CD63
- Known as:
- CD63
- Catalog number:
- 11-343-C025
- Product Quantity:
- 0.025 mg
- Category:
- -
- Supplier:
- Exbio
- Gene target:
- CD63
Related genes to: CD63
- Gene:
- CD63 NIH gene
- Name:
- CD63 molecule
- Previous symbol:
- MLA1
- Synonyms:
- ME491, TSPAN30
- Chromosome:
- 12q13.2
- Locus Type:
- gene with protein product
- Date approved:
- 1988-08-07
- Date modifiied:
- 2016-01-15
Related products to: CD63
Related articles to: CD63
- Cancer cells confronting oxidative stress must coordinate their extracellular vesicle (EV) secretion to balance intercellular signaling with the intracellular programs required for survival, yet how these decisions are integrated remains poorly understood. Here, we identify a stress-adaptive mechanism in which stress granules (SGs) selectively suppress CD63 EV release. Using a bioluminescent EV-reporter screen, we found that the clinical compound YM155 selectively inhibits CD63 EV secretion across diverse tumor cells. Mechanistically, YM155 rapidly inactivates the antioxidant transcription factor FOXO3a, diminishing expression of key detoxifying enzymes and leading to delayed but sustained accumulation of reactive oxygen species (ROS). Elevated ROS drives SG formation, and these SGs function not as passive storage sites but as RNA triage hubs that exclude and destabilize a subset of transcripts. Among them, Rab27A mRNA-encoding a GTPase essential for multivesicular-body docking to the plasma membrane-is selectively excluded and degraded, resulting in loss of Rab27A protein and suppression of CD63 EV secretion. Forced Rab27A expression restores EV release but paradoxically reduces proliferation under oxidative stress, indicating that EV suppression is prosurvival. The same FOXO3a-ROS-SG-Rab27A axis operates during physiological glucose deprivation and is evident in vivo, where SGs form in xenograft tumors and circulating CD63 EVs decline. Pancancer transcriptomic analyses further show that Rab27A expression correlates with FOXO3a-dependent antioxidant programs, underscoring clinical relevance. These findings reveal that SGs actively reprogram RNA fate to tune vesicle output, establishing a redox-responsive mechanism by which cancer cells transiently suppress EV secretion to enhance survival. - Source: PubMed
Publication date: 2026/07/06
Dong YueYoshida TakeshiMaeda MitsuyoAdachi HarukiEguchi AsamiMishiro-Sato EmiOkuzaki DaisukeKataoka YoskyYoshida TakuyaOneyama Chitose - Extracellular vesicles (EVs) are emerging as valuable biomarkers in clinical diagnostics. Yet, the natural biological variation in EV concentration and phenotype among healthy individuals and across longitudinal time points remains insufficiently characterized, complicating interpretation and reproducibility in biomarker studies. - Source: PubMed
Publication date: 2026/06/19
Kløve-Mogensen KirstineBæk RikkeRasmussen Rikke WSøndergaard Evo K LHandberg AaseMellergaard MaikenJørgensen Malene M - Tear extracellular vesicles (tEVs) are a promising source of biomarkers for ocular and systemic diseases. Standardized approaches for their collection and isolation remain underdeveloped. Contact lenses (CLs), routinely used for vision correction and as bandage lenses, offer a practical platform for clinically integrated tear sampling. Using healthy rabbit eyes ( = 4), we compared two tear collection methods, daily soft CLs and Schirmer Strips (SS), and two EV isolation techniques, ExoQuick (EQ) polymer precipitation and ultracentrifugation (UC). tEVs were characterized by particle counts, protein content, zeta potential, mRNA expression of reference genes (GAPDH, SNRPG, TOMM7), and exosomal tetraspanins (CD9, CD63). CL-derived tEVs showed lower protein contamination and reduced inflammatory cytokine expression, whereas SS-derived tEVs contained more cellular debris and higher levels of MMP-9, TNF-α, and Leptin, consistent with tissue stress and remodeling. EQ yielded higher particle recovery and smaller aggregates than UC, but produced vesicles with more positive surface charge, indicating altered surface properties and reduced colloidal stability. GAPDH demonstrated the most stable expression across samples, and CD63 exhibited higher eye-specific RNA scores. These findings provide a methodological framework for tEV profiling, highlighting complementary molecular information captured by CLs and SS and trade-offs between EQ and UC for tEV recovery and stability. - Source: PubMed
Publication date: 2026/07/03
Boychev NikolaySushma Mudigunda VYeung VincentRoss Amy ECiolino Joseph B - Skeletal muscle development is strongly influenced by crosstalk between adipose tissue and muscle, yet the underlying molecular mechanisms in Ovis aries remain insufficiently defined. This study investigated the regulatory effects of adipocyte-derived exosomes on sheep primary myoblasts. Co-culture with adipocytes significantly enhanced myoblast proliferation, as indicated by increased cyclin-dependent kinase 4 (CDK4), proliferating cell nuclear antigen (PCNA), and Cyclin D1 expression, while simultaneously suppressing differentiation via reduced myogenin (MYOG), myogenic differentiation 1 (MYOD), and myosin heavy chain (MYHC) levels. Exosomes isolated from mature adipocytes (30-150 nm), expressing TSG101, CD63, and CD9, were effectively internalized by myoblasts and reproduced these effects. RNA sequencing identified circ_0000002 as one of the most abundant circular RNAs (circRNAs) in adipocyte-derived exosomes. Functional assays demonstrated that circ_0000002 promoted myoblast proliferation and inhibited differentiation. Mechanistically, circ_0000002 acted as a competing endogenous RNA (ceRNA) by sponging miR-27a, thereby relieving miR-27a-mediated repression of myostatin (MSTN). Dual-luciferase reporter assays confirmed direct interactions between circ_0000002 and miR-27a and between miR-27a and the MSTN 3' untranslated region (3´UTR). Co-transfection experiments further validated that the ceRNA-like mechanism of circ_0000002/miR-27a/MSTN regulates myoblast differentiation. In a cardiotoxin (CTX)-induced tibialis anterior injury mouse model, intramuscular administration of adipocyte-derived exosomes impaired muscle regeneration and increased MSTN expression, supporting the in vivo relevance of this pathway. Collectively, our findings reveal that exosomal circ_0000002 regulates sheep myoblast differentiation via miR-27a/MSTN ceRNA pathway. This work provides the first evidence that an adipocyte-derived exosomal circRNA mediates fat-muscle communication and highlights a potential target for improving muscle growth in sheep. - Source: PubMed
Liang LinZhang WeipengYu ShilongChen HaoranYan JianchenHuo JianrongZhao Junxing - Can non-coding RNAs in exosomes from IVF embryo culture media serve as non-invasive markers for evaluating embryo quality? - Source: PubMed
Publication date: 2026/01/16
Zhang LingyingMahemuti MairepatiYakupu ZulihumaerLi ChengyuMaimaiti YusupujiangPang MinGan XiaojingZhao QiongzhenHuang WeidongJiapaer Zeyidan