Ask about this productRelated genes to: CBP80 antibody
- Gene:
- NCBP1 NIH gene
- Name:
- nuclear cap binding protein subunit 1
- Previous symbol:
- NCBP
- Synonyms:
- CBP80, Sto1
- Chromosome:
- 9q22.33
- Locus Type:
- gene with protein product
- Date approved:
- 1995-01-05
- Date modifiied:
- 2016-10-05
Related products to: CBP80 antibody
Related articles to: CBP80 antibody
- The rise in global bacterial resistance necessitates the discovery of novel antibiotics. Plant-derived Antimicrobial Peptides (AMPs) offer structural diversity and biocompatibility. This study aims to investigate the green synthesis and biological activities of derivatives of NCBP, a linear non-classical AMP identified from plants. - Source: PubMed
Geng ZiyingZhang LinyanLi HaidiLiang Taigang - Subcellular stress profoundly influences protein synthesis. However, both the nature of spatiotemporally restricted chemical cues and local protein responders to these cues remain elusive. Unlocking these mechanisms requires the ability to functionally map in living systems locale-specific stress responder proteins and interrogate how chemical modification of each responder impacts proteome synthesis. We resolved this problem by integrating precision localized electrophile generation and genetic code expansion tools. Upon examination of four distinct subcellular locales, only nuclear-targeted electrophile stress stalled translation. We discovered that NCBP1-a nuclear-resident protein with multifaceted roles in eukaryotic mRNA biogenesis-propagated this nuclear stress signal through a single cysteine (C436) from among its 19 conserved cysteines. This NCBP1(C436)-specific modification elicited alternative splicing of more than 250 genes. Mechanistically, global protein synthesis stall was choreographed by impaired association between electrophile-modified NCBP1(C436) and SF3A1, an essential component of spliceosome, triggering the production of alternatively spliced S6 kinase, whose expression was sufficient to dominantly inhibit protein translation. - Source: PubMed
Publication date: 2026/02/10
Chang DaluAssari MahdiSuwathep ChananyaSappakhaw KhomkritUttamapinant ChayasithLong Marcus J CAye Yimon - Cassava production in sub-Saharan Africa is severely impacted by diseases. Most pathogens require interaction with host susceptibility factors to complete their life cycles and cause disease. Targeted DNA methylation is an epigenetic strategy to alter gene expression in plants, and we previously reported that a zinc-finger fused to DMS3 could establish methylation at the promoter of , a bacterial susceptibility gene, and this resulted in decreased disease. Here, we attempt a similar strategy for cassava brown streak disease. This disease is caused by the ipomoviruses CBSV and UCBSV. These viruses belong to the family , which has been shown extensively to require host eIF4E-family proteins to infect plants and cause disease. We previously found that cassava plants with simultaneous knockout mutations in two genes, , resulted in decreased susceptibility to CBSD. Here, we report successful simultaneous targeting of both promoters with methylation using a dCas9-DRMcd-SunTag system. However, in contrast to our previous work with controls indicate that CRISPR interference is occurring in these lines and is sufficient for the reduction of gene expression. Future research will use genetic crosses to segregate away the DNA methylation reagents and, if DNA methylation proves heritable, assess whether methylation alone is sufficient to increase resistance to CBSD. - Source: PubMed
Publication date: 2026/02/06
Lin Zuh-Jyh DanielHernandez Gabriela LStanton Myia KZheng XingguoGilbert Kerrigan BVeley Kira MJensen GregYoder MarisaFeng SuhuaGhoshal BasudevGardiner JasonWang MingJacobsen Steven ECarrington James CBart Rebecca S - Preimplantation embryogenesis requires precise synchronization of transcriptional activation, mRNA export and translation, and metabolic reprogramming to sustain developmental requirements. Nuclear cap-binding protein 1 (NCBP1), a conserved subunit of the cap-binding complex, has established roles in mRNA processing and export in somatic cells, but its potential functions in preimplantation embryogenesis remain undefined. The spatiotemporal expression dynamics of Ncbp1 were explored on multiple levels. After microinjecting interfering RNA at zygotic stage to knockdown Ncbp1, embryonic developmental competence was evaluated. Co-injection of small interfering RNA and in vitro transcribed Ncbp1 mRNA into the zygote was used to rescue the knockdown phenotype. Further, poly-adenylated RNA-fluorescence in situ hybridization, RNA sequencing, and quantitative proteomics were used to investigate the effects of Ncbp1 knockdown. In addition, oleic acid (OA) supplementation was used to rescue developmental abnormalities. NCBP1 exhibited dynamic spatiotemporal expression coinciding with nuclear-to-cytoplasmic translocation of protein from morula stage. Depletion of Ncbp1 caused morula arrest or fragmentation, accompanied by nuclear poly-adenylated RNA retention and down-regulation of lipid metabolic pathways, notably, stearoyl-CoA desaturase 1 (SCD1), a key enzyme generating monounsaturated OA. Exogenous OA supplementation partially rescued blastocyst formation, implicating NCBP1 in the regulation of SCD1-OA-mediated metabolic homeostasis during morula-to-blastocyst transition. This study illustrates NCBP1 as a mediator that regulates RNA export and lipid homeostasis during early mouse embryo development. Especially NCBP1 regulates the SCD1-OA metabolic pathways, ensuring metabolic flexibility essential for successful morula-to-blastocyst transition, thereby providing new insights into the molecular basis of embryonic developmental competence. - Source: PubMed
Liu YujunHe YimingWang XiangfeiWang NanWang XiaomengWang YuqianChen LuZhu XiaohuiQiao JieYuan PengYan Liying - The N7-methylguanosine (m7G) modification is known as a common post-transcriptional modification of RNA that has been found to be involved in the pathogenesis of various diseases. However, its role in osteoarthritis (OA) remains largely unknown. This study aimed to identify the genes associated with m7G modification in OA and further investigate their diagnostic value and immune infiltrates. - Source: PubMed
Publication date: 2025/10/07
Sun HongChen KunhaoXiong ZhilinZhuang YongLiu MiaoNing XuYang Hua