c_Myc
- Known as:
- c_Myc
- Catalog number:
- 1X-433-C100
- Product Quantity:
- 0.1 mg
- Category:
- -
- Supplier:
- Exbio
- Gene target:
- c_Myc
Related products to: c_Myc
9E10 c-mycActin regulatory protein CAP-G,Actin-capping protein GCAP39,Capg,Macrophage-capping protein,Mbh1,Mouse,Mus musculus,Myc basic motif homolog 1Ad-hOKSiM Human Oct4, Klf4, Sox2, and c-MycAd-hOKSiM/Human Oct4, Klf4, Sox2, and c-MycAd-mMKOS c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2Ad-mMKOS-GFP c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2, GFP tagAd-mMKOS-GFP/c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2, GFP tagAd-mMKOS/c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2Agarose Immobilized Goat anti-c-mycAgarose Immobilized Goat Anti-c-mycAgarose Immobilized Goat anti-c-myc PolyclonalAgie-bp1,Angiotensinogen gene-inducible enhancer-binding protein 1,DNA-binding protein AGIE-BP1,Hivep2,Human immunodeficiency virus type I enhancer-binding protein 2 homolog,Mibp1,MIBP-1,Myc intron-bialpha c-Myc antibodyalpha c-Myc antibodyalpha c-Myc antibody Polyclonal Antibodies Primary antibodies Related articles to: c_Myc
- Cisplatin (DDP) is a cornerstone chemotherapeutic agent for gastric cancer, yet its clinical application is often limited by severe adverse effects, particularly dose-dependent nephrotoxicity. Quercetin (Que) is a dietary flavonoid widely present in everyday foods such as onions, apples, tea, and broccoli. It has been reported to possess both renoprotective and antitumor properties. This study evaluated the therapeutic potential of combining DDP with Que in gastric cancer treatment. In vitro, CCK-8 assays, flow cytometry, and scratch wound tests were employed to assess the impact of Que on DDP-induced nephrotoxicity and chemosensitivity, while potential mechanisms were investigated through western blot analysis. In vivo, the combined regimen was tested in tumor-bearing nude mice by monitoring tumor growth and renal function. Our results showed that Que alleviated DDP-induced suppression of proliferation and apoptosis in HK-2 cells, indicating protection against nephrotoxicity. In comparison, in AGS gastric cancer cells, Que potentiated DDP-induced inhibition of proliferation and migration, and improved apoptosis. Mechanistically, Que inhibited ERK1/2 activation in both cell types. In HK-2 cells, ERK1/2 inhibition increased c-Myc expression and decreased cleaved caspase-3 levels, whereas in AGS cells, ERK1/2 inhibition reduced c-Myc and MMP-2 levels while elevating cleaved caspase-3 expression. These effects were reversed upon TBHQ treatment. In vivo, Que enhanced the antitumor efficacy of DDP while attenuating its nephrotoxicity. Collectively, these results indicate that Que achieves the abovementioned dual effect via cell type-specific modulation of the ERK1/2 pathway, supporting its potential as an adjuvant for gastric cancer chemotherapy. - Source: PubMed
Xia GenGu ShuningLi XiaojieLi KunLi Shizheng - The Kaposi's sarcoma-associated herpesvirus oncoprotein vIRF3 is essential for primary effusion lymphoma (PEL) cell survival and cooperates with cellular interferon regulatory factor 4 (IRF4) to activate super-enhancers (SEs) controlling MYC and IRF4. However, the DNA sequence requirements for this cooperation are unknown. Using reporter assays, we mapped vIRF3/IRF4 responsiveness within the IRF4-SE to an ∼83-bp element activated by vIRF3 alone and cooperatively by vIRF3 and IRF4. vIRF3-dependent activation required an AP-1 motif, whereas cooperation with IRF4 required IRF4 DNA binding and IRF-related motifs outside canonical AP-1-IRF composite elements (AICEs). These motifs were necessary but insufficient outside their native sequence context, indicating a requirement for extended flanking sequences. DNA pulldowns revealed distinct motif requirements for vIRF3 and IRF4 association with the IRF4-SE. A distal MYC-SE required a similarly extended region containing a functional AICE2 motif, with vIRF3 activity depending primarily on its AP-1 half-site. Motif requirements were preserved in integrated reporter assays. BATF promoted vIRF3/IRF4-dependent activation preferentially in integrated reporters and was required to maintain vIRF3 and IRF4 occupancy at the endogenous MYC-SE. These findings define context-dependent DNA and cofactor requirements through which vIRF3 co-opts IRF4-regulated oncogenic enhancers in PEL. - Source: PubMed
Liang ZiyanMagdongon Christine BMa Haocong KatherineHaynes Maureen ELu JingyiBartom Elizabeth TGottwein Eva - Uveal melanoma (UM) is driven by activating GNAQ/GNA11 mutations, predominantly at the hotspot Q209 residue. However, the functional impact of distinct GNAQ Q209 substitutions on downstream signaling and malignant progression remains unclear. Using immune-competent, multistep UM mouse models combining eye-restricted mutant GNAQ with BAP1 loss and MYC activation, we directly compared GNAQ and GNAQ in an otherwise matched genetic context. Tumors arising from either allele shared similar histology and cell-state composition. However, GNAQ tumors progressed faster and were associated with reduced survival. In contrast, GNAQ tumor-derived cell lines exhibited greater ex vivo fitness (proliferation, clonogenicity, and migration), whereas GNAQ-derived lines were relatively fragile in 2D culture. At the molecular level, GNAQ tumors preferentially engaged the FAK-YAP axis in primary lesions and derived cell lines. Together, these results support an allele-dependent, context-specific trade-off in which higher output GNAQ favors rapid in vivo expansion, while GNAQ promotes adaptability, potentially via FAK-YAP signaling, nominating allele-stratified therapeutic opportunities. - Source: PubMed
Jarajapu VineshNeelature Sriramareddy SathyaMecozzi NicolWang KaizhenKarreth Florian AXu Xiaonan - Cold stress represents a significant environmental challenge that constrains the development of the grapevine industry. The basic helix-loop-helix (bHLH) transcription factor family plays pivotal regulatory roles in plant growth, development, and stress responses. This study identifies a novel member of the bHLH family, referred to as VabHLH015, isolated from Vitis amurensis 'Zuoshan-1'. The physicochemical properties, conserved domains, and evolutionary relationships of VabHLH015 were thoroughly analyzed. Additionally, Arabidopsis thaliana and Vitis vinifera 'Chardonnay' calli overexpressing VabHLH015 were generated to evaluate their cold stress tolerance. Amino acid sequence analysis indicated that the VabHLH015 protein comprises 491 amino acids, containing the bHLH-MYC_N and bHLH_AtAIB_like domains. Tissue-specific expression analysis demonstrated differential expression of VabHLH015 across various grapevine organs, with transcript levels markedly increasing in response to low temperature and multiple exogenous hormones. Subcellular localization confirmed that VabHLH015 is a nuclear protein. A transcriptional activation assay showed that it possesses autoactivation activity. Functional analyses revealed that transgenic A. thaliana and grape calli overexpressing VabHLH015 exhibited heightened sensitivity to cold stress. In comparison to wild-type controls, these transgenic lines displayed significantly elevated malondialdehyde (MDA) content and electrolyte leakage, alongside diminished activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as reduced proline (Pro) accumulation. Moreover, the expression levels of several cold-responsive genes were significantly downregulated in the transgenic lines. In summary, the overexpression of VabHLH015 attenuates plant tolerance to low temperatures by modulating the expression of cold tolerance-related genes and antioxidant enzyme activities. This study offers a novel candidate gene and provides a theoretical framework for elucidating the molecular mechanisms underlying cold tolerance, facilitating the breeding of cold-tolerant grapevine varieties. - Source: PubMed
Publication date: 2026/10/04
Guo NingningLi ShanLi ChengnanZhao YuleiMa WenlingYin Xiao - Environmental metal exposure has been increasingly recognized as a contributor to cardiovascular toxicity, yet the effects of single and mixed metal burdens, as well as susceptible population subgroups, remain insufficiently characterized. This study aimed to investigate the associations of blood copper (Cu), zinc (Zn), Cu/Zn ratio, selenium (Se), lead (Pb), cadmium (Cd), total mercury (HgT), and manganese (Mn) with cardiovascular disease (CVD), and to evaluate the joint association of multiple metal exposures and potential heterogeneity across population subgroups. Data from 1,967 participants (211 CVD cases) in the 2011-2016 NHANES were analyzed. Spearman correlation, logistic regression, restricted cubic spline (RCS), weighted quantile sum (WQS) regression, and quantile-based g-computation (QGcomp) were applied to assess associations of single metals and mixtures with CVD. Subgroup analyses were conducted by demographics and health conditions. Network pharmacology was applied to identify candidate pathways and toxicological targets. CVD patients had higher levels of Cu, Cu/Zn ratio, Pb, and Cd, but lower levels of Zn, HgT, and Mn. Spearman analyses showed Cd (rho = 0.114) and Pb (rho = 0.111) have positive associations with CVD, whereas Mn has a negative connection (rho=-0.069) (all P < 0.05). In quartile-based logistic regression, Cd showed the most consistent positive association with CVD, with participants in the highest quartile having higher odds of CVD in the fully adjusted model (OR = 2.29, 95% CI: 1.37-3.87, P = 0.002). The Cu/Zn ratio was positively associated with CVD in partially adjusted models, but the association was attenuated and no longer statistically significant after full adjustment (OR = 1.55, 95% CI: 0.94-2.57, P = 0.089). RCS revealed linear correlations between Pb, Cd and Mn with prevalent CVD. The WQS index was positively associated with higher odds of CVD (OR = 1.70, 95% CI: 1.16-2.52, P = 0.008), with Cd, the Cu/Zn ratio, and Pb showing the largest positive weights. QGcomp similarly indicated a positive overall mixture association (OR = 1.64, 95% CI: 1.10-2.45, P = 0.014), while Mn contributed in the negative direction. Subgroup analyses indicated that specific metals exerted positive contributions in vulnerable populations, including younger males, smokers, drinkers, elderly, hypertensive, and low-BMI groups. GAPDH, AKT1, CTNNB1, MYC, TNF, and TP53 were predicted to be key targets. Elevated blood Cd showed the most consistent positive association with CVD across individual and mixture-based analyses. The Cu/Zn ratio and Pb also contributed substantially to the positive association of the metal mixture with CVD, although the independent association of the Cu/Zn ratio was attenuated after full multivariable adjustment. Mn showed a potential inverse association. These findings highlight the toxicological relevance of metal mixtures in cardiovascular disease and underscore the need for mechanistic studies to establish causality. - Source: PubMed
Publication date: 2026/10/03
Jin XianduWu HaoZhang HanmoJin YanyeFeng YurongLiu YueLi JiaoWei LipingQi ZhiQi Xin