Glutamine PRPP amidotransferase antibody
- Known as:
- Glutamine PRPP amidotransferase (anti-)
- Catalog number:
- orb101760
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Biorbyt biorb
- Gene target:
- Glutamine PRPP amidotransferase antibody
Related genes to: Glutamine PRPP amidotransferase antibody
- Gene:
- PRB2 NIH gene
- Name:
- proline rich protein BstNI subfamily 2
- Previous symbol:
- -
- Synonyms:
- PRPPRB1, Ps, cP7
- Chromosome:
- 12p13.2
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2016-06-06
Related products to: Glutamine PRPP amidotransferase antibody
Related articles to: Glutamine PRPP amidotransferase antibody
- Peripheral artery disease (PAD) confers elevated risk for major adverse cardiovascular events (MACE), yet accurate risk stratification remains a challenge, particularly among patients with advanced disease necessitating endovascular revascularization. This study aimed to improve the prediction of MACE in a clearly defined high-risk PAD population (hospitalized patients undergoing endovascular intervention) by identifying novel protein biomarkers and developing a robust risk model. We prospectively analyzed blood samples from 164 hospitalized PAD patients scheduled for endovascular revascularization, employing untargeted plasma proteomics and metabolomics. Differential protein and metabolite profiles were compared between patients with and without subsequent MACE. Several proteins, including MMP3, MMP19, and PRB2, were markedly elevated in patients who developed MACE. A proteomics-based risk model incorporating these biomarkers achieved high discriminative accuracy (area under the curve > 0.80) for identifying individuals at increased risk. Metabolomic profiling revealed additional pathway alterations, notably involving tryptophan and glycogen metabolism, which provided mechanistic insights into cardiovascular complications but were not directly incorporated into the prediction model. This study demonstrates that integrating protein biomarkers markedly improves risk stratification in advanced PAD patients undergoing surgical intervention. The findings offer promising tools for early detection and enable more personalized management for this high-risk subgroup, while also deepening understanding of disease pathophysiology. However, further validation in larger and more diverse prospective cohorts is warranted before these findings can be broadly applied in clinical practice. - Source: PubMed
Publication date: 2026/05/06
Zhao WenxinMeng LingbingYan ShengLi PengSun YoujingGuo YamingZhang BowenCao Yifan Ren JunhongLi YongjunChen Zuoguan - The impact of diet during pregnancy on birth outcomes and child health is well established, and epigenetic changes may be one mechanism underlying such associations, but the role of meal timing (chrono-nutrition) is unclear. We conducted an epigenome-wide association study (EWAS) of maternal meal timing and placental DNAm (plaDNAm). Data came from 389 pregnant women in the Barcelona Life Study Cohort (BiSC). Chrono-nutrition and dietary data were collected at 20 weeks of pregnancy, and plaDNAm at delivery was characterized using the Illumina EPIC array. Linear robust regression models tested associations between five chrono-nutritional behaviors (time of first and last meal, nighttime fasting duration, number of eating occasions, and eating jetlag) and plaDNAm. We identified 7 CpGs significantly associated with time of last meal (Bonferroni p < 1E-08) and 63 suggestive CpGs (p < 1E-05). Hits included cg13147785 (E2F8), linked to placental cell cycle regulation, cg17665505 (DAP) and cg18303215 (ABCG5), associated with smoking and lung diseases in adults. To conclude, maternal chrono-nutrition was associated with some CpGs in the placenta, particularly time of last meal. Further studies are needed to clarify how meal timing may influence fetal development and long-term health through epigenetic mechanisms. - Source: PubMed
Llauradó-Pont JoanaAguilar-Lacasaña SofiaCosin-Tomas MartaJulvez JordiContreras-Rodríguez OrenKogevinas ManolisPapantoniou KyriakiGallo ElisaHarding Barbara NVrijheid MartineDadvand PayamBustamante MarionaLassale Camille - Serological screening, including immunological lateral flow assays, remains common for body fluid identification in sexual assault investigations but lacks the sensitivity and specificity of modern DNA profiling. To address this gap, alternative molecular approaches, including MS-based proteomics, have been explored. However, adoption is hindered by lengthy bottom-up workflows and reliance on research-grade instrumentation. Here, a streamlined, protease-free assay for the identification of saliva and seminal fluid in sexual assault evidence is described. Casework-type body fluid samples were extracted in a single step and analyzed by targeted DDA on a Q Exactive MS with a 25-min separation and data search using Byos software. The 96-well plate format used is amenable to higher-throughput automation. Discovery data sets included 50 saliva and 60 semen samples (including samples from 5 vasectomized males). This resulted in the identification of 7 saliva biomarkers (PRB1, PRB2, PRB4, PRH1, STATH, HTN1, and SMR3B) and 5 seminal fluid biomarkers (SEMG1, SEMG2, PSA, PAP, and PIP). Peptide standards were synthesized to confirm the discovery results and to develop a targeted assay. The method was successfully validated using 168 forensic casework-type samples, including diluted, laundered, and environmentally challenged samples on a variety of substrates. - Source: PubMed
Publication date: 2025/11/12
Brown Catherine OParker Glendon JWestring Christian GDanielson Phillip BLegg Kevin M - This research examined the effects of different probiotic dosages on three-month-old Guiqian semi-fine wool ram lambs (initial weight: 18.57 ± 0.96 kg). The lambs were randomly assigned to four dietary treatments: a control group (CON) received a standard basal diet, while three experimental groups, PRB1, PRB2, and PRB3, were supplemented with 1,000 mg/kg DMI, 2,000 mg/kg DMI, and 3,000 mg/kg DMI, respectively, of a probiotics blend (containing Bacillus subtilis and Bacillus licheniformis, each ≥ 1 × 10 CFU/g) over a 60-day period. The findings revealed that probiotics supplementation markedly improved final body weight and average daily gain (ADG), with the PRB2 group showing the most pronounced increase (P < 0.01). Compared with control lambs, probiotic-fed lambs presented significantly elevated serum Ig G and Ig A levels and reduced levels of the proinflammatory markers IL-6 and TNF-α (P < 0.05). The PRB2 group also presented increased Ig M, IL-10, and IL-1β concentrations (P < 0.05). Antioxidant profiling revealed that PRB1 lambs presented increased serum GSH-Px, T-AOC, and CAT activity, whereas PRB2 lambs presented further improvements in these markers plus elevated T-SOD and reduced MDA content (P < 0.05). Probiotics also modulated rumen ecology, lowering the NH-N concentration (P < 0.05) while increasing the total volatile fatty acid (TVFA) content. The PRB2 group notably altered the microbial composition, decreasing Bacteroidota and Verrucomicrobiota while increasing Firmicutes at the phylum level (P < 0.05). At the genus level, Christensenellaceae_R-7_group and Lachnospiraceae_NK3A20_group increased, whereas Prevotella, Succiniclasticum, Selenomonas, and Eubacterium_coprostanoligenes_group decreased (P < 0.05). The experimental results revealed that the addition of different doses of probiotics to the basal diet improved the growth performance, antioxidant capacity, and immunity of Guiqian semi-fine wool sheep and had a positive effect on the rumen microorganisms of Guiqian semi-fine wool sheep, and the best effect was achieved at the addition level of 2000 mg/kg. - Source: PubMed
Publication date: 2025/10/04
Peng YandiShen Xiaoyun - The performance of a pilot-scale HO-assisted permeable reactive barrier (PRB) system was evaluated for landfill leachate-contaminated groundwater remediation. HO pre-oxidation achieved 40-60 % COD removal at source concentrations > 75 mg/L. Carbon layers in PRB provided ∼42 % additional COD removal through catalyzed oxidation and adsorption, with BC+FeO (PRB2) outperforming AC (PRB1). BC+FeO also achieved 78.6 % NH removal vs. 30.8 % for AC), potentially through enhanced biological activity, thereby preserving zeolite ion-exchange capacity. After 8-month operation, 292.96 and 378.36 m of groundwater were remediated by PRB1 and PRB2, respectively. Effluent concentrations were reduced to10∼30 mg/L COD and < 1.5 mg/L NH, from source levels of 50-240 mg/L COD and 40-230 mg/L NH. Advection-diffusionadsorption model effectively described NH transport but underestimated early COD peaks due to complex oxidation. Coexisting contaminants reduced retardation factors by an order of magnitude. Temporary O accumulation and potential precipitation of CaCO and Fe/Mn hydroxides contributed to clogging in the front carbon layers, while biological activity and possible N gas accumulation dominated in zeolite and rear carbon layers. Hydraulic conductivity reductions reached 80 % in carbon and 50-75 % in zeolite layers but were operationally controllable. These findings provide critical design recommendations for implementing HO-assisted PRB systems in long-term landfill risk management. - Source: PubMed
Publication date: 2025/09/25
You YuqingZhan LiangtongSong XueZhao LiZhou Chen