DUSP7 antibody
- Known as:
- DUSP7 (anti-)
- Catalog number:
- orb101614
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Biorbyt biorb
- Gene target:
- DUSP7 antibody
Related genes to: DUSP7 antibody
- Gene:
- DUSP7 NIH gene
- Name:
- dual specificity phosphatase 7
- Previous symbol:
- -
- Synonyms:
- MKP-X, PYST2
- Chromosome:
- 3p21.2
- Locus Type:
- gene with protein product
- Date approved:
- 1997-03-19
- Date modifiied:
- 2016-10-05
Related products to: DUSP7 antibody
Related articles to: DUSP7 antibody
- Dual-specificity phosphatases (DUSPs) require two conserved motifs, the HCX₅R nucleophilic loop and a WPD/FPD-type general-acid loop, to support cysteine-dependent dephosphorylation. Although annotated as a DUSP, the catalytic competence of DUSP15 has remained ambiguous, with only weak activity reported against artificial substrates and paradoxical roles in sustaining ERK and Jak1-STAT3 signalling. Here, sequence analysis, crystallographic inspection, structural modelling, evolutionary profiling, interaction-network inference, and molecular dynamics (MD) simulations are integrated to reassess the functional properties of DUSP15. Motif analysis identifies two defining deviations: a phenylalanine immediately following the catalytic cysteine within a divergent HCFAGISR loop, and complete absence of a WPD/FPD-type general-acid loop. Structural examination of a DUSP15 crystal fragment, together with AlphaFold predictions, shows that the inserted phenylalanine projects into and sterically occludes the active-site cleft, in contrast to the open catalytic pocket of the active phosphatase DUSP7. Comparative analysis of 11 mammalian orthologs reveals absolute conservation of both anomalies, indicating long-standing selective maintenance of a catalytically divergent architecture. 100-ns all-atom MD simulations reveal a globally stable and compact fold with a conformationally rigid, tightly packed, and selectively dehydrated catalytic motif, lacking the flexibility and solvent accessibility typically required for productive cysteine-based catalysis. Comparative MD simulations performed under identical conditions further distinguish DUSP15 from the catalytically competent phosphatase DUSP7. Interaction-network analysis places DUSP15 within phosphatase-, transcriptional-, and metabolism-associated modules, consistent with scaffold-like regulatory roles. Together, these convergent structural, evolutionary, and dynamical features support a model in which DUSP15 functions predominantly as a non-catalytic adaptor, providing a mechanistic framework for its non-canonical regulation of ERK and Jak1-STAT3 signalling and its tumour-selective expression in chromophobe renal cell carcinoma. - Source: PubMed
Publication date: 2026/03/02
Sarhan Adil R - - Source: PubMed
Publication date: 2026/01/03
Tian HuiLi ShanshanGu XiupingLiang GaofengHe Jinxian - Solute carrier (SLC) is involved in diverse malignancies. This research analyzed the involvement of SLC-related genes in acute myeloid leukemia (AML). - Source: PubMed
Publication date: 2025/11/11
Zhang DeleiLi Gongli - Extranodal natural killer (NK)/T-cell lymphoma (ENKTL) is prevalent in the Asian population; however, little is known about its molecular characteristics. In this study, we examined the CD30 expression in ENKTLs and then performed whole exome sequencing on ten CD30 ENKTL and CD30 ENKTL paired samples. CD30 was positive in 55.74% of the ENKTLs. Single nucleotide and insertion/deletion polymorphism analyses revealed that 53.41% of the somatic mutations in CD30 ENKTLs were shared with CD30 ENKTLs, including mutations in SERPINA9, MEGF6, MUC6, and KDM5A. Frequently mutated genes were primarily associated with cell proliferation and migration, the tumor microenvironment, energy and metabolism, epigenetic modulators, vascular remodeling, and neurological function. PI3K-AKT, cAMP, cGMP-PKG, and AMPK pathways were enriched in both CD30 and CD30 ENKTLs. Copy number variation analysis identified a unique set of genes in CD30 ENKTLs, including T-cell receptor genes (TRBV6-1 and TRBV8), cell cycle-related genes (MYC and CCND3), immune-related genes (GPS2, IFNA14, TTC38, and CTSV), and a large number of ubiquitination-related genes (USP32, TRIM23, TRIM2, DUSP7, and UBE2QL1). BCL10 mutation was identified in 6/10 CD30 ENKTLs and 7/10 CD30 ENKTLs. Immunohistochemical analysis revealed that the expression pattern of BCL10 in normal lymphoid tissues was similar to that of BCL2; however, its expression in ENKTL cells was significantly higher (67.92% vs. 16.98%), implying the potential application of BCL10 inhibitors for treating ENKTLs. These results provide new insights into the genetic characteristics of CD30 and CD30 ENKTLs, and could facilitate the clinical development of novel therapies for ENKTL. - Source: PubMed
Publication date: 2024/08/24
Zhang XiaoyingLiang KeChen HaiyanLiu LongLiu RuiruiWang ChunxueZhang Cuijuan - Colorectal cancer (CRC) is one of the most prevalent and life-threatening cancers. Rapid cell proliferation is the leading cause of cancer-related death in CRC. MicroRNAs (miRNAs) have been identified to play essential roles in the proliferation of CRC. Differential expression of let-7c-5p in CRC was assessed using a GEO dataset, and confirmed through RT-qPCR using CRC subject tissues. Let-7c-5p-overexpressing HCT8 cell line was constructed by transfecting let-7c-5p. Bioinformatics analysis identified that DUSP7 is the target gene of let-7c-5p. Further experimental assays, including Cell Counting Kit-8 (CCK8), EdU staining, cell colony, and Western Blot assays, confirmed the target genes and pathway of let-7c-5p. Receiver operator characteristic curve (ROC) analysis was performed to evaluate the diagnostic value of let-7c-5p for CRC. Finally, survival analysis was performed to determine the effect of DUSP7 and let-7c-5p on the prognosis of CRC patients. RT-qPCR analysis showed that the expression level of let-7c-5p was significantly increased in CRC subject tissues compared to the adjacent tissue. Overexpression of let-7c-5p promoted cell proliferation in HCT8 cell line. Furthermore, the MAPK-ERK pathway's protein expression of p-ERK1/2 was downregulated, while the ratio of Bcl-2/Bax was increased by let-7c-5p transfection in HCT 8. ROC analysis demonstrated that the expressive level of let-7c-5p had higher diagnostic value for CRC. Survival curve analysis indicated that high expression of DUSP7 and low expression of let-7c-5p were associated with poor prognosis in CRC patients. The findings suggest that let-7c-5p exerts an antitumor function by inhibiting the DUSP7-mediated MAPK-ERK pathway. Both DUSP7 and let-7c-5p have the potential to serve as prognostic biomarkers in CRC patients. - Source: PubMed
Publication date: 2023/12/14
Fu JuanjuanZhou LongmeiLi SijinHe WenjingZheng JiningHou ZhipingHe Peiyuan