Ask about this productRelated genes to: YBX1 antibody
- Gene:
- YBX1 NIH gene
- Name:
- Y-box binding protein 1
- Previous symbol:
- NSEP1
- Synonyms:
- YB-1, YB1, DBPB, NSEP-1, MDR-NF1, BP-8, CSDB, CSDA2
- Chromosome:
- 1p34.2
- Locus Type:
- gene with protein product
- Date approved:
- 1993-10-20
- Date modifiied:
- 2016-10-05
Related products to: YBX1 antibody
Related articles to: YBX1 antibody
- Y-box binding protein 1 (YB-1; encoded by ) is a multifunctional DNA- and RNA-binding protein implicated in cell cycle regulation, DNA repair, stress adaptation, and therapy resistance. Elevated expression has been associated with aggressive disease across multiple cancer types; however, its pan-cancer genomic and clinical correlates, and the extent to which these reflect proliferative activity versus genomic instability, remain incompletely defined. Here, we performed an integrative pan-cancer analysis across 53 independent datasets spanning 33 tumour types, incorporating transcriptomic ( mRNA), proteomic (RPPA), genomic, and clinical data. We found that is rarely altered at the genomic level, whereas its mRNA expression is highly variable within tumour cohorts. Tumours with high mRNA expression consistently exhibited conserved transcriptional programmes enriched for cell cycle, mitotic, RNA processing, and signalling pathways, patterns that were also reflected at the protein level by concordant pathway associations with elevated YB-1 abundance. These molecular features co-occurred with clinicopathological characteristics indicative of aggressive disease. High mRNA expression was associated with increased mutation burden, chromosomal alteration burden, hypoxia, and homologous recombination deficiency at the pan-cancer level, with similar molecular associations observed in tumours stratified by elevated YB-1 protein levels. The association between expression and chromosomal alteration burden was largely attenuated after accounting for proliferative activity, particularly G2/M-associated transcriptional programmes used as a proxy for mitotic activity. While the relationship with mutation burden was heterogeneous across tumour types, this pattern suggests that links between expression and chromosomal instability primarily reflect shared proliferative and mitotic tumour biology rather than an independent genomic instability programme. Clinically, high mRNA expression was associated with advanced disease stage, higher histologic grade, reduced progression-free survival, and poorer overall survival. Elevated YB-1 protein levels were also associated with advanced disease stage and poorer survival outcomes and demonstrated a similar, although non-significant, directional trend with histologic grade. Collectively, these findings demonstrate that elevated expression marks proliferative and clinically aggressive tumour states within which genomic instability-related features arise in a context-dependent manner, providing a clarified pan-cancer framework for interpreting YB-1-associated tumour biology. - Source: PubMed
Publication date: 2026/05/13
Wang SelenaShafaei Pishabad ZahraSarkar DebinaBhandarkar Apeksha ArunSarwar MakhdoomJeffs AaronReid GlenBraithwaite AntonyMehta Sunali - The cerebral cortex drives human cognition through the coordinated activity of discrete cortical areas, each harboring specialized molecular, structural and functional characteristics. Central to this organization is the prefrontal cortex (PFC), a hub for executive function that displays disproportionate expansion in humans and selective vulnerability to neurodevelopmental disorders. Previous work has identified a collection of PFC-enriched marker genes with dynamic expression trajectories, and re-analysis of these datasets converge these markers into 18 distinct molecular signatures of spatiotemporal PFC identity. However, the intrinsic gene networks that coordinate these molecular signatures to shape the human PFC remains unclear. Through pooled CRISPR activation screens in human primary cortical tissues, we have evaluated the ability of PFC-enriched transcription factors to intrinsically pattern PFC molecular identity. Our screens identify novel roles for the neurogenesis regulator, YBX1, in the activation of human PFC fate. In parallel screens and knock-down experiments in human cortical organoids, we define how YBX1 acts in concert with other PFC determinants to activate molecular signatures of PFC identity. Our findings support a model in which PFC patterning is orchestrated by cohorts of intrinsic determinants that initiate, potentiate, and modulate PFC gene signatures, conferring robustness to the development of the human PFC. - Source: PubMed
Publication date: 2026/05/14
Nano Patricia RJaklic Daniel CGiang VannaSoto Jose AMil JessenyaWang SeanMartija AntoniWick BrittneyHaeussler MaximilianBhaduri Aparna - MicroRNAs are dysregulated in patients with idiopathic nonobstructive azoospermia (iNOA), but the underlying mechanisms remain unclear. This study investigated miRNA-processing regulators in iNOA pathogenesis using bioinformatics and experimental validation in 41 obstructive azoospermia (OA) and 51 iNOA patients. Primary human spermatogonial stem cells (SSCs) were isolated for functional studies. YBX1, a miRNA-binding protein, was identified to be reduced in testes and SSCs of patients with iNOA. Knockdown of YBX1 in SSCs inhibited the processing of pre-miR-17 and the transcription of primary miR-221/222 (pri-miR-221/222), reducing mature miR-17 and miR-221/222 levels. This led to upregulated KIT and PTEN, inhibited PI3K/AKT signaling, and induced apoptosis. Additionally, YBX1 depletion reduced oxygen consumption and glycolysis, increased reactive oxygen species (ROS) production, and elevated the Bax/Bcl2 ratio, which was partially reversed by miR-17 and miR-221/222 supplementation. YBX1 loss disrupts miRNA biogenesis, leading to spermatogenic failure via apoptotic and metabolic dysfunction. - Source: PubMed
Publication date: 2026/04/17
Zhu YingMao GenhongGeng XujingDou QianXiang YungaiMa LiyingWei LeiTan Li - Despite the expression of multiple transcript isoforms from a gene, conventional gene expression analyses assume that a single transcript is expressed from each gene. We analyzed the transcript isoforms expressed in gonadotropin-induced mouse mural and cumulus granulosa cells (mGCs and cGCs) isolated from antral follicles to elucidate the potential mechanism of differentiation. Considering that either a single transcript or multiple isoforms are expressed from genes, we identified differential expression of about 70% of transcripts between mGCs and cGCs. Although the differential expressions were similar, the single-transcript-wise differentially expressed genes did not correlate with their corresponding differentially expressed transcript isoforms. We identified transcript isoforms of key transcriptional regulators in ovaries, including Chd1, Ezh2, Kdm5a/5b, Gata4, Esr2, Fos, Myc, and Ybx1, that were not identified in single-transcript-based analyses. Further analysis revealed a transcript switch in more than 30% of the differentially expressed isoforms. While one or more transcript isoforms of Cebpa, Dnmt3a, Pgr, Rest, Runx1, and Sirt1 were switched off, those of Brd7, Chd1, Med21, Nfkbia, Rbm39, Suv39h2, Tcf12, Xist, and Ybx3 were switched on in cGCs. Interestingly, several genes, including Dab2, Ezh2, Gata4, Gnas, Gtf2i, Klf10, Setdb1, and Sp3, exhibited at least one isoform that was switched off and another that was switched on in cGCs. Transcript switching was primarily due to alternative splicing, followed by alternative transcription start sites and polyadenylation sites. We also identified differential expression of the potential regulators of such transcript switching in cGCs. Our results suggest that transcript switching may play an important role in mural and cumulus granulosa differentiation, a key insight that would remain unknown without mRNA isoform analysis. - Source: PubMed
Publication date: 2026/05/23
Shila SharminPei Grace JBahadursingh ElizabethPeramsetty NikiDahiya VineshMarsh Courtney AThiyagarajan RamkumarZhang MeijiaFields Patrick ERumi M A Karim - Testicular germ cell tumors (TGCTs) are the most common solid malignancies in young men, yet effective biomarkers and therapeutic targets remain limited. The role of lncRNAs in TGCT remains poorly understood at single-cell resolution. - Source: PubMed
Publication date: 2026/05/22
Cao JianZhu FangXu KongrongLiu ZhizhongChen LeiLv ShanshanFan LiqingGuo JieTao MinaLuo YanweiLi GuoliBo Hao