SMAD6 monoclonal antibody (M08), clone 2E4
- Known as:
- SMAD6 mab (anti-) (M08), clonality 2E4
- Catalog number:
- H00004091-M08
- Product Quantity:
- 100 ug
- Category:
- -
- Supplier:
- Abno
- Gene target:
- SMAD6 monoclonal antibody (M08) clone 2E4
Related genes to: SMAD6 monoclonal antibody (M08), clone 2E4
- Gene:
- ANAPC15 NIH gene
- Name:
- anaphase promoting complex subunit 15
- Previous symbol:
- C11orf51
- Synonyms:
- HSPC020, DKFZP564M082, APC15
- Chromosome:
- 11q13.4
- Locus Type:
- gene with protein product
- Date approved:
- 2006-02-06
- Date modifiied:
- 2015-09-11
- Gene:
- KIAA0100 NIH gene
- Name:
- KIAA0100
- Previous symbol:
- -
- Synonyms:
- DKFZp686M0843, MGC111488, BCOX1, CT101, BCOX, FMP27
- Chromosome:
- 17q11.2
- Locus Type:
- gene with protein product
- Date approved:
- 2005-11-22
- Date modifiied:
- 2019-04-12
- Gene:
- SMAD6 NIH gene
- Name:
- SMAD family member 6
- Previous symbol:
- MADH7, MADH6
- Synonyms:
- HsT17432
- Chromosome:
- 15q22.31
- Locus Type:
- gene with protein product
- Date approved:
- 1997-08-22
- Date modifiied:
- 2015-08-26
- Gene:
- TMEM184B NIH gene
- Name:
- transmembrane protein 184B
- Previous symbol:
- C22orf5
- Synonyms:
- HS5O6A, DKFZP586A1024, FM08
- Chromosome:
- 22q13.1
- Locus Type:
- gene with protein product
- Date approved:
- 2000-03-29
- Date modifiied:
- 2016-10-05
Related products to: SMAD6 monoclonal antibody (M08), clone 2E4
Related articles to: SMAD6 monoclonal antibody (M08), clone 2E4
- Hair follicles, as key accessory organs in mammalian skin, are precisely regulated for growth and development influenced by genetic factors, hormones, and the local microenvironment. This study investigated the effect of dihydrotestosterone (DHT) on the proliferation of dermal papilla cells (DPCs) from Xinjiang Hetian sheep and its regulatory relationship with the TGF-β/Smad signalling pathway. DPCs were isolated from the back skin tissue of newborn Hetian sheep by collagenase II digestion, and their characteristics were identified by primary culture, passage, and double immunofluorescence staining with α-SMA and CD133 labelling. CCK-8 and EdU assays were used to evaluate the effect of varying DHT concentrations (0, 1, 10, 100, and 1000 nM) on DPC proliferation. The results showed that with the increased DHT concentration, cell proliferation also increased, and the highest rate was observed at 1000 nM. Furthermore, qPCR analysis revealed that 1000 nM DHT significantly upregulated Smad6 gene expression (p < 0.05), while it inhibited the expression of TGF-β1, TGF-βRII, and Smad5 (p < 0.05). These findings suggest that high DHT concentrations may promote DPC proliferation by inhibiting the TGF-β/Smad signalling pathway. This suggests a critical role of this pathway in regulating hair follicle development and hormone-dependent hair growth, providing evidence for the molecular mechanism of androgen action on dermal papilla cell function. - Source: PubMed
Wang QinqinLiu XuanFeng ZhiyaZhang JianpingLi ChunyangShi RuijunLi Shuwei - Bone morphogenetic proteins (BMPs), members of the transforming growth factor (TGF)-β superfamily, play essential roles in ovarian follicular development and steroidogenesis. Although inhibitory Smads (Smad6 and Smad7) negatively regulate BMP/TGF-β signaling, their roles in ovarian steroidogenesis remain unclear. Here, we investigated the effects of Smad6 and Smad7 on BMP-15-mediated steroidogenic regulation using human granulosa-like KGN cells and primary rat granulosa cells. Of note, siRNA-mediated knockdown of Smad6 enhanced BMP-15-induced Smad1/5/9 phosphorylation and Id-1 expression, whereas Smad7 knockdown increased basal Id-1 expression independently of BMP-15 in KGN cells. BMP-15 reduced progesterogenic StAR expression and this effect was enhanced by Smad6 or Smad7 knockdown. In addition, BMP-15 increased metabolization of progesterone by 20αHSD induction under Smad7-knockdown conditions, whereas aromatase expression was unaffected by BMP-15 regardless of Smad6 or Smad7 knockdown. In primary rat granulosa cells, combined knockdown of Smad6 and Smad7 significantly reduced basal and FSH-induced progesterone production without affecting estradiol production. Collectively, these results suggest that inhibitory Smad6 and Smad7 differentially and complementarily regulate BMP-15 signaling and progesterone synthesis in granulosa cells, contributing to the fine-tuning of ovarian BMP signaling and luteinization. - Source: PubMed
Publication date: 2026/09/15
Soejima YoshiakiYamamoto KoichiroIwata NahokoMotohashi KanonSuyama AtsuhitoNakano YasuhiroOtsuka Fumio - Primary ovarian insufficiency (POI) is a fertility disorder with a well-established genetic component, but many cases still remain idiopathic. Approximately 1.5-12% of patients with POI can carry a variant in the BMP15 gene, depending on the population and the diagnostic criteria. We hypothesize that genetic variations within pathways downstream of BMP15 activity in ovarian granulosa cells (GCs) may contribute to unexplained cases of POI. The main goal of this study is to identify novel variants associated with POI in genes induced by BMP15 in GCs. - Source: PubMed
Publication date: 2026/08/26
Rossetti RaffaellaFornili MarcoMoleri SilviaFerrari IlariaGentilini DavideCarbone ErikaPaffoni AlessioSomigliana EdgardoBiganzoli EliaMarozzi AnnaBrancati FrancescoMoretti CostanzoGiuliani CristinaPersani Luca - SMAD6 encodes an inhibitory SMAD protein that modulates BMP and TGF-β signaling. Heterozygous pathogenic variants in SMAD6 have been primarily associated with aortic valve disease, radioulnar synostosis, and nonsyndromic sagittal and metopic synostosis. However, only two syndromic patients with biallelic variants have been reported in the literature. We report a 4-year-old girl with neurodevelopmental delays, dysmorphic features, complex congenital heart disease, renal asymmetry, and arterial tortuosity. Whole exome sequencing showed two homozygous SMAD6 variants of uncertain significance: c.161G>T (p.Gly54Val) and c.1A>G (p.Met1?). This is the third patient with biallelic SMAD6 variants associated with skeletal changes, more complex cardiovascular phenotype, facial dysmorphism, and novel arterial abnormalities. This suggests biallelic variants may cause a distinct and potentially more severe autosomal recessive syndrome. Functional investigation is needed to determine the molecular consequences of biallelic SMAD6 variants and to inform variant classification and mechanism. This report characterizes a potential unique genetic syndrome associated with biallelic SMAD6 variants, highlighting the importance of additional sequencing, vascular imaging, and multidisciplinary care coordination for these patients. - Source: PubMed
Publication date: 2026/08/26
Nemshick MadisonOxman EliasLeon Eyby - The relatively low edible muscle yield and inconsistent flesh texture of red swamp crayfish limit its commercial value and highlight the need for effective nutritional interventions. Although L-carnosine has shown beneficial effects on muscle growth and quality in vertebrates, its nutritional functions and underlying mechanisms in crustaceans remain unclear. This study aimed to evaluate the effects of dietary L-carnosine on growth performance and muscle quality in red swamp crayfish (). A total of 324 red swamp crayfish, with an initial body weight of 6.19 ± 0.03 g, were randomly divided into 6 groups of three replicates and 54 crayfish per group (18 crayfish per replicate). Six experimental diets containing graded levels of L-carnosine (3.35, 59.28, 106.84, 222.20, 452.93, and 821.16 mg/kg) were fed for 8 weeks. Regression analysis revealed dose-dependent responses in multiple parameters. Weight gain rate, specific growth rate, protein efficiency ratio, protein deposition rate, muscle percentage, whole-body and muscle crude protein content, muscle textural properties (hardness and chewiness), hydroxyproline levels (alkaline-insoluble and total), myofiber density, small myofiber proportion (<50 μm), and total amino acid content exhibited quadratic trends ( < 0.05), with peak values observed in the 452.93 mg/kg group. Conversely, feed conversion ratio and large myofiber proportion (>70 μm) showed significant quadratic decreases ( < 0.05), reaching their lowest values at 452.93 mg/kg. Transcriptional analysis demonstrated that, compared with the 3.35 mg/kg L-carnosine group, the 452.93 mg/kg L-carnosine group significantly regulated genes involved in several key pathways: mTORC1 signaling (, , , , , and ), eIF2B-eIF2 signaling (), ubiquitin-proteasome system (, , , and ), autophagy-lysosomal system (, , , and ), TGFβ/Smads pathway (, , , , and ), and myogenic regulators (, , and ) ( < 0.05). These molecular modulations were consistent with observed phenotypic improvements. Overall, the findings demonstrated that a dietary L-carnosine level of 458.06 to 500.00 mg/kg, as determined by quadratic regression analysis, significantly enhances growth performance, feed utilization, muscle hardness and chewiness, and nutritive value in red swamp crayfish. - Source: PubMed
Publication date: 2026/08/05
Li XinyuanLiu YangyangXie ShouqiZhang JianminGao WeihuaJiang MingDong LixuePeng DiCheng KeHuang FengTian Juan