Ask about this productRelated genes to: THAP2 antibody
- Gene:
- THAP2 NIH gene
- Name:
- THAP domain containing 2
- Previous symbol:
- -
- Synonyms:
- DKFZP564I0422
- Chromosome:
- 12q21.1
- Locus Type:
- gene with protein product
- Date approved:
- 2003-07-21
- Date modifiied:
- 2016-11-10
Related products to: THAP2 antibody
Related articles to: THAP2 antibody
- To systematically analyze the expression profiles of long non-coding RNAs (lncRNAs) in serum-derived exosomes from patients with age-related hearing loss (ARHL), and to further identify key regulatory lncRNAs involved in the pathogenesis and progression of ARHL. Peripheral blood samples were collected from patients with ARHL and age-matched normal-hearing controls. Serum was separated and exosomes were extracted. The exosomes were identified by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and Western blot. Subsequently, total RNA was extracted from the purified exosomes for lncRNA transcriptome sequencing. Based on the sequencing results, we identified differentially expressed lncRNAs and mRNAs and conducted multi-dimensional functional analysis, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome pathway database (Reactome), and Disease Ontology (DO). Finally, four key mRNAs (, , , and ) and four differentially expressed lncRNAs (DE-lncRNAs), namely MSTRG.150961.7, ENSG00000273015, MSTRG.336598.1, and ENSG00000273493, were experimentally verified by quantitative real-time polymerase chain reaction (RT-qPCR) technology. Exosomes were successfully isolated from serum and confirmed by particle size, morphological examination, and the expression of exosome-labeled proteins. A total of 2874 DE-lncRNAs were identified, among which 988 were downregulated and 1886 were upregulated. Similarly, 2132 DE-mRNAs were detected, among which 882 were downregulated and 1250 were upregulated. GO analysis revealed significant enrichment in biological processes such as "phospholipid binding", "phosphatidylinositol binding", "phosphatase binding", "phosphatidylinositol bisphosphate binding", "phosphatidylinositol-4,5-bisphosphate binding", "phosphatidylinositol-3,5-bisphosphate phosphatase activity". KEGG is significantly enriched in signaling pathways including "Wnt signaling pathway", "Hippo signaling pathway", "Cushing syndrome", and "Nucleocytoplasmic transport". The functional annotations of Reactome were significantly enriched in biomolecular pathways including "tRNA processing", "Cellular response to heat stress", "Extra-nuclear estrogen signaling", "Metabolism of non-coding RNA", and "CTNNB1 T41 mutants aren't phosphorylated". DO is significantly enriched in diseases or pathological conditions such as "hepatitis", "bacterial infectious disease", "cystic fibrosis", and "vasculitis". , , , and may serve as potential candidate biomarker for ARHL. Additionally, lncRNA MSTRG.150961.7, lncRNA MSTRG.336598.1, and lncRNA ENSG00000273493 may play significant roles in the pathogenesis of this condition. - Source: PubMed
Publication date: 2026/01/12
Yang GuijunXie ZhongqinHuang YuKe JingTang ZiyiChen ZhijiKuang ShaojingLi FeixianLuo HuanLai QinWang BoZhang JuhongYuan Wei - The conifer 'Zhongshanshan' ( 'Zhongshanshan') is characterized by rapid growth, strong stress resistance, and high ornamental value and has significant potential for use in afforestation, landscaping, and wood production. The main method of propagating 'Zhongshanshan' is tender branch cutting, but the cutting rooting abilities of different 'Zhongshanshan' clones differ significantly. To explore the causes of rooting ability differences at a molecular level, we analyzed the transcriptome data of cutting base and root tissues of 'Zhongshanshan 149' with a rooting rate of less than 5% and 'Zhongshanshan 118' with rooting rate greater than 60%, at the developmental time points in this study. The results indicated that differentially expressed genes between the two clones were mainly associated with copper ion binding, peroxidase, and oxidoreductase activity, response to oxidative stress, phenylpropanoid and flavonoid biosynthesis, and plant hormone signal transduction, among others. The expression pattern of was different throughout the development of the adventitive roots of the two clone cuttings. Therefore, this gene was selected for further study. It was shown that was a nuclear-localized transcription factor and demonstrated a positive feedback effect on rooting in transgenic cuttings. Thus, the results of this study explain the molecular mechanism of cutting rooting and provide candidate gene resources for developing genetic breeding strategies for optimizing superior clones of 'Zhongshanshan'. - Source: PubMed
Publication date: 2024/02/19
Liu JiaqiXuan LeiYu ChaoguangHua JianfengWang ZiyangYin YunlongWang Zhiquan - Bone marrow mesenchymal stem cell-derived exosomes (BMSC-exos) may function as novel candidates for treating diabetic wounds due to their ability to promote angiogenesis. - Source: PubMed
Ruan HongruShi HuiLuan WenkangPan Sida - Scutellaria baicalensis is a commonly used Chinese medicinal herb. In this study, we identified the germplasm resources of commercial S. baicalensis samples based on trnH-psbA, petA-psbJ, and ycf4-cemA sequences according to the available chloroplast genome sequencing results, and measured the content of baicalin by HPLC. Through the above means we determined the best DNA barcode that can be used to detect the germplasm resources and evaluate the quality of commercial S. baicalensis samples. A total of 104 samples were collected from 24 provinces, from which DNA was extracted for PCR amplification. The amplification efficiencies of trnH-psbA, petA-psbJ, and ycf4-cemA sequences were 100%, 59.62%, and 25.96%, respectively. The results of sequence analysis showed that 5, 4, and 2 haplotypes were identified based on trnH-psbA, petA-psbJ, and ycf4-cemA sequences, respectively. However, the sequences of haplotypes in commercial samples were different from that of the wild type, and the joint analysis of three fragments of S. baicalensis only identified 6 haplotypes. Furthermore, the phylogenetic analysis and genetic distance analysis indicated that trnH-psbA could be used to identify S. baicalensis from adulterants. The above analysis showed that trnH-psbA was the best fragment for identifying the germplasm resources of commercial S. baicalensis samples. We then analyzed the haplotypes(THap1-THap5) of commercial S. baicalensis samples based on trnH-psbA and found that THap2 was the main circulating haplotype of the commercial samples, accounting for 86.55% of the total samples, which indicated the scarce germplasm resources of commercial S. baicalensis samples. The content of baicalin in all the collected commercial S. baicalensis samples exceeded the standard in Chinese Pharmacopoeia and had significant differences(maximum of 12.21%) among samples, suggesting that the quality of commercial S. baicalensis samples varied considerably. However, there was no significant difference in baicalin content between different provinces or between different haplotypes. This study facilitates the establishment of the standard identification system for S. baicalensis, and can guide the commercial circulation and reasonable medication of S. baicalensis. - Source: PubMed
Zhang Jing-HanLiu Shan-HuZhang Zhi-FeiShi YueMan Jin-HuiYin Guang-YaoWang XinLiu Feng-BoWang Xiao-HuiWei Sheng-Li - MicroRNAs (miRNA) are small non-coding RNAs that play an important role in the control of gene expression by inhibiting protein translation or promoting messenger RNA degradation. Today, miRNAs have been shown to be involved in various physiological and pathological cellular processes, including cancer, where they can act as oncogenes or tumor suppressor genes. Recently, lowered expression of miR-100, resulting in upregulation of FGFR3, has been correlated with low-grade, non-invasive bladder urothelial cancer, as an alternative oncogenesis pathway to the typical FGFR3 gene mutation. Our aim is to analyze the role of miR-100 in bladder cancer cell lines in controlling the expression of some of its possible target genes, including FGFR3 and its relationship with proliferation, apoptosis and DNA ploidy. - Source: PubMed
Publication date: 2014/11/26
Morais Denis RReis Sabrina TViana NayaraPiantino Camila BerfortMassoco CristinaMoura CaioDip NelsonSilva Iran ASrougi MiguelLeite Katia Rm