Ask about this productRelated genes to: UIMC1 antibody
- Gene:
- UIMC1 NIH gene
- Name:
- ubiquitin interaction motif containing 1
- Previous symbol:
- -
- Synonyms:
- RAP80
- Chromosome:
- 5q35.2
- Locus Type:
- gene with protein product
- Date approved:
- 2006-10-27
- Date modifiied:
- 2014-11-19
Related products to: UIMC1 antibody
Related articles to: UIMC1 antibody
- Circadian rhythms regulate physiological and behavioural processes, with the light-dark cycle acting as the principal environmental cue synchronising the biological clock; disruption of this cue can affect brain function and behaviour. Using zebrafish (Danio rerio) as a translational model, we examined how chronic photoperiod alteration, applied from the larval stage to adulthood, affects exploratory activity, anxiety-like behaviour, aggression, and social preference, and whether these changes are paralleled by shifts in gut microbiota composition and brain transcriptomic profile. Zebrafish were reared under three photoperiod regimes: 14L/10D (control), 20L/4D (extended light), and 4L/20D (extended dark), followed by behavioural, gut metagenomic, and brain transcriptomic analyses. Extended darkness (4L/20D) reduced anxiety-like behaviour, whereas extended light (20L/4D) increased aggression both extended photoperiods altered social behaviour, but with distinct behavioural profiles. Gut microbiota in the 20L/4D group showed phylum-level co-dominance of Actinomycetota and Pseudomonadota, including a greater presence of potentially pathogenic taxa, while the 4L/20D group was dominated by Bacillota. Brain transcriptome profiling, based on a single pooled sample per condition, identified transcripts showing the largest expression differences in each group: in 20L/4D, transcripts linked to phototransduction (gnat1, saga, pde6ga, exorh), ribosomal function (rpl13a, rpl9, knop1), melatonin synthesis (asmt), calcium signalling (plcb4a), and glycolysis (eno1a) were elevated; in 4L/20D, transcripts linked to neuroprotection (nr4a1), DNA repair and chromatin remodelling (fance, uimc1, histh1l), synaptic plasticity (serpina10a), neurodevelopment (six6a), and cell-cycle regulation (btg2) were elevated. These findings indicate that photoperiod shapes zebrafish behaviour, gut microbial composition, and brain gene expression, providing a hypothesis-generating basis for future studies incorporating biological replication. - Source: PubMed
Publication date: 2026/09/30
Sivarajan DhanushaPothayi VidyaDevasia Sebastian ChempakasseryRamachandran Binu - Deubiquitylases modulate cellular processes by removing monoubiquitin or cleaving polyubiquitin chains. The ARISC-RAP80 complex partners with BRCA1-BARD1 to form the BRCA1-A supercomplex, which recognises K63-linked ubiquitin chains at DNA damage sites. ARISC-RAP80 contains multiple ubiquitin-binding sites, yet how these influence recognition and cleavage of K63-polyubiquitylated substrates remains unknown. We discover that a composite three-subunit interface allows ARISC-RAP80 to position K63-linked polyubiquitin chains in its catalytic site. Substrate recognition is further supported by RAP80 and non-catalytic ubiquitin-binding sites that impose a compact conformation on K63-polyubiquitylated substrates. This mechanism exploits the inherent flexibility of long ubiquitin chains and differs considerably from other deubiquitylases. Structure-guided mutageneses validate ubiquitin chain interactions, and cell-based assays demonstrate a functional role of the observed interfaces in chromatin recruitment. Our findings define mechanisms of polyubiquitin chain decoding and cleavage by ARISC-RAP80, linking ubiquitin reading and erasing functions to BRCA1-A mediated DNA damage responses. - Source: PubMed
Publication date: 2026/08/11
Foglizzo MartinaDatta ArindamDegtjarik OksanaPerera HirunikaLiburd JordanSykora Upasana MGanji Sri RanjaniWildsmith GemmaChandler FrancescaCampbell Lisa JCalabrese Antonio NGreenberg Roger AZeqiraj Elton - In response to DNA double-strand breaks (DSBs), 5'-3' resection is required for production of single-stranded DNA (ssDNA) and commitent of homologous recombination (HR). Here, we demonstrated that CDW19S, a DSB-bound 19S proteasome variant, coordinates phasic control of long-range resection in a spatial-separated manner. Phase I exploits a panel of ubiquitin modifications on RAP80 (6Kub) as a hesitation mechanism to restrain BRCA1 loading. The deubiquitinase POH1, an integral component of CDW19S, removes 6Kub to allow BRCA1 assembly with BRCA1-A complex and firing of extensive resection. Following the action of phase I apparatus that is metazoan-specific, evolutionarily conserved phase II takes the relay in more distal compartments by imposing CDW19S-engaged CRL4WDR70 E3 ligase to degrade ADRM1, leading to the full-range ssDNA production dedicated for HR activation. The phasic regulation stimulates the repositioning of 53BP1-dependent resection barriers: 6Kub removal and BRCA1 loading overcome the 53BP1/PTIP barrier in phase I, and the demolition of ADRM1 antagonizes 53BP1/RIF1. Importantly, the phasic control of extensive resection serves for the tight control of ssDNA production, securing HR activation and preventing toxic repair mechanisms. Aggregately, our work reveals a coordinative function of CDW19S facilitating sufficient end resection that is crucial for error-free DNA repair. - Source: PubMed
Zeng MingTang ZizhiLi ChunyiRen LaifengWang XiaojunCarr AntonyLiu Cong - Receptor-associated protein 80 (RAP80) is a key component of the BRCA1-A complex and plays an essential role in recruiting this complex to DNA double strand break (DSB) sites to facilitate homologous recombination (HR) repair. The function of RAP80 in DNA damage repair is tightly regulated by post-translational modifications (PTMs). However, the detailed mechanisms by which PTMs modulate RAP80 activity remain to be fully elucidated. Here, we demonstrate that SENP3 dynamically regulates DSB repair by targeting RAP80. Mechanistically, upon DNA damage, RAP80 undergoes SUMOylation and is recruited to DSB sites, promoting subsequent BRCA1 recruitment. SENP3 may facilitate the timely dissociation of RAP80 from DNA damage sites during the later stage, thereby promoting proper repair progression and ensuring timely termination of the DNA damage response. Dysregulation of RAP80 SUMOylation due to depletion impairs HR repair and increases cellular sensitivity to irradiation and chemotherapy drugs. Collectively, our findings identify SENP3 as a key regulator of DNA repair through its dynamic control of RAP80-BRCA1 complex formation and DNA damage repair processes. - Source: PubMed
Fu JianfengWei MinXu RuidanZheng Xiaofeng - Growing evidence has identified potential biomarkers of cognitive-behavioural therapy (CBT) efficacy in obsessive-compulsive disorder (OCD). Genetic and epigenetic mechanisms (e.g., polymorphisms, DNA methylation) contribute to OCD pathogenesis and CBT response variability, establishing them as a key research focus. To evaluate their associations with CBT outcomes in OCD, we conducted a systematic review of PubMed, Web of Science, CNKI, and Cochrane Library (from inception to January 2025), identifying eight studies that met rigorous inclusion criteria. The identified predictors included: (1) Genetic polymorphisms (BDNF); (2) Epigenetic modifications (DNA methylation of MAOA, SLC6A4, OXTR, PIWIL1, MIR886, PLEKHA1, KCNQ1, TRPM8, HEBP1, HTR7P1, MAPK8IP3, ENAH, RABGGTB (SNORD45C), MYEF2, GALK2, CEP192, and UIMC1). These markers may influence neural plasticity, neurotransmitter regulation, and related processes, providing molecular substrates for the observed treatment effects. Converging evidence suggests that distinct neurocognitive mechanisms may mediate CBT efficacy in OCD, particularly fear extinction learning and goal-directed behaviors (GDBs), which we analyze mechanistically. Future studies should integrate polygenic risk scores (PRS) with functional neuroimaging to dissect individual variability in CBT response, mainly through cortico-striato-thalamo-cortical (CSTC) circuit profiling. To our knowledge, this is the first systematic review synthesizing genetic and epigenetic predictors of CBT response in OCD; these findings provide compelling evidence for biomarkers for CBT personalization in OCD, advancing a novel precision psychiatry framework. - Source: PubMed
Publication date: 2025/05/03
Chen YuWang PengchongLi Zhanjiang