
Yuan JianyeWeng ZelinLi ZhenguoChen RuiCheng ChaoYang WeixiongXie XiuyingLuo ChangWang TaoZhang ShuishenTan Zihui - Rhabdoid tumor of the kidney (RTK) is a highly aggressive pediatric malignancy characterized by biallelic loss, resulting in aberrant MYC pathway activation and cell cycle regulation. MYC-activated tumors are vulnerable in splicing functions and sensitive to splicing inhibitors. Therefore, in this study, cyclin-dependent kinase 11 (CDK11), which regulates both cell cycle and RNA splicing, was tested as a therapeutic target in RTK. - Source: PubMed
Publication date: 2026/01/14
Murakami YukiLam KamhungFukui ShinsukeHelmke ElizabethIczkowski Kenneth ALi YuejuSatake Noriko